BLACK BANGLE (ZINGIBER OTTENSII VAL.) RHIZOME AND KATUK LEAVES (SAUROPUS ANDROGYNUS L. MERR) EXTRACT COMBINATION PROTECTIVE ROLE ON ADIPOSE TISSUES HISTOLOGIC PROFILE OF HIGH-FAT AND CARBOHYDRATE DIET-INDUCED OBESE MALE RATS

AGUS SULAEMAN; Patonah; GHINA GRECIA NEGARA

doi:10.22159/ajpcr.2018.v11s1.26613

Authors

AGUS SULAEMAN Department of Pharmacology, Bandung School of Pharmacy, Jl. Soekarno Hatta No.754, Cipadung Kidul, Panyileukan, Bandung, Indonesia.
Patonah Department of Pharmacology, Bandung School of Pharmacy, Jl. Soekarno Hatta No.754, Cipadung Kidul, Panyileukan, Bandung, Indonesia.
GHINA GRECIA NEGARA Department of Pharmacology, Bandung School of Pharmacy, Jl. Soekarno Hatta No.754, Cipadung Kidul, Panyileukan, Bandung, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11s1.26613

Keywords:

Zingiber ottensii Val, Sauropus androgynus (L) Merr, Histology score, Adipose tissue

Abstract

Â

Â Objective: The effect of Zingiber ottensii Val. rhizome and Sauropus androgynus L. Merr leaves extract combination was investigated using histologic profile of adipose tissues in obese male rats induced by high-fat and carbohydrate diets.

Methods: This was a preventive study, conducted for 42 days by simultaneous administration of diets and extracts administration. The subjects were divided into 8 groups. All groups except negative control group were fed with high-fat and carbohydrate diets. Orlistat, metformin, and curcumin were used as contrast.

Result: The phytochemical screening of Z. ottensii Val. rhizome extract showed the presence of flavonoids, saponins, and triterpenoids, meanwhile S. androgynus L. Merr leaves extract presented flavonoids, tannins, saponins, steroids, and triterpenoids. The results showed tissues histological differences in all test group compared with positive control. The most effective combination dose for bangle and katuk leaves extract in protecting adipose tissue was 100 mg/Kg:100 mg/Kg body weight.

Conclusion: The combination of black bangle and katuk leaves extract showed a protective role, demonstrated by adipose tissues histologic profile.

Downloads

Download data is not yet available.

References

Lawrence J, et al. The Interaction of free Fatty Acid, Total Antioxidant Status, Plasminogen Activator Inhibitor-1, Adiponectin and high sensitivity C-Reactive Protein on the Occurrence of Impaired Glucose Tolerance, Dissertation, Hasanuddin University Makassar; 2005.

Dewoto, Hedi R. Development of Indonesian traditional medicine to phytofarmaka. Indones Med Mag 2007;57: ???.

Wijayakusuma HM, Dalimarta S, Wirian AS. Medicinal Plant from Indonesia. Jakarta: Kartini Library Publisher; 1997.

Rukmana R, Indra MH. Katuk Leaves. The potencial and Benefit. Yogyakarta: Kanisius; 2003.

Ministry of Health Republic of Indonesia. Indonesian Medical Material. Vol. VI. Jakarta: Ministry of Health Republic of Indonesia; 1996.

Hasimun P, Adnyana IK, Valentina R, Lisnasari E. Potential alpha-glucosidase inhibitor from selected zingiberaceae family. Asian J Pharm Clin Res 2016;9:164-7.

Susanti NM, Budiman IN, Warditiani NK. Phytochemical screening ethanol extract of katuk leaves (Sauropus androgynus (L.) Merr.). Journal of Faculty of Mathematical and Life Science. Vol. 2. Surakarta: Udayana University; 2014.

Utami A, Meryalita R, Prihatin NA, Ambarsari L, Kurniatin PA, Nurcholis W, et al. DNA Methods Variation of Temulawak Leaves (Curcuma xhanthorriza. Roxb). Indonesian: Proceeding of UNESA National Seminar of Chemistry; 2012.

Kim C. Severity of ultrasonographic liver steatosis and metabolic syndrome in Korean men and women. World J Gastroentrol 2005;11:5314-21.