EFFECT OF DILLENIA INDICA L. AGAINST OXIDATIVE STRESS-INDUCED CARDIOMYOPATHY ON ALLOXAN-INDUCED DIABETES MICE MODEL

Authors

  • Plabita Sahariah Department of Biochemistry, North Eastern Hill University, Shillong – 793 022, Meghalaya, India.
  • Jutishna Bora Department of Biochemistry, North Eastern Hill University, Shillong – 793 022, Meghalaya, India.
  • Donkupar Syiem Department of Biochemistry, North Eastern Hill University, Shillong – 793 022, Meghalaya, India.
  • Surya Bhan Department of Biochemistry, North Eastern Hill University, Shillong – 793 022, Meghalaya, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i8.26636

Keywords:

Dillenia indica, Alloxan, Antihyperglycemic, Antioxidative

Abstract

 Objective: The aim of the present study is to investigate the antihyperglycemic and antioxidative properties of Dillenia indica fruits.

Methods: Aqueous fruit extract and methanolic fruit extract (MFE) were prepared, and preliminary phytochemical screening was carried out. Diabetic mice were prepared with alloxan (150 mg/kg) body weight (b.w.). Antihyperglycemic study of short duration was carried out with doses (150–550) mg/kg b.w. of MFE in diabetic mice. Antioxidant enzymes (superoxide dismutase, catalase, and glutathione reductase) activity assays and histopathological analysis were done in heart tissue of mice.

Results: Preliminary phytochemical screening showed that the phytoconstituents were strongly present in the MFE and therefore was considered for further studies. From the antihyperglycemic study, it was found that 350 mg/kg b.w. dose was the most effective in reduction of blood glucose level. A significant increase in the activities of the antioxidant enzymes was observed in the MFE-treated group. From the histopathological studies, it was observed that detrimental effects of oxidative stress were attenuated in the treated group.

Conclusion: Concluding the studies, it could be ascertained that D. indica fruits were found to be quite effective in proving its potential against hyperglycemia and oxidative stress, and therefore, the fruits could be considered to be of therapeutic value in diabetes.

 

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References

Mamun AN, Hossain MD, Hassan N, Dash BP, Sapon MA, Sen MK. A review on medicinal plants with antidiabetic activity. J Pharm Phytochem 2014;3:149-59.

Patar AK, Bhan S, Syiem DK. Effect of chlorophyllin, an semi-synthetic chlorophyll molecule on hyperglycemia and hyperlipidemia in streptozotocin induced diabetic mice. Int J Pharm Pharm Sci 2016;8:293-6.

Shettar AK, Vedamurthy AB. Studies on in vitro antidiabetic activities of Hopea ponga and Vitex leucoxylon. Int Pharm Pharm Sci 2017;9:263 7.

Diabetes Control and Complications Trial Research Group. The relationship of a glycemic exposure (HbA1c) to the risk of development and progression of retinopathy in the Diabetes Control and Complications Trial. Diabetes 1995;44:968-83.

Klein R. Hyperglycemia and microvascular disease in diabetes. Diabetes Care 1995;18:258-68.

Stratton IM, Adler AI, Neil HA, Metthews DR, Manley SE, Cull CA, et al. UK Prospective Diabetes Study Group. Association of glycaemia with macrovascular and microvascular complications of type 2 diabetes (UKPDS 35). BMJ 2000;321:405-12.

Zoungas S, Chalmers J, Ninomiya T, Li Q, Cooper ME, Colagiuri S, et al. Advance Collaborative Group. Association of HbA1c levels with vascular complications and death in patients with Type 2 diabetes: Evidence of glycemic thresholds. Diabetologia 2012;55:636-43.

Brownlee M. Biochemistry and molecular cell biology of diabetic complications. Nature 2001;414:813-20.

Seok MS. Reactive oxygen and nitrogen species in pathogenesis of vascular complications of diabetes. Diabetes Metab J 2012;36:190-8.

Prabu M, Kumuthakalavalli R. Antidiabetic potential of the oyster mushroom Pleurotus florida (Mont.) Singer. Int J Curr Pharm Res 2017;9:51-4.

Saravanamuttu S, Sudarsanam D. Antidiabetic plants and their active ingredients: A Review. Int J Pharm Sci Res 2012;3:3639-50.

Annapurna A, Mahalakshmi DK, Krishna KM. Antidiabetic activity of a polyherbal preparation (tincture of panchparna) in normal and diabetic rats. Indian J Exp Biol 2001;39:500-2.

Mamun AN, Hossain MD, Hassan N, Dash BP, Sapon MA, Sen MK. A review on medicinal plants with antidiabetic activity. J Pharm Phytochem 2014;3:149-59.

Patar AK, Bhan S, Syiem DK. Effect of chlorophyllin, an semi-synthetic chlorophyll molecule on hyperglycemia and hyperlipidemia in streptozotocin induced diabetic mice. Int J Pharm Pharm Sci 2016;8:293-6.

Shettar AK, Vedamurthy AB. Studies on in vitro antidiabetic activities of Hopea ponga and Vitex leucoxylon. Int Pharm Pharm Sci 2017;9:263 7.

Diabetes Control and Complications Trial Research Group. The relationship of a glycemic exposure (HbA1c) to the risk of development and progression of retinopathy in the Diabetes Control and Complications Trial. Diabetes 1995;44:968-83.

Klein R. Hyperglycemia and microvascular disease in diabetes. Diabetes Care 1995;18:258-68.

Stratton IM, Adler AI, Neil HA, Metthews DR, Manley SE, Cull CA, et al. UK Prospective Diabetes Study Group. Association of glycaemia with macrovascular and microvascular complications of type 2 diabetes (UKPDS 35). BMJ 2000;321:405-12.

Zoungas S, Chalmers J, Ninomiya T, Li Q, Cooper ME, Colagiuri S, et al. Advance Collaborative Group. Association of HbA1c levels with vascular complications and death in patients with Type 2 diabetes: Evidence of glycemic thresholds. Diabetologia 2012;55:636-43.

Brownlee M. Biochemistry and molecular cell biology of diabetic complications. Nature 2001;414:813-20.

Seok MS. Reactive oxygen and nitrogen species in pathogenesis of vascular complications of diabetes. Diabetes Metab J 2012;36:190-8.

Prabu M, Kumuthakalavalli R. Antidiabetic potential of the oyster mushroom Pleurotus florida (Mont.) Singer. Int J Curr Pharm Res 2017;9:51-4.

Saravanamuttu S, Sudarsanam D. Antidiabetic plants and their active ingredients: A Review. Int J Pharm Sci Res 2012;3:3639-50.

Annapurna A, Mahalakshmi DK, Krishna KM. Antidiabetic activity of a polyherbal preparation (tincture of panchparna) in normal and diabetic rats. Indian J Exp Biol 2001;39:500-2.

Bhattaram VA, Ceraefe M, Kohlest C, Vest M, Deundorf H. Pharmacokinetics and bioavailabitlity of herbal medicinal products. Phytomedicine 2002;9:1-33.

Kumar S, Kumar V, Prakash OM. Free radicals scavenging effect of Dillenia indica leaves. Asian J Pharm Biol Res 2011;1:169-73.

Yeshwante SB, Juvekar AR, Pimprikar RB, Kakade RT, Tabrej M, Kale MK, et al. Anti-diarrheal activity of methanolic and aqueous extracts of Dillenia indica leaves. Res J Pharmacol Pharm 2009;1:140 2.

Sharma HK, Chhangte L, Dolui AK. Traditional medicinal plants in Mizoram, India. Fitoterapia 2001;72:14-61.

Harborne J. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. 3rd ed. London: Chapman and Hall; 1998.

OECD. OECD Guidelines for Testing of Chemicals-425. Acute Oral Toxicity Up-and-Down Procedure. OECD; 2001.

Rumanti RM, Nainggolan M, Harahap U. Phytochemical screening and antidiabetic activity of different leaf extracts from lotus (Nelumbo nucifera gaertn.) in streptozotocin induced mice. Asian J Pharm Clin Res 2017;10:190-2.

Farnsworth NR. Biologycal and phytochemical screening of plants. J Pharm Sci 1996;55:225-76.

Harbone JB. Metode Fitokimia. Vol. 2. Bandung: ITB; 1987. p. 49.

Graham JM. Homogenization of mammalian tissues. Sci World J 2002;2:1626-9.

Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976;72:248-54.

Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for SOD. Eur J Biochem 1974;47:469-74.

Aebi H. CAT in vitro. In: Methods Enzymol. New York, USA:Academic Press; 1984.

Carlsberg I, Mannervik B. Glutathione reductase. Methods Enzymol 1985;113:484-90.

Kiernan JA. Histological and Histochemical Methods: Theory and Practice. 3rd ed. Oxford, England: Butterworth-Heinemann Publishers; 1999.

Bailey CJ. New pharmacological approaches to glycemic control. Diabetes Rev 1999;7:94-113.

Islam MA, Akhtar MA, Khan MR, Hossain MS, Alam MK, Wahed MI, et al. Antidiabetic and hypolipidemic effects of different fractions of Catharanthus roseus (Linn.) on normal and streptozotocin-induced diabetic rats. J Sci Res 2009;1:334-44.

Alphonse A, Mohan VR, Doss A. Antidiabetic activity of Bacolepis Nervosa (Wight and Arn.) Decne.exmoq. extract on alloxan induced diabetic rats. Int J Pharm Pharm Sci 2016;8:320-6.

Armagan A, Uz E, Yilmaz HR, Soyupek S, Oksay T, Ozcelik N. Effects of melatonin on lipid peroxidation and antioxidant enzymes in streptozotocin-induced diabetic rat testis. Asian J Androl 2006;8:595 600.

Hsu WT, Tsai LY, Lin SK, Hsiao JK, Chen BH. Effects of diabetes duration and glycemic control on free radicals in children with type 1 diabetes mellitus. Ann Clin Lab Sci 2006;36:174-8.

Kale MA, Bindu SM, Khadkikar P. Role of antioxidants and nutrition in oxidative stress: A review. Int J Appl Pharm 2015;7:1-4.

Indravati A, Asyarie S, Suciati T, Retnoningrum DS. Study on the properties of purified recombinant superoxide dismutase from Staphylococcus equorum, a local isolate from Indonesia. Int J Pharm Pharm Sci 2014;6:440-5.

Abdille MH, Singh RP, Jayaprakash GK, Jena BS. Antioxidant activity of the extracts from Dillenia indica fruits. Food Chem 2005;90:891-6.

Published

07-08-2018

How to Cite

Sahariah, P., J. Bora, D. Syiem, and S. Bhan. “EFFECT OF DILLENIA INDICA L. AGAINST OXIDATIVE STRESS-INDUCED CARDIOMYOPATHY ON ALLOXAN-INDUCED DIABETES MICE MODEL”. Asian Journal of Pharmaceutical and Clinical Research, vol. 11, no. 8, Aug. 2018, pp. 445-9, doi:10.22159/ajpcr.2018.v11i8.26636.

Issue

Vol 11 Issue 8 August 2018

Section

Original Article(s)

The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.

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