NEOPTERIN AND INTERFERON-GAMMA AS IMMUNE RESPONSE MARKERS IN BETATHALASSEMIA MAJOR PATIENTS

Shahad F Obeid; Sanad B Al-a'araji; Bassam F Matti; Hayder Adnan Fawzi

doi:10.22159/ajpcr.2018.v11i9.26661

Authors

Shahad F Obeid Department of Biochemistry, College Science for Women, University of Baghdad, Baghdad, Iraq.
Sanad B Al-a'araji Department of Biochemistry, College Science for Women, University of Baghdad, Baghdad, Iraq.
Bassam F Matti Haematology Specialist, Medical City Complex, Baghdad Teaching Hospital, Baghdad, Iraq.
Hayder Adnan Fawzi Clinical Pharmacist, Medical City Complex, Baghdad Teaching Hospital, Baghdad, Iraq. http://orcid.org/0000-0001-9970-0119

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i9.26661

Keywords:

Thalassemia major, Interferon-gamma, Neopterin

Abstract

Objective: The assessment of neopterin and interferon-gamma (IFN-Î³) levels as a part of immune system response about serum iron status in Î²-thalassemia (TM) major patients.

Methods: Spectrophotometry applied for the estimation of iron status including serum iron level, total iron-binding capacity, and unsaturated iron-binding capacity. Enzyme-linked immunosorbent assay (ELISA) applied for the estimation of the serum cytokines included neopterin and IFN-Î³ also serum ferritin estimation by ELISA from 130 Î²-TM major patients where they divided according to serum ferritin level (< and â‰¥2500 ng/mL).

Results: The neopterin and IFN-Î³ concentration showed significantly increased with direct correlation among TM patient group when compared to the normal healthy control group. However, there was no significant difference between different levels of serum ferritin.

Conclusion: The increased serum level of neopterin and IFN-Î³ found in patients with Î²-TM may be due to the direct effect of iron on cellular immune cells beside of immune system response to the effect of iron toxicity on different body organs. There was a direct moderate relationship between neopterin and IFN-Î³.

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Author Biographies

Shahad F Obeid, Department of Biochemistry, College Science for Women, University of Baghdad, Baghdad, Iraq.

MSC. Biochemistry

Sanad B Al-a'araji, Department of Biochemistry, College Science for Women, University of Baghdad, Baghdad, Iraq.

PhD, Biochemistry

Bassam F Matti, Haematology Specialist, Medical City Complex, Baghdad Teaching Hospital, Baghdad, Iraq.

MBChB, FICMS, Haematology specialist

Hayder Adnan Fawzi, Clinical Pharmacist, Medical City Complex, Baghdad Teaching Hospital, Baghdad, Iraq.

B.Sc, FICMS

Clinical pharmacist, Baghdad Medical City Hospital

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