PROTECTIVE ACTIVITY OF ASPARAGUS RACEMOSUS IN METHOTREXATE-INDUCED LIVER TOXICITY IN WISTAR RATS

Arul Daniel J; Susmita Das; Neethu Jayan; Asha Devi S

doi:10.22159/ajpcr.2018.v11i9.26728

Authors

Arul Daniel J Department of Biomedical Sciences, School of Bioscience and Technology, Vit, Vellore, Tamil Nadu, India.
Susmita Das Department of Biomedical Sciences, School of Bioscience and Technology, Vit, Vellore, Tamil Nadu, India.
Neethu Jayan Department of Biomedical Sciences, School of Bioscience and Technology, Vit, Vellore, Tamil Nadu, India.
Asha Devi S Department of Biomedical Sciences, School of Bioscience and Technology, Vit, Vellore, Tamil Nadu, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i9.26728

Keywords:

Hepatotoxicity, Lipid peroxidation, Methotrexate, Reactive oxygen species, Asparagus racemosus

Abstract

Objectives: Various clinically available drugs along with the beneficial action also have drastic side effects due to chronic exposure. In liver, these resulting side effects can be over production of reactive oxygen species, which will further lead to oxidative stress and hepatotoxicity. Therefore, as a preventive measure, the protective role of herbal extracts is being evaluated because of its high success rate and low toxic effects. The primary aim of this study was to evaluate the efficiency of the protective role of Asparagus racemosus is evaluated and studied against methotrexate (MTX)-induced hepatic damage in male Wistar albino rats.

Methods: The course of the study was for 14 days. During this experimental study, the animals were categorized into four groups with six rats per group. Group I (positive control) which was treated with normal saline, Group II (negative control) with MTX 20 mg/kg of body weight on 12th day, Group III with A. racemosus 300 mg/kg of body weight + MTX 20 mg/kg on 12th day, and Group IV with A. racemosus 100 mg/kg of body weight + MTX 20 mg/kg on 12th day. On 14th day, the animals were sacrificed, and histopathological as well as antioxidant assays were performed.

Results and Conclusion: Assays revealed high lipid peroxidation level and low antioxidant levels in Group II. Meanwhile, in Group III and IV, the levels were restored near to control, which supported the protective role of A. racemosus against MTX-induced hepatic damage. Histopathology evaluation also supported the above-mentioned findings.

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