PROPHYLACTIC EFFICACY OF BACOPA MONNIERI ON DECABROMODIPHENYL ETHER (PBDE-209)-INDUCED ALTERATIONS IN OXIDATIVE STATUS AND SPATIAL MEMORY IN MICE
Abstract
Bacopa monnieri (BM) is extensively used as a nerve tonic in traditional medicine systems, originated from India and neighbouring countries. BM possesses antioxidative and memory enhancing properties. The present study has been designed to investigate the neuroprotective role of Bacopa monnieri against PBDE-209-induced alterations in spatial memory and oxidative status in the frontal cortex and hippocampus of mice. The mice were orally administered with PBDE-209 at the dose of 20 mg/kg bw from PND 3-10. BM at the doses of 40, 80 or 120 mg/kg body weight (bw) were co-administered with 20 mg/kg of PBDE-209 from PND 3-10. The spatial memory of young mice were evaluated by Morris water maze (MWM) and 8-armed radial maze (RAM). We also tested cellular oxidants e.g. MDA (malondialdehyde), protein carbonyl (PC) and antioxidant enzymes e.g. superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) in the frontal cortex (FC) and hippocampus (Hc) of mice at neonate (PND 11) and young age (PND 60). Supplementation with BM significantly restored the levels of MDA and protein carbonyls in FC and Hc, which were elevated significantly after PBDE-209 exposure. PBDE-209-induced decrease in the activities of antioxidant enzymes recovered significantly following supplementation with BM. Further, BM-supplementation resulted in significant improvement in the working and reference memory of PBDE-209 exposed mice. The findings, therefore, suggest the neuroprotective efficacy of BM against PBDE-209-induced impairments in spatial memory and oxidative status.
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