IN VITRO ANTIFUNGAL ACTIVITY OF FLOWER EXTRACT OF PANDANUS ODORATISSIMUS AGAINST DERMATOPHYTIC FUNGI

Geethavani Babu; Balamuruganvelu Singaravelu; Sreenivasalu Reddy Vallapu; Srikumar Ramasundaram

doi:10.22159/ajpcr.2018.v11i9.27082

Authors

Geethavani Babu Department of Microbiology, Bharath University, Bharath Institute of Higher Eduction and Research, Chennai, Tamil Nadu, India.
Balamuruganvelu Singaravelu Department of Microbiology, DM Wayanad Institute of Medical Sciences, Wayanad and Bharath University, Bharath Institute of Higher Eduction and Research, Chennai, Tamil Nadu, India.
Sreenivasalu Reddy Vallapu Department of Microbiology, Bharath University, Bharath Institute of Higher Eduction and Research, Chennai, Tamil Nadu, India.
Srikumar Ramasundaram Department of Microbiology,Centre for Research, Sri Lakshmi Narayana Institute of Medical Sciences, Bharath University, Bharath Institute of Higher Eduction and Research, Chennai, Tamil Nadu, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i9.27082

Keywords:

Dermatophytes, Antifungals, Agar-well diffusion method, Anti-dermatophytic activity, Medicinal plant, Pandanus odoratissimus

Abstract

Objective: The present study aimed to evaluate the antifungal activity of Pandanus odoratissimus oil against dermatophytic fungi, and it was compared against the two commonly used antifungal agent's fluconazole and griseofulvin.

Methods: A total of seven strains of dermatophytes were tested for antifungal activity using oil extracted from the flower of P. odoratissimus by using agar-well diffusion method and the zone of inhibition was compared with antifungal agent's fluconazole and griseofulvin. Minimum inhibitory concentration (MIC) was determined using the tube-dilution method.

Results and Conclusion: The zone of inhibition varied from 16.32 to 19.76 mm for fluconazole, 12.12â€“18.16 mm for griseofulvin, and 2.5â€“9.59 mm and 7.63â€“12.88 mm for 2.5 mg/ml and 5 mg/ml of P. odoratissimus extract, respectively. Epidermophyton floccosum and Trichophyton violaceum showed the lowest MIC value of 0.15 mg/ml. The results of our study have shown that the extract from P. odoratissimus can work significantly better against fungal diseases caused by dermatophytes. It was also found that it acts as a perfect alternative to the currently available antifungals such as fluconazole and griseofulvin.

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Author Biographies

Geethavani Babu, Department of Microbiology, Bharath University, Bharath Institute of Higher Eduction and Research, Chennai, Tamil Nadu, India.

Assistant professor, Research Scholar,

Dept. Of Microbiology.

Balamuruganvelu Singaravelu, Department of Microbiology, DM Wayanad Institute of Medical Sciences, Wayanad and Bharath University, Bharath Institute of Higher Eduction and Research, Chennai, Tamil Nadu, India.

Professsor, Research scholar, Dept. of microbiology

Sreenivasalu Reddy Vallapu, Department of Microbiology, Bharath University, Bharath Institute of Higher Eduction and Research, Chennai, Tamil Nadu, India.

Professsor, Dept. of microbiology

Srikumar Ramasundaram, Department of Microbiology,Centre for Research, Sri Lakshmi Narayana Institute of Medical Sciences, Bharath University, Bharath Institute of Higher Eduction and Research, Chennai, Tamil Nadu, India.

Reserach Associate, Centre for research,

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