NEUROPROTECTIVE ANTIOXIDANT EFFECT OF PORTULACA QUADRIFIDA LINN. LEAVES EXTRACT ON IMMOBILIZATION STRESS-INDUCED CHANGES IN RAT'S BRAIN

MOAYAD JAMAL Shahwan; Sabrina Ait Gacem

doi:10.22159/ajpcr.2018.v11i10.27209

Authors

MOAYAD JAMAL Shahwan Department of Clinical Sciences, College of Pharmacy and Health Sciences, Ajman University, Ajman, United Arab Emirates.
Sabrina Ait Gacem Department of Clinical Sciences, College of Pharmacy and Health Sciences, Ajman University, Ajman, United Arab Emirates.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i10.27209

Keywords:

Antioxidant, Immobilization, Neurodegenerative diseases, Portulaca quadrifida, Stress

Abstract

Objective: The current study was established to illustrate the effect of this plant's active constituents on stress-induced diseases that include oxidation damage to cellular components, especially the brain.

Methods: Immobilization stress method is induced and initiated by putting separated animals in specially prepared mesh cages on a wooden plank. The animals were exposed to 6 h of stress. Wistar male rats were selected weighing (180â€“200 g). A total of 54 rats were selected and separated into nine groups and only six experimental rats were kept in each group.

Results: The post-stress oral treatment of extract (100 mg/kg body weight) was more efficient in restricting stress-induced decline of superoxide dismutase (p<0.05), glutathione (GSH)-S-transferase (p<0.02), catalase (p<0.05), and GSH (p<0.05) and an enhanced level of thiobarbituric acid reactive substance (p<0.01) compared to stress alone or pre-stress extract treatments.

Conclusion: The extract showed a significant resistance toward the oxidative metabolism triggered by restraint stress, though the post-extract treatment (curative) was observed to be more effective in restoring the altered oxidative metabolism compared with pre-extract treatment (prophylactic).

Downloads

Download data is not yet available.

Author Biography

MOAYAD JAMAL Shahwan, Department of Clinical Sciences, College of Pharmacy and Health Sciences, Ajman University, Ajman, United Arab Emirates.

Associate Proffesor of Clinical Biochemistry

College of Pharmacy and Health Sciences,

References

Deepika KS, Rajagopal SV. Evaluation of in vitro anticancer potential of ethanolic extract and its different fractions of Caesalpinia bonduc (L) roxb Seeds. Int J Pharm Pharm Sci 2014;20 Suppl 1:311-4.

Halliwell B. Antioxidants and human diseases: A general introduction. Nutr Rev 1997;55:44-52.

Pietta PG. Flavonoids as antioxidants. J Nat Prod 2000;63:1035-42.

Ames BN, Gold LS, Willett WC. The causes and prevention of cancer. Proc Natl Acad Sci U S A 1995;92:5258-65.

Ejiofor UE, Ebhohon SO, Nwuke PC, Atasie OC, Oriaku CE. Ameliorative effect of methanol extract of Telfairia occidentalis hook and Amaranthus hybridus Linn. against cadmium induced oxidative stress in rats. J Appl Pharm Sci 2017;9:109-15.

Sharma R, Chandan G, Chahal A. Antioxidant and anticancer activity of methanolic extract from stephania elegans. Int J Pharm Pharm Sci 2017;85 Suppl 1:245-9.

Anamarija I, Sonja MM. Polyphenolic composition and antioxidant activities of grape seed extract. Int J Food Properties 2008;11:713-26.

Fauconneau B, Waffo-Teguo P, Huguet F. Comparative study of radical scavenger and antioxidant properties of phenolic compounds from Vitis vinifera cell cultures using in vitro tests. Life Sci 1997;61:2103-10.

Ekaiko M, Arinze A, John W, Ajah O. Antimicrobial effect of the leaf extract of Psidium guajava L. and Carica papaya L. Int J Life Sci Res 2015;3:55-60.

Kirtikar KR, Basu BD. Indian Medicinal Plants. 2nd ed. Mumbai: Prakashan Publications; 2001

Hoffman BR, Delas A, Blanco K. Screening of antibacterial and antifungal activities of ten medicinal plants from Ghana. Pharm Biol 2004;42:13-7.

Mulla SK, Swamy P. Preliminary pharmacognostical and phytochemical evaluation of Portulaca quadrifida Linn. Int J Pharmtech Res 2010;2:1699-702.

Singh LK, Pang X, Alexacos N, Letourneau R, Theoharides TC. Acute immobilization stress triggers skin mast cell degranulation via corticotrophin releasing hormone neurotension and substance link to neurogenic skin disorders. Brain Behav Immunity 1999;13:225-39.

Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem 1974;47:469-74.

Beers RF Jr., Sizer IW. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase. J Biol Chem 1952;195:133-40.

Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chem 1974;249:7130-9.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951;193:265-75.

Tabrez S, Ahmad M. Effect of wastewater intake on antioxidant and marker enzymes of tissue damage in rat tissues: Implications for the use of biochemical markers. Food Chem Toxicol 2009;47:2465-78.

Tabrez S, Ahmad M. Cytochrome P450 system as a toxicity biomarker of industrial wastewater in rat tissues. Food Chem Toxicol 2010;48:998 1001.

Das M, Khanna SK. Clinicoepidemiological, toxicological, and safety evaluation studies on argemone oil. Crit Rev Toxicol 1997;27:273-97.

Levine WG. Glutathione, lipid peroxidation and regulation of cytochrome P-450 activity. Life Sci 1982;31:779-84.

Floyd RA, Carney JM. Free radical damage to protein and DNA: Mechanisms involved and relevant observations on brain undergoing oxidative stress. Ann Neurol 1992;32 Suppl 22:7.

Al-Qirim TM, Shahwan M, Zaidi KR, Uddin Q, Banu N. Effect of khat, its constituents and restraint stress on free radical metabolism of rats. J Ethnopharmacol 2002;83:245-50.

PayÃ¡ M, Halliwell B, Hoult JR. Interactions of a series of coumarins with reactive oxygen species. Scavenging of superoxide, hypochlorous acid and hydroxyl radicals. Biochem Pharmacol 1992;44:205-14.

Gallo M, Conte E, Naviglio D. Analysis and comparison of the antioxidant component of Portulaca oleracea leaves obtained by different solid-liquid extraction techniques. Antioxidants (Basel) 2017;6:64.

Mulla SK, Swamy P. Antioxidant activity of ethanolic and polyphenolic extracts of Portulaca quadrifida. Int J Biol Pharm Res 2012;3:392-9.

Uddin MK, JuraimiAS, Ali ME, Ismail MR. Evaluation of antioxidant properties and mineral composition of purslane (Portulaca oleracea L.) at different growth stages. Int J Mol Sci 2012;13:10257-67.

Verma SC, Kalyan H, Manish D, Maddi R, Biresh KS. Phytochemical and high-performance liquid chromatography analysis of extract of portulaca quadrifida linn. Asian J Pharm Clin Res 2016;9:164.