EVALUATION OF REVERSAL EFFECTS OF EUGENIA JAMBOLANA SEED EXTRACTS AGAINST HIGH-FRUCTOSE DIET-INDUCED INSULIN RESISTANCE IN ALBINO RATS

M.h.r.k Gupatha Bayya; Sachidananda Adiga Mn; Nagendra Nayak Im; Asitava Deb Roy; Sampath Kumar Ak

doi:10.22159/ajpcr.2018.v11i12.27548

Authors

M.h.r.k Gupatha Bayya Department of Pharmacology, IQ City Medical College, Durgapur, West Bengal, India.
Sachidananda Adiga Mn Department of Pharmacology, K.S. Hegde Medical Academy, NITTE (Deemed to be University), Mangalore, Karnataka, India.
Nagendra Nayak Im Department of Pharmacology, K.S. Hegde Medical Academy, NITTE (Deemed to be University), Mangalore, Karnataka, India.
Asitava Deb Roy Department of Pathology, IQ City Medical College, Durgapur, West Bengal, India.
Sampath Kumar Ak Department of General Medicine, K.S. Hegde Medical Academy, NITTE (Deemed to be University), Mangalore, Karnataka, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i12.27548

Keywords:

Eugenia jambolana, Fructose, Insulin resistance, Hyperglycemia, Hyperlipidemia and Metformin

Abstract

Objective: The objective of this study is to evaluate the reversal effects of aqueous and ethanolic extracts of jamun seeds against high-fructose (HFr) diet-induced insulin resistance (IR) in albino rats.

Methods: Thirty male albino rats were divided into five groups (n=6), and all the group rats except normal control were provided with HFr (60% w/v) to their drinking water daily for 42 days. Group 1 and 2 served as a normal and fructose control. Groups 3, 4, and 5 were supplemented with metformin (MET 500mg/kg p.o) and aqueous and ethanolic extracts of jamun seeds (Jamun seed aqueous extract [JSAE] and Jamun seed ethanolic extract [JSEE] 1000 mg/kg of each p.o), from day 28 to day 42, respectively. Physical (body weights, food, and water intake) and biochemical (glucose, insulin, and lipid) parameters were estimated, and Homeostasis model assessment (HOMA)-IR values were calculated.

Results: HFr diet significantly (p<0.05) increased weight gain and water intake with decreased food intake in rats. HFr-fed rats exhibited a significant (p<0.05) increase in fasting glucose, insulin, and lipid levels along with increased HOMA-IR values and confirms the development of IR. Supplementation with MET, JSAE, and JSEE significantly (p<0.05) restored the physical parameters and reversed fasting glucose and lipid levels in comparison with HFr control. Whereas, only JSAE had significantly reversed the fasting insulin levels in comparison with HFr control. HOMA-IR values were significantly (p<0.05) decreased in both the extract groups of HFr-fed rats, and the results were comparable to MET.

Conclusion: Our study concludes that both aqueous and ethanolic extracts of jamun exhibit a significant reversal effect against HFr diet-induced IR, due to insulin-sensitizing actions at the target tissues.

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Author Biographies

M.h.r.k Gupatha Bayya, Department of Pharmacology, IQ City Medical College, Durgapur, West Bengal, India.

DEPARTMENT OF PHARMACOLOGY, ASSISTANT PROFESSOR,

IQ City Medical College, Durgapur-713206, West Bengal, India.

Sachidananda Adiga Mn, Department of Pharmacology, K.S. Hegde Medical Academy, NITTE (Deemed to be University), Mangalore, Karnataka, India.

Professor, Department of Pharmacology, K.S. Hegde Medical Academy. Mangalore-575018, Karnataka, India.

Nagendra Nayak Im, Department of Pharmacology, K.S. Hegde Medical Academy, NITTE (Deemed to be University), Mangalore, Karnataka, India.

Professor, Department of Pharmacology, K.S. Hegde Medical Academy, Mangalore-575018, Karnataka, India.

Asitava Deb Roy, Department of Pathology, IQ City Medical College, Durgapur, West Bengal, India.

⁴Associate Professor, Department of Pathology,

IQ City Medical College, Durgapur-713206, West Bengal, India.

Sampath Kumar Ak, Department of General Medicine, K.S. Hegde Medical Academy, NITTE (Deemed to be University), Mangalore, Karnataka, India.

Professor, Department of General Medicine, K.S. Hegde Charitable Hospital. Mangalore-575018, Karnataka, India.

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