EFFECT OF MURRAYA KOENIGII LEAVES EXTRACT ON GLUCONEOGENESIS AND GLYCOGENOLYSIS IN ISOLATED RAT HEPATOCYTES CULTURE

Rohan S Phatak; Chitra C Khanwelkar; Kailas D Datkhile; Pratik P Durgawale

doi:10.22159/ajpcr.2018.v11i11.27962

Authors

Rohan S Phatak Department of Pharmacology, Krishna Institute of Medical Sciences, Karad, Maharashtra, India.
Chitra C Khanwelkar Department of Pharmacology, Krishna Institute of Medical Sciences, Karad, Maharashtra, India.
Kailas D Datkhile Department of Molecular Biology and Genetics, Krishna Institute of Medical Sciences, Karad, Maharashtra, India.
Pratik P Durgawale Department of Molecular Biology and Genetics, Krishna Institute of Medical Sciences, Karad, Maharashtra, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i11.27962

Keywords:

Murraya koenigii, Non-insulin-dependent diabetes mellitus, Gluconeogenesis, Glycogenolysis

Abstract

Objectives: The present study was aimed to investigate the in vitro activity of Murraya koenigii extracts through various carbohydrate metabolic pathways in the isolated rat hepatocyte models.

Methods: Different doses of metformin, aqueous and methanol extracts of M. koenigii leaves were evaluated in the MTT, glucose, and glycogen content assays in the cultured in vitro rat hepatocytes.

Results: The study showed that there was a significant increase in activity with respect to the increased concentration of extracts. Slight effect was observed in the isolated rat hepatocytes culture, M. koenigii leaves extract may exert cytoprotective and hypoglycemic action.

Conclusion: It may be needed to determine the effect of ex vivo rat hepatocytes isolated from diabetic rats. Effects of the plant or isolated compounds on the genes expression of signaling pathways should be investigated in further studies.

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Author Biographies

Rohan S Phatak, Department of Pharmacology, Krishna Institute of Medical Sciences, Karad, Maharashtra, India.

Ph. D Scholar

Chitra C Khanwelkar, Department of Pharmacology, Krishna Institute of Medical Sciences, Karad, Maharashtra, India.

Professor and Head

Kailas D Datkhile, Department of Molecular Biology and Genetics, Krishna Institute of Medical Sciences, Karad, Maharashtra, India.

In-charge

Pratik P Durgawale, Department of Molecular Biology and Genetics, Krishna Institute of Medical Sciences, Karad, Maharashtra, India.

Research Officer

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