EVALUATION OF SUBCHRONIC TOXICITY OF LACTOBACILLUS PARACASEI HII03 FERMENTED MORINDA CITRIFOLIA (NONI) FRUIT JUICE

Authors

  • Chaiyavat Chaiyasut Innovation Center for Holistic Health, Nutraceuticals and Cosmeceuticals, Faculty of Pharmacy, Chiang Mai University, Chiang Mai-50200, Thailand.
  • Bhagavathi Sundaram Sivamaruthi Innovation Center for Holistic Health, Nutraceuticals and Cosmeceuticals, Faculty of Pharmacy, Chiang Mai University, Chiang Mai-50200, Thailand.
  • Yodsawee Duangjitcharoen Division of Biopharmacy, Faculty of Pharmaceutical Sciences, Burapha University, Chonburi, Thailand.
  • Periyanaina Kesika Innovation Center for Holistic Health, Nutraceuticals and Cosmeceuticals, Faculty of Pharmacy, Chiang Mai University, Chiang Mai-50200, Thailand.
  • Sasithorn Sirilun Innovation Center for Holistic Health, Nutraceuticals and Cosmeceuticals, Faculty of Pharmacy, Chiang Mai University, Chiang Mai-50200, Thailand.
  • Khontaros Chaiyasut Health Innovation Institute, Chiang Mai - 50200, Thailand.
  • Sartjin Peerajan Health Innovation Institute, Chiang Mai - 50200, Thailand.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i11.28025

Keywords:

Morinda citrifolia, Noni, Lactic acid bacteria, Subchronic Toxicity, Fermented plant juice

Abstract

Objective: The present study evaluated the subchronic toxicity of Lactobacillus paracasei mediated fermented Morinda citrifolia (Noni) juice (FMJ) using Sprague Dawley rat as a model system.

Methods: FMJ was prepared. The microbial load and pH of FMJ during fermentation were measured by a plating method and using pH meter, respectively. The healthy rats were supplemented with different doses of FMJ for 60 days. The changes in body weight of the animals were measured during FMJ intervention period. The blood and organs of the experimental rats were collected and were subjected to hematological and biochemical analysis by following standard hospital protocols, after the treatment period.

Results: After 30 days of fermentation, the pH of FMJ was reduced to 4.10 from 4.31. Lactobacillus load was increased gradually during fermentation while Bacillus spp. load decreased progressively, finally after 30 days of fermentation, no Bacillus spp., and yeast was found in FMJ. FMJ supplementation did not affect the body mass of experimental animals. The alternations in the organ weight were not associated with FMJ intervention. FMJ supplementation did not significantly affect the normal range of the selected hematological and biochemical parameters, which suggested that FMJ was not harmful to experimental rats.

Conclusion: FMJ was enriched with lactic acid bacteria, and free from pathogenic microbes. FMJ was found as safe for rodent consumption without any adverse effects. Further, studies are needed to explain the beneficial effect of L. paracasei mediated FMJ.

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References

Chaiyasut C, Sivamaruthi BS, Makhamrueang N, Peerajan S, Kesika P. A survey of consumer’ opinion about consumption and health benefits of fermented plant beverages in Thailand. Food Sci Technol Campinas 2018;38:299-309.

Peerajan S, Chaiyasut C, Sirilun S, Chaiyasut K, Kesika P, Sivamaruthi BS. Enrichment of nutritional value of Phyllanthus emblica fruit juice using the probiotic bacterium, Lactobacillus paracasei HII01 mediated fermentation. Food Sci Technol Campinas 2016;36:116-23.

Komatsuzaki N, Shima J, Kawamoto S, Momose H, Kimura T. Production of γ-aminobutyric acid (GABA) by Lactobacillus paracasei isolated from traditional fermented foods. Food Microbiol 2005;22:497 504.

Woraharn S, Lailerd N, Sivamaruthi BS, Wangcharoen W, Sirisattha S, Chaiyasut C. Screening and kinetics of glutaminase and glutamate decarboxylase producing lactic acid bacteria from fermented Thai foods. Food Sci Technol Campinas 2014;34:793-9.

Chaiyasut C, Woraharn S, Sivamaruthi BS, Kesika P, Lailerd N, Peerajan S. Lactobacillus fermentum HP3 mediated fermented Hericium erinaceus juice as a health promoting food supplement to manage diabetes mellitus. J Evid Based Integr Med 2018;23:1-9.

Wang MY, West BJ, Jensen CJ, Nowicki D, Su C, Palu AK, et al. Morinda citrifolia (Noni): A literature review and recent advances in noni research. Acta Pharmacol Sin 2002;23:1127-41.

Basar S, Uhlenhut K, Hogger P, Schone F, Westendorf J. Analgesic and anti-inflammatory activity of Morinda citrifolia L. (Noni) fruit. Phytother Res 2010;24:38-42.

Dussossoy E, Brat P, Bony E, Boudard F, Poucheret P, Mertz C, et al. Characterization, anti-oxidative and anti-inflammatory effects of Costa Rican noni juice (Morinda citrifolia L.). J Ethnopharmacol 2011;133:108-15.

Lee SY, Park SL, Hwang JT, Yi SH, Nam YD, Lim SI. Antidiabetic effect of Morinda citrifolia (Noni) fermented by cheonggukjang in KK-A(y) diabetic mice. Evid Based Complement Alternat Med 2012;2012:163280.

Gupta RK, Banerjee A, Pathak S, Sharma C, Singh N. Induction of mitochondrial mediated apoptosis by Morinda citrifolia (Noni) in human cervical cancer cells. Asian Pac J Cancer Prev 2013;14:237-42.

Huang HL, Ko CH, Yan YY, Wang CK. Antiadhesion and anti-inflammation effects of noni (Morinda citrifolia) fruit extracts on AGS cells during Helicobacter pylori infection. J Agric Food Chem 2014;62:2374-83.

Suzuki M, Tung NH, Kwofie KD, Adegle R, Amoa-Bosompem M, Sakyiamah M, et al. New anti-trypanosomal active tetracyclic iridoid isolated from Morinda lucida Benth. Bioorg Med Chem Lett 2015;25:3030-3.

Mueller M, Puttipan R, Janngeon K, Unger FM, Viernstein H, Okonogi S. Bioactivities of the Thai medicinal and edible plants C. Cajan, M. citrifolia and O. americanum. Int J Pharm Pharm Sci 2015;7:237-40.

Huang C, Wei YX, Shen MC, Tu YH, Wang CC, Huang HC. Chrysin, abundant in Morinda citrifolia fruit water-EtOAc extracts, combined with apigenin synergistically induced apoptosis and inhibited migration in human breast and liver cancer cells. J Agric Food Chem 2016;64:4235-45.

Ali M, Mruthunjaya KB, Nandini CA, Nabeel KA, Manjula SN. Chemoprotective effect of noni (Morinda citrifolia L.) fruit juice against cisplatin-induced nephrotoxicity. Int J Pharm Pharm Sci 2016;8:105 10.

Mauliku NE, Hendro W, Saputro SH, Kristina TN. Anti-tubercular activity of extract and compounds of noni (Morinda citrifolia Linn). Int J Pharm Pharm Sci 2017;9:105-9.

Singh D. Morinda citrifolia L. (Noni): A review of the scientific validation for its nutritional and therapeutic properties. J Diabetes Endocrinol 2012;3:77-91.

Millonig G, Stadlmann S, Vogel W. Herbal hepatotoxicity: Acute hepatitis caused by a noni preparation (Morinda citrifolia). Eur J Gastroenterol Hepatol 2005;17:445-7.

Stadlbauer V, Weiss S, Payer F, Stauber RE. Herbal does not at all mean innocuous: The sixth case of hepatotoxicity associated with Morinda citrifolia (noni). Am J Gastroenterol 2008;103:2406-7.

West BJ, Su CX, Jensen J. Prenatal toxicity test of Morinda citrifolia (noni) fruit. J Toxicol Sci 2008;33:647-9.

Rosly SM, Shanmugavelu S, Murugaiyah M, Hadijah H, Tarmizi SA, Noridayusni Y, et al. Subchronic oral toxicity study of Morinda citrifolia (Mengkudu) in Sprague Dawley rats. Pertanika J Trop Agric Sci 2011;34:341-9.

Lagarto A, Bueno V, Merino N, Piloto J, Valdés O, Aparicio G, et al. Safety evaluation of Morinda citrifolia (noni) leaves extract: Assessment of genotoxicity, oral short term and subchronic toxicity. J Intercult Ethnopharmacol 2013;2:15-22.

West BJ. Report of Morinda citrifolia chronic toxicity not applicable to commercial noni juice. Regul Toxicol Pharmacol 2017;88:360-1.

Abu N, Zamberi NR, Yeap SK, Nordin N, Mohamad NE, Romli MF, et al. Subchronic toxicity, immunoregulation and anti-breast tumor effect of nordamnacantal, an anthraquinone extracted from the stems of Morinda citrifolia L. BMC Complement Altern Med 2018;18:31.

Woraharn S, Lailerd N, Sivamaruthi BS, Wangcharoen W, Peerajan S, Sirisattha S, et al. Development of fermented Hericium erinaceus juice with high content of L-glutamine and L-glutamic acid. Int J Food Sci Technol 2015;50:2104-12.

de Sousa BC, Miguel CB, Rodrigues WF, Machado JR, da Silva MV, da Costa TA, et al. Effects of short-term consumption of Morinda citrifolia (Noni) fruit juice on mice intestine, liver and kidney immune modulation. Food Agric Immunol 2017;28:528-42.

Westendorf J, Effenberger K, Iznaguen H, Basar S. Toxicological and analytical investigations of noni (Morinda citrifolia) fruit juice. J Agric Food Chem 2007;55:529-37.

Zahi AK, Hamzah H, Hutheyfa S, Shaari MR, Sithambaram S, Othman HH. Acute and sub-acute dermal toxicity studies of Morinda citrifolia L. fruit extract in Sprague Dawley rats. Asian J Pharm Clin Res 2015;8:400-8.

West BJ, Jensen CJ, Westendorf J. Noni juice is not hepatotoxic. World J Gastroenterol 2006;12:3616-9.

West BJ, Su CX, Jensen J. Hepatotoxicity and subchronic toxicity tests of Morinda citrifolia (noni) fruit. J Toxicol Sci 2009;34:581-5.

Published

07-11-2018

How to Cite

Chaiyasut, C., B. S. Sivamaruthi, Y. Duangjitcharoen, P. Kesika, S. Sirilun, K. Chaiyasut, and S. Peerajan. “EVALUATION OF SUBCHRONIC TOXICITY OF LACTOBACILLUS PARACASEI HII03 FERMENTED MORINDA CITRIFOLIA (NONI) FRUIT JUICE”. Asian Journal of Pharmaceutical and Clinical Research, vol. 11, no. 11, Nov. 2018, pp. 381-4, doi:10.22159/ajpcr.2018.v11i11.28025.

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