MORINDA COREIA HAS AN ANTI-HELICOBACTER PYLORI EFFECT AGAINST THE MULTIDRUG-RESISTANT CLINICAL ISOLATE OF NORTH-EAST INDIA

Shweta Mahant; Valentina Gehlot; Shanu Hoda; Pooja Vijayaraghavan; Selvamurthy W; Thirumurthy N; Rajashree Das

doi:10.22159/ajpcr.2018.v11i12.28064

Authors

Shweta Mahant Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India.
Valentina Gehlot Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India.
Shanu Hoda Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India.
Pooja Vijayaraghavan Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India.
Selvamurthy W Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India.
Thirumurthy N BT Herbal, Sivakasi, Tamil Nadu, India.
Rajashree Das Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i12.28064

Keywords:

Helicobacter pylori, Morinda coreia, Resistance, Antibiogram

Abstract

Objective: Antimicrobial resistance is a growing problem in Helicobacter pylori eradication which is a microaerophilic bacterium causing various gastroduodenal diseases. The present study has been designed to test the efficacy of Morinda coreia against the metronidazole clarithromycin and levofloxacin-resistant H. pylori strains isolated from the biopsy taken from the patient suffering from gastric erosion in Guwahati, Assam.

Method: The antimicrobial activity of n-hexane and chloroform extract of M. coreia was tested against multidrug-resistant H. pylori isolate of Guwahati, Assam, by agar well method and microdilution method.

Result: In the present study, the H. pylori strain resistant for metronidazole (minimal inhibitory concentration [MIC] >64 Î¼g/mL), clarithromycin at (MIC >0.5 Î¼g/mL), and levofloxacin at (MIC >1 Î¼g/mL) was tested against the n-hexane and chloroform extract of M. coreia. Both the extracts of M. coreia showed good efficacy against the multidrug-resistant strain of H. pylori shown inhibition at 1.2 Î¼g/mL with n-hexane extract and 2 Î¼g/mL with chloroform extract of M. coreia.

Conclusion: The prevalence of metronidazole-resistant ranges between 50% and 90% in the developing countries, including India, clarithromycin ranges from 0% to 15% in India, and levofloxacin ranges between 50% and 70% in India, so there is a need of alternative therapy for the eradication of this bacterium from the stomach. Hence, this study suggests that M. coreia, which has been used traditionally as a folk medicine for the treatment of many gastric diseases, has also shown good efficacy against the multidrug-resistant H. pylori strain of North-east India.

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Author Biographies

Shweta Mahant, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India.

Assistant Professor, Amity Institute of Biotechnology, Amity University, Noida.

Valentina Gehlot, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India.

Ph. D scholar, Amity Institute of Biotechnology, Amity University, Noida.

Shanu Hoda, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India.

Professor, Amity Institute of Biotechnology, Amity University, Noida.

Pooja Vijayaraghavan, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India.

Present, Amity Science, Technology & Innovation Foundation, Amity University, Noida, Uttar Pradesh

Selvamurthy W, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India.

BT Herbal, Sivakasi, Tamil Nadu

Thirumurthy N, BT Herbal, Sivakasi, Tamil Nadu, India.

Research Assistant, Amity Institute of Biotechnology, Amity University, Noida.

Rajashree Das, Amity Institute of Biotechnology, Amity University, Noida, Uttar Pradesh, India.

Associate Professor, Amity Institute of Biotechnology, Amity University, Noida.

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