P-SELECTIN GLYCOPROTEIN LIGAND-1 GENE POLYMORPHISMS IN SUDANESE PREGNANT WOMEN WITH THROMBOSIS

Abdelrahman A Elhassan; Fathelrahman Mahdi Hassan; Hanan B Eltahir

doi:10.22159/ajpcr.2018.v11i12.28200

Authors

Abdelrahman A Elhassan Department of Hematology, College of Medical Laboratory Science, Sudan University of Science and Technology, Khartoum, Sudan.
Fathelrahman Mahdi Hassan Department of Clinical Laboratory Science, College of Applied Medical Science, Imam Abdulrahman Bin Faisal University, Saudi Arabia.
Hanan B Eltahir Department of Biochemistry, Faculty of Medicine and Health Sciences, University of El Imam El Mahadi, Sudan.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i12.28200

Keywords:

P-selectin glycoprotein ligand-1 polymorphisms, Variable number of tandem repeats, Thrombosis, Deep vein thrombosis

Abstract

Objectives: In this study we aimed to investigate whether P-selectin gene polymorphism is associated with thrombosis in Sudanese pregnant women in Khartoum state.

Methods: After informed consent, 96 Sudanese pregnant women with thrombosis and 53 healthy pregnant women were recruited in the study. Genomic DNA was isolated from whole blood. Genotyping of PSGL-1 gene was performed by polymerase chain reaction amplification for exon 14 and electrophoresis.

Results: The frequency of the B allele was found to be significantly higher in pregnant women with thrombosis (26%) compared to the controls (17.4%).

Conclusion: The AB genotype was found to be higher in women with thrombosis 40.6% than in healthy controls 20% (p=0.02). Our results suggest that the PSGL-1 AB genotype is associated with an increased risk of thrombosis in Sudanese pregnant women.

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Author Biography

Abdelrahman A Elhassan, Department of Hematology, College of Medical Laboratory Science, Sudan University of Science and Technology, Khartoum, Sudan.

Hematologist, Medical Laboratory specialist, Hemostasis, and hematology lecturer.

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