ASSESSMENT OF ANTIOXIDATIVE AND AMELIORATIVE POTENTIAL OF AQUEOUS EXTRACT OF LEAVES OF THUJA ORIENTALIS (PLATYCLADUS ORIENTALIS [L.])
DOI:
https://doi.org/10.22159/ajpcr.2018.v11i12.28205Keywords:
Thuja, Chlorpyrifos, Antioxidant, GenoprotectiveAbstract
Objective: The main objective of the present study is to assess the antioxidative and attenuating potential of aqueous extract of leaves of Thuja orientalis (AET) against the genotoxicity induced by chlorpyrifos (CPF) (O,O-diethyl O-(3,5,6-trichloro-2-pyridyl) phosphorothioate).
Methods: Phytochemical analysis of the extract was performed to reveal the presence of various bioactive compounds. Free radical scavenging activity was assessed using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay and further genoprotective activity of AET was evaluated by in vivo studies using rat as model. For this purpose, male healthy rats, 8–10 weeks old, weighing 120±10 g, were selected and divided into three groups, namely Group 1 fed with corn oil, taken as control, Group 2 fed with CPF, and Group 3 treated with AET 1 week before CPF exposure. ¼ of LD50, i.e., 38 mg/kg CPF was used for experiment and was orally administered to rats.
Results: The presence of bioactive compounds such as saponins, alkaloids, flavonoids, and terpenes was confirmed by phytochemical analysis. DPPH assay revealed the IC50 value which was deduced to be 205.04 μl/ml. In vivo study including exposure to pesticide alone for 24 h, 48 h, and 72 h showed significant increase in DNA damage (p≤0.01) in liver, kidney, and blood as compared to control. However, a dose of AET 400 mg/kg p.o. given 1 week before each duration of CPF exhibited significant protection from CPF-induced genotoxicity.
Conclusion: Thus, the study suggests the positive effect of aqueous leaf extract of T. orientalis in ameliorating the damage induced by pesticide exposure.
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Zama D, Meraihi Z, Tebibel S, Benayssa W, Benayache F, Benayache S, et al. Chlorpyrifos-induced oxidative stress and tissue damage in the liver, kidney, brain and fetus in pregnant rats: The protective role of the butanolic extract of Paronychia argentea. Indian J Pharm 2007;39:145 50.
Khan MS, Yusufzai SK, Ying LY, Zulnashriq W. GC-MS based chemical profiling and evaluation of antioxidant potential of leaves and stems of Alternanthera sessilis red from Sabah, Malaysia. Int J Pharm Pharm Sci 2018;10:4-9.
Balakrishnan BB, Krishnasamy K. Evaluation of free radical screening and antioxidant potential of Moringa concanensis nimmo-a medicinal plant used in Indian traditional medication system. Int J Pharm Pharm Sci 2018;10:91-7.
Fierascu I, Ungureanu C, Avramescu SM, Cimpeanu C, Georgescu MI, Fierascu RC, et al. Genoprotective, antioxidant, antifungal and anti inflammatory evaluation of hydroalcoholic extract of wild-growing Juniperus communis L. (Cupressaceae) native to Romanian Southern Sub-Carpathian hills. BMC Complement Altern Med 2018;18:3.
Durzan DJ. Arginine, scurvy and cartier’s tree of lifeâ€. J Ethnobiol Ethnomed 2009;5:5.
Tsiri D, Graikou K, Pobłocka-Olech L, Krauze-Baranowska M, Spyropoulos C, Chinou I, et al. Chemosystematic value of the essential oil composition of Thuja species cultivated in Poland-antimicrobial activity. Molecules 2009;14:4707-15.
Chang LC, Song LL, Park EJ, Luyengi L, Lee KJ, Farnsworth NR, et al. Bioactive constituents of Thuja occidentalis. J Nat Prod 2000;63:1235 8.
Naser B, Bodinet C, Tegtmeier M, Lindequist U. Thuja occidentalis (Arbor vitae): A Review of its pharmaceutical, pharmacological and clinical properties. Evid Based Complement Alternat Med 2005;2:69 78.
Biswas R, Mandal SK, Dutta S, Bhattacharyya SS, Naoual B, KhudaBukhsh AR. Thujone-rich fraction of Thuja occidentalis demonstrates major anti-cancer potentials: Evidences from in vitro. Evid Based Complement Alternat Med 2011;568148:16.
Dubey SK, Batra A. Hepatoprotective activity from ethanol fraction of Thuja occidentalis Linn. Asian J Res Chem 2008;1:32-5.
Yogesh K, Ali J. Antioxidant potential of Thuja (Thuja occidentalis) cones and peach (Prunus persia) seeds in raw chicken ground meat during refrigerated (4±1 °C) storage. J Food Sci Technol 2014;51:1547 53.
Sah SN, Regmi S, Tamang M. Antibacterial effects of Thuja leaves extract. Int J Appl Sci Biotechnol 2017;5:256-60.
Sunila ES, Kuttan G. Protective effect of Thuja occidentalis against radiation-induced toxicity in mice. Integr Cancer Ther 2005;4:322-8.
Ansaruddin PA, Vijayalakshmi K. The womb is not safe anymore. Ind Agr News 2003;2:3.
Čolović MB, Vasić VM, Avramović NS, Gajić MM, Djurić DM, Krstić DZ, et al. In vitro evaluation of neurotoxicity potential and oxidative stress responses of diazinon and its degradation products in rat brain synaptosomes. Toxicol Lett 2015;233:29-37.
Malla FA. Assessment of total protein concentration in liver of fresh water fish, Channa punctuates (bloch) with special reference to an organophosphate insecticide, chlorpyrifos. Int J Pharma Bio Sci 2012;3:B567-71.
Ali D, Nagpure NS, Kumar S, Kumar R, Kushwaha B. Genotoxicity assessment of acute exposure of chlorpyrifos to freshwater fish Channa punctatus (Bloch) using micronucleus assay and alkaline single-cell gel electrophoresis. Chemosphere 2008;71:1823-31.
Saunders M, Magnanti BL, Correia Carreira S, Yang A, Alamo- Hernández U, Riojas-Rodriguez H, et al. Chlorpyrifos and neurodevelopmental effects: A literature review and expert elicitation on research and policy. Environ Health 2012;11 Suppl 1:S5.
Olusola A, Olutayo O, Michael A, Olakunle F, Edah AO. Elemental analysis and anti-microbial potentials of the leaf extract of Cassia arereh. Int Res J Pharm Pharm 2011;1:188-93.
Brand-Williams A, Cuvelier ME, Berset C. Use of a free radical method to evaluate antioxidant activity. Lebensm-Wiss. u.-Technol 1995;28:25 30.
Sharma S, Chadha P. Induction of neurotoxicity by organophosphate pesticide chlorpyrifos and modulating role of cow urine. Springerplus 2016;5:1344.
Bedi JS, Gill JP, Aulakh RS, Kaur P, Sharma A, Pooni PA, et al. Pesticide residues in human breast milk: Risk assessment for infants from Punjab, India. Sci Total Environ 2013;463-464:720-6.
Jack D, Thrasher PD, Madison R, Broughton A. Immunologic abnormalities in humans exposed to chlorpyrifos: Preliminary observations. Arch Environ Health 2010;45:89-93.
Umosen AJ, Ambali SF, Ayo JO, Mohammed B, Uchendu C. Alleviating effects of melatonin on oxidative changes in the testes and pituitary glands evoked by subacute chlorpyrifos administration in wistar rats. Asian Pac J Trop Biomed 2012;2:645-50.
Roszczenko A, Rogalska J, Moniuszko-Jakoniuk J, Brzóska MM. The effect of exposure to chlorfenvinphos on lipid metabolism and apoptotic and necrotic cells death in the brain of rats. Exp Toxicol Pathol 2013;65:531-9.
Mansour SA, Mossa HT. Lipid peroxidation and oxidative stress in rat erythrocytes induced by chlorpyrifos and the protective effect of zinc. Pest Biochem Physiol 2009;93:34-9.
Mukherjee A, Sikdar S, Khuda-Bukhsh AR. Evaluation of ameliorative potential of isolated flavonol fractions from Thuja occidentalis in lung cancer cells and in Benzo(a)pyrene induced lung toxicity in mice. J Tradit Complement Med 2016;1:1-13.
Daryoush HA, Malihe AS, Mohadjerani M, Ferns GA, Amir A. Prooxidant-antioxidant balance and antioxidant properties of Thuja orientalis L: A potential therapeutic approach for diabetes mellitus. Curr Mol Pharmacol 2018;11:109-12.
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