IN VITRO EVALUATION OF THE ANTHELMINTIC ACTIVITY OF RHIZOME EXTRACTS OF CURCUMA LONGA (LINN.)

Jyoti Pandey; Suman Mishra; Kamal Jaiswal

doi:10.22159/ajpcr.2018.v11i12.28313

Authors

Jyoti Pandey Department of Zoology, Babasaheb Bhimrao Ambedkar University, Lucknow, Uttar Pradesh, India.
Suman Mishra Department of Zoology, Babasaheb Bhimrao Ambedkar University, Lucknow, Uttar Pradesh, India.
Kamal Jaiswal Department of Zoology, Babasaheb Bhimrao Ambedkar University, Lucknow, Uttar Pradesh, India.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11i12.28313

Keywords:

Anthelmintic activity, Curcuma longa, Nematodiasis, Haemonchus spp, Albendazole

Abstract

Objective: The current study was carried out to evaluate the anthelmintic activity of the rhizome extract of Curcuma longa as an alternative source of effective remedies for nematodiasis.

Methods: The anthelmintic activity of the C. longa was assessed in vitro against Haemonchus spp., a gastrointestinal (abomasum) parasite of goats. Different concentrations of the extracts (1 mg/mL, 2.5 mg/mL, 5 mg/mL, and 10 mg/mL) in phosphate-buffered saline (PBS) were tested, and the results expressed in terms of time of paralysis (minute) and time of death (minute) of the worms. Albendazole (1 mg/mL) was used as a reference (positive control) and PBS as a control group (negative control).

Results: The qualitative phytochemical analysis of the methanolic extract (ME) of the plant disclosed the presence of alkaloids, glycosides, terpenoids, flavonoids, tannins, saponins, phenol, anthraquinone, and carbohydrates; whereas, the aqueous extract (AE) showed the presence of alkaloids, carbohydrate, flavonoids, and saponins. Both ME and AE of the C. longa (rhizome) expressed significant efficacy (pâ‰¤0.05) in causing paralysis as well as the death of the worms within 12 h of exposure at all tested concentrations, as compared to the negative control. The rhizome extracts of C. longa showed dose-dependent efficacy in causing paralysis of the worm motility and the final progression to death. The results showed that the ME at 10 mg/mL was significantly more potent (pâ‰¤0.05) over the AE.

Conclusions: This study concluded that the rhizome extract of C. longa exhibited potent anthelmintic efficacy against the nematode parasite, Haemonchus spp.

Downloads

Download data is not yet available.

Author Biography

Jyoti Pandey, Department of Zoology, Babasaheb Bhimrao Ambedkar University, Lucknow, Uttar Pradesh, India.

department of Zoology

References

Perry BD, Randolph TF, McDermott JJ, Sones KR, Thornton PK. Investing in Animal Health Research to Alleviate Poverty. Nairobi: International Livestock Research Institute; 2002. p. 148.

Katoch R, Mandial RK, Nagal KB. Outbreak of Haemonchus contortus infection in sheep of Himachal Pradesh. Indian Vet J 1999;76:932-3.

Kaur H, Kaur D. Prevalence of gastrointestinal parasites in domestic animals of Patiala and its adjoining areas. J Vet Parasitol 2008;22:25-8.

Yadav CL, Rajeev RK, Banerjee PS, Godara R, Garg R, Vatsya S. Epidemiology of gastro-intestinal nematodes in sheep. Indian Vet J 2009;86:1010-3.

Coop RL, Holms PH. Nutrition and parasite interaction. Intern J Parasitol 1996;26:951-62.

Githigia SM, Thamsborg SM, Munyua WK, Maingi N. Impact of gastrointestinal helminths on production in goats in Kenya. Small Rumin Res 2001;42:21-9.

Chaturvedi M, Dwivedi S, Dwivedi A, Barpete PK, Sachan R. Formulation and evaluation of polyherbal anthelmintic preparation. Ethnobot Leaflet 2009;13:329-31.

Trease GE, Evans WC. A Textbook of Pharmacognosy. 12th ed. London: Bailliere Tindal Ltd.; 1989. p. 374-726.

Thompson P, Geary TG. The Structure and Function of Helminth Surfaces. In: Biochemistry and Molecular Biology of Parasites. 1th ed. New York: Academic Press; 1995. p. 203-32.

Akhtar MS, Zafar I, Khan MN, Muhammad L. Anthelmintic activity of medicinal plants with particular reference to their use in animals in Indo-Pakistan subcontinent. Small Rumin Res 2000;8:99-107.

Singh R, Mehta A, Mehta P, Shukla K. Anthelmintic activity of rhizome extracts of Curcuma longa and Zingiber officinale (Zingiberaceae). Int J Pharm Pharm Sci 2011;3:236-7.

Maheshwari RK, Singh AK, Gaddipati J, Srimal RC. Multiple biological activities of curcumin: A short review. Life Sci 2006;78:2081-7.

Akram M, Udin S, Ahmed A,Usmanghani K, Hanan A, Mohiudin E, et al. Curcuma longa and curcumin: A review article. Rom J Biol Plant Biol 2010;5:65-70.

Morais ER, Oliveira KC, Magalhaes LG, Moreira EB, Verjovski- Almeida S, RodriguesV. Effects of curcumin on the parasite Schistosoma mansoni: A transcriptomic approach. Mol Biochem Parasitol 2013;187:91-7.

Bazh EK, El-BahyNM. In vitro and in vivo screening of anthelmintic activity of ginger and curcumin on Ascaridia galli. Parasitol Res 2013;112:3679-86.

Ullah S, Khan MN, Sajid MS, Muhammad G. Comparative anthelmintic efficacy of Curcuma longa, Citrullus colocynthis and Peganum harmala. Glob Vet 2013;11:560-7.

Ullah R, Rehman A, Zafeer F, Rehman L, Khan YA, Khan MA, et al. Anthelmintic potential of thymoquinone and curcumin on Fasciola gigantica. PLoS One 2017;12:e0171267.

Sermakkani M, Thangapandian V. Phytochemical screening for active compounds in Pedalium murex. Recent Res Sci Tech 2010;2:110-4.

Khyade M, Kasote D, Kshirsagar S, Gaikwad J, Bharati K. Anthelmintic screening of some plants used in traditional medicine. Asian J Biomed Pharma Sci 2012;2: 516-22.

Harborne JB. Phytochemical Methods. A Guide to Modern Techniques of Plant Analysis. London, New York: Chapman and Hall Ltd.; 1973. p. 279.

Sofowora A. Medical Plants and Traditional Medicine in Africa. 2nd ed. Ibadan, Nigeria: Spectrum Books Ltd.; 1993. p. 71-3.

Wang JF, Zhao LW, Li J. In vivo anthelmintic activity of five alkaloids from Macleaya microcarpa (Maxim) fedde against Dactylogyrus intermedius in Carassius auratus.Vet Parasitol 2010;171:305-13.

Hegde K, Joshi AB. Scholars research library. Der Pharm Lett 2010;2:255.

Sawant RS, Godghate AG. Qualitative phytochemical screening of rhizomes of Curcuma longa Linn. Int J Sci Environ Technol 2013:2;634-41.

Dash GK, Suresh P, Kar DM, Ganpaty S, Panda SB. Evaluation of evolvulus alsinoids linn for anthelmintic and antimicrobial activities. J Nat Rem 2002;2:182-5.

Eguale T, Tilahun G, Debella A, Feleke A, Makonnen E. Haemonchus contortus: In vitro and in vivo anthelmintic activity of aqueous and hydro-alcoholic extracts of Hedera helix. Exper Parasito 2007;116:340-5.

Iqbal Z, Nadeem QK, Khan MN, Akhtar MS, Waraich FN. In vitro anthelmintic activity of Allium sativum, Zingiber officinale, Curcurbita mexicana and Ficus religiosa. Int J Agric Biol 2001;3:454-7.

Ghosh T, Maity TK, Bose A, Dash GK. Anthelmintic activity of Bacopa monierri. Indian J Nat Prodct 2005;21:16-19.

Swargiary A, Nath P, Basumatary B, Brahma D. Phytochemical, antioxidant, and trace element analysis of anthelmintic plants of North- East India. Int J Pharm Pharm Sci 2017;9:228-32.

Parvathy NG, Padma R, Renjith V, Kalpana PR, Saranya TS. Phytochemical screening and anthelmintic activity of methanolic extract Ofimperata cylindrica. In J Pharma Pharma Sci 2012;4: 232-4.

Kumar AK, chandrashekar KR. Phytochemical evaluation and in vitro antimicrobial and antioxidant studies of leaf and stem bark extracts of Polyalthia fragrans (dalz) bedd-an endemic species of Western Ghats. Int J Pharm Pharm Sci 2017:9;20-4.

Anthnasiadou S, Kyriazakis I, Jackson F, Coop RL. Direct anthelmintic effects of condensed tannins towards different gastrointestinal nematodes of sheep: In vitro and in vivo studies. Vet Parasitol 2000;99:205-19.

Srivastav R, Pathak V, Tripathi IP. Comparative phytochemical and physicochemical study of tulsi (Ocimum sanctum) and haldi (Curcuma longa). In J Pharm Bio Arch 2018;9:55-7.

Roy H, Chakroborty A, Bhanja S, Nayak BS, Mishra SR, Ellaiah P. Preliminary phytochemical investigation and anthelmintic activity of Acanthospermum hispidum DC. J Pharma Sci Tech 2010;2:217-21.

Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and extraction: A review. Pharm Sci 2011;1:98-106.

Martin RJ. Modes of action of antihelmentic drugs. Vet J 1997;154:11-34.

Rajesh H, Rao SN, Rani NM, Shetty PK, Rejeesh EP, Chandrashekar R. Phytochemical analysis of methanolic extract of Curcuma longa linn rhizome. In J Uni Pharm Bio Sci 2013;2: 39-45.

Kane SR, Mohite SK, Shete JS. Antihelmintic activity of aqueous and methanolic extracts of Euphorbia thymifolia Linn. In J Pharm Tech Res 2009;1:666-9.

De S, Dey A, Babu AM, Aneela S. In vitro anthelmintic activity of aqueous and methanolic extracts of Oldenlandia umbellate. Int J Pharm Pharm Sci 2014;6:94-5.

Iqbal Z, Sarwar M, Jabbar A, Ahmed S, Nisa M, Sajid MS et al. Direct and indirect anthelmintic effects of condensed tannins in sheep. Vet Parasito 2007;144:125-31.

Bharathi NM, Sravanthi V, Sujeeth S, Kalpana K, Santhoshi. P, et al. In-vitro anthelmintic activity of methanolic and aqueous extracts of Achyranthes aspera Linn. (Amaranthaceae) Stems. J Pharm Sci 2013;3:181-4.

Bashir AL, Chishti MZ, Bhat FA, Tak H, Bandh SA, Khan A. Anthelmintic activities of aqueous and methanol extracts of Prunella vulgaris L. Nat Prod Chem Res 2017;5:4.

Debella A. Manual for Phytochemical Screening of Medicinal Plants. Addis Ababa, Ethiopia: Ethiopian Health and Nutrition Research Institute; 2002.