ZN(II)/AU(I) AND ZN(II)/AG(I) COMPLEXES WITH SALEN SCHIFF BASE EXPRESS PROMISING CYTOTOXIC ACTIVITY IN HUMAN CANCER CELLS

Authors

  • Tanya Zhivkova Department of , Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Sofia, Bulgaria.
  • Gabriela Marinescu Department of, Institute of Physical Chemistry Ilie Murgulescu,” Romanian Academy, Bucharest, Romania.
  • Lora Dyakova Department of , Institute of Neurobiology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
  • Daniela Cristina Culita Department of, Institute of Physical Chemistry Ilie Murgulescu,” Romanian Academy, Bucharest, Romania.
  • Rossen Spasov Department of , Faculty of Medicine, Sofia University St. Kliment Ohridski,” Sofia, Bulgaria.
  • Pavel Mitrenga BB - NCIPD Ltd., Sofia, Bulgaria.
  • Reni Kalfin Department of , Institute of Neurobiology, Bulgarian Academy of Sciences, Sofia, Bulgaria.
  • Luminita Patron Department of, Institute of Physical Chemistry Ilie Murgulescu,” Romanian Academy, Bucharest, Romania.
  • Radostina Alexandrova Department of , Institute of Experimental Morphology, Pathology and Anthropology with Museum, Bulgarian Academy of Sciences, Sofia, Bulgaria.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i1.28403

Keywords:

Cytotoxic activity, Human cancer cell lines, H2Salen, Schiff bases, Zn(II)Au(I), Zn(II)Ag(I) complexes

Abstract

Objective: The aim of our study was to evaluate the influence of two complexes of Zn(II)/Au(I) and Zn(II)/Ag(I) with Schiff base ligand (H2Salen) obtained from the condensation reaction between salicylaldehyde and ethylenediamine (abbreviated ZnSalenAu, ZnSalenAg) on viability and proliferation of cultured human cancer cells.

Methods: The following cell lines were used as model systems: Human cervical carcinoma (cervical carcinoma), A549 (non-small cell lung cancer [NSCLC]), glioblastoma multiforme (8MGBA), and A431 (squamous cell carcinoma) and its multidrug-resistant (MDR) clones A431-MDR, A431-MRP, and A431-ABCG2 that express mdr1, mrp1, or abcg2 gene, respectively. The investigations were performed by thiazolyl blue tetrazolium bromide test, neutral red uptake cytotoxicity assay, crystal violet staining, hematoxylin and eosin staining, double staining with acridine orange, and propidium iodide in short-term experiments (12–72 h, with monolayer cell cultures) as well as colony-forming method in long-term experiments (25 days, with three dimensional cancer cell colonies).

Results: The results obtained revealed that ZnSalenAu and ZnSalenAg decreased significantly viability and proliferation of the treated cells in a time- and concentration-dependent manner being more active as compared to the free ligand H2Salen.

Conclusion: The present study demonstrates for the 1st time the ability of two heterometallic complexes ZnSalenAu and ZnSalenAg to decrease significantly viability and proliferation of cultured cell lines established from some of the most common and aggressive human cancers (NSCLC, carcinoma of uterine cancer, 8MGBA, and squamous cell carcinoma) as well as MDR cancer cells.

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Published

07-01-2019

How to Cite

Zhivkova, T., G. Marinescu, L. Dyakova, D. Cristina Culita, R. Spasov, P. Mitrenga, R. Kalfin, L. Patron, and R. Alexandrova. “ZN(II)/AU(I) AND ZN(II)/AG(I) COMPLEXES WITH SALEN SCHIFF BASE EXPRESS PROMISING CYTOTOXIC ACTIVITY IN HUMAN CANCER CELLS”. Asian Journal of Pharmaceutical and Clinical Research, vol. 12, no. 1, Jan. 2019, pp. 458-64, doi:10.22159/ajpcr.2019.v12i1.28403.

Issue

Vol 12 Issue 1 January 2019

Section

Original Article(s)

The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.

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