ANTI-INFLAMMATORY ACTIVITY OF METHANOLIC EXTRACT OF HIBISCUS PLANTIFOLIUS
DOI:
https://doi.org/10.22159/ajpcr.2018.v11i11.28429Keywords:
Hibiscus plantifolius, Anti-inflammatory, SoxhletionAbstract
Objective: The objective of the present study was to carry out anti-inflammatory activity of methanolic extract of Hibiscus plantifolius (MEHP) belonging to the family Malvaceae.
Methods: The shade dried stem part of H. plantifolius (1 kg) was powdered and extracted with methanol using soxhletion. The extract was concentrated using rotary evaporator under reduced pressure at 40°C, till free from the solvents and thereby providing crude methanol extract which was subsequently employed for further studies. Anti-inflammatory effect was studied by carrageenan-induced paw edema model in rats at dose level of 50, 150, and 300 mg/kg. Acute oral toxicity study was also studied.
Results: The results indicate that MEHP, 300 mg/kg, exhibited significant inhibition (p<0.001) of increase in paw edema at 4th h.
Conclusion: The results of the experimental study confirmed that MEHP possesses significant anti-inflammatory activity.
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References
Rodan GA. Introduction to bone biology. Bone 1992;13 Suppl 1:S3-6.
Ojewole JA. Antinociceptive, anti-inflammatory and antidiabetic effects of Bryophyllum pinnatum (Crassulaceae) leaf aqueous extract. J Ethnopharmacol 2005;99:13-9.
Akindahunsi AA, Olaleye MT. Toxicological investigation of aqueous-methanolic extract of the calyces of Hibiscus sabdariffa L. J Ethnopharmacol 2003;89:161-4.
Trusheva B, Trunkova D, Bankova V. Different extraction methods of biologically active components from propolis: A preliminary study. Chem Cent J 2007;1:13.
Rafik U, Mahesh MS, Rajesh NP. Evaluation of anti-inflammatory activity of selected medicinal plants used in Indian traditional medication system in vitro as well as in vivo. J Tradit Complement Med 2016;6:355-61.
Al-Saghir J, Al-Ashi R, Salloum R, Saliba NA, Talhouk RS, Homaidan FR, et al. Anti-inflammatory properties of salograviolide a purified from Lebanese plant Centaurea ainetensis. BMC Complement Altern Med 2009;9:36.
Tambewagh UU, Kandhare AD, Honmore VS, Kadam PP, Khedkar VM, Bodhankar SL, et al. Anti-inflammatory and antioxidant potential of guaianolide isolated from Cyathocline purpurea: Role of COX-2 inhibition. Int Immunopharmacol 2017;52:110-8.
Antonisamy P, Agastian P, Kang CW, Kim NS, Kim JH. Anti-inflammatory activity of rhein isolated from the flowers of Cassia fistula L. and possible underlying mechanisms. Saudi J Biol Sci 2017;2017:30.
Lee SH, Soyoola E, Chanmugam P, Hart S, Sun W, Zhong H, et al. Selective expression of mitogen-inducible cyclooxygenase in macrophages stimulated with lipopolysaccharide. J Biol Chem 1992;267:25934-8.
Reed RK, Westerberg EJ. Effect of alpha-trinositol on carrageenan-induced rat paw edema and lowering of interstitial fluid pressure. Eur J Pharmacol 1999;376:279-84.
Ravi V, Saleem TS, Patel SS, Raamamurthy J, Gauthaman K. Anti-inflammatory effect of methanolic extract of Solanum nigrum Linn Berries. Int J Appl Res Nat Prod 2009;2:33-6.
Mahat MA, Patil BM. Evaluation of anti-inflammatory activity of methanolic extracts of Phyllanthus amarus in experimental animal models. Indian J Pharm Sci 2007;4:33-5.
Desouky SK, Gamal-Eldeen AM. Cytotoxic and anti-inflammatory activities of some constituents from the floral buds of Syringa patula. Pharm Biol 2009;47:872-7.
Gupta M, Mazumder UK, Gomathi P, Selvan VT. Anti-inflammatory evaluation of leaves of Plumeria acuminata. BMC Complement Altern Med 2006;6:36.
Kyei S, Koffuor GA, Boampong JN. The efficacy of aqueous and ethanolic leaf extracts of Pistia stratiotes Linn. In the management of arthritis and fever. J Med Biomed Sci 2012;1:29-37.
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