ANTICANCER ACTIVITY OF MICRO-ALGAE EXTRACT ON HUMAN CANCER CELL LINE (MG-63)
DOI:
https://doi.org/10.22159/ajpcr.2019.v12i1.28652Keywords:
Microalgae, Anticancer, MG-63 cell line, 3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay, Gas chromatography-mass spectrometry, PolyphenolAbstract
Objective: The objective of the present study is to assess the anticancer effect of limnic algae extract of KACC 2 (Karunya Algae Culture Collection) and KACC 23 against MG-63 cell line.
Methods: Bioactive compounds were extracted from the algae culture by Soxhlet extraction method using methanol as solvent, and the extract was checked for its anticancer activity. Polyphenols were estimated using Folin-Ciocalteu reagent method. The constituents of the extract were analyzed using gas chromatography-mass spectrometry (GC-MS).
Results: The results showed that KACC 2 had more phenolic content than KACC 23. The half maximal inhibitory concentration value for KACC 2 and 23 was found as 0.386 μl/ml and 0.285 μl/ml of extracts necessary for the 50% of cell death. GC-MS analysis revealed that the compounds with possible therapeutic effects are quercetin, stigmast-4-en-3-one, epoxygedunin, which has got anticancer activity.
Conclusion: These observations point out that the algae extract of KACC 2 and KACC 23 has anticancer property and the phytoconstituents contributed to the anticancer effects.
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