IN VITRO EVALUATION OF ANTIOXIDANT-MEDIATED APOPTOSIS ON CANCER CELLS AND NON-TOXICITY OF ETHANOLIC EXTRACT OF TERMINALIA CHEBULA RETZ AND PYROGALLOL
DOI:
https://doi.org/10.22159/ajpcr.2019.v12i1.28667Keywords:
Terminalia chebula, Antioxidant, Apoptosis, HemolysisAbstract
Objective: Antioxidants are very important for human health, and hence, external supplementation is recommended to protect cells from the deleterious effects of excessive reactive oxygen species conditions that are capable of causing direct damage to deoxyribonuclieic acid (DNA) which might lead to the cause of cancer. In our research work, we have aimed to evaluate the antioxidant potential of Terminalia chebula and pyrogallol to relate to its apoptotic nature as well as prove its non-toxicity.
Methods: Investigations were carried out to determine the antioxidant activity by 2,2-diphenyl-1-picrylhydrazyl and lipid peroxidation inhibition assay of the ethanolic extract of T. chebula and pyrogallol. The apoptotic nature of tumor cells was determined by comet assay. To prove the non-toxicity of the extract as well as compound, the hemolysis assay was carried out.
Results: The results of our study established the antioxidant potential of the plant which may serve as additional evidence for the antiproliferative activity of the plant. It is considered safe for human use.
Conclusion: Plant-based traditional medicine system continues to play an essential role in health care. The ethanolic extract of T. chebula and pyrogallol possesses antioxidant and anticancer properties.
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