ISOLATION AND IDENTIFICATION OF NON-POLAR CHEMICAL ENTITY FROM LEPTADENIA RETICULATA AERIAL PARTS
DOI:
https://doi.org/10.22159/ajpcr.2019.v12i2.28683Keywords:
Leptadenia reticulata, Jivanti, Hentriacontane, Triterpenoid, SterolAbstract
Objective: Leptadenia reticulata is the medicinal plant having many biological activities. It is necessary to find out which types of phytochemical constituents are present in the plant. The objective of this investigation was to isolate and identify the non-polar chemical entity of the areal parts of L. reticulata a plant used for medicinal purpose in folklore.
Methods: Petroleum ether extract of the stem bark was used for this study. Non-polar solvent was used to extract non-polar chemical entity from areal parts of leptadeniareticulata. Through the saponification process, saponifiable and unsaponifiable matter was separated. Phytochemical constituents were separated using column chromatography. Separated fractions were analyzed on gas chromatography and mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR).
Results: Hentriacontane compound was isolated and confirmed from GC-MS and NMR whereas phytol, Lupeol, β-amyrin, Campesterol, Stigmasterol, gamma-sitosterol identified through GC and mass spectroscopy.
Conclusion: The present study showed that L. reticulata areal parts three sterols chemical entity Campesterol, Stigmasterol, gamma-sitosterol and phytol (diterpene alcohol), lupeol (triterpenoid), beta-amyrin (triterpene), and hentriacontane (alkane hydrocarbon). Core determination of the experiment was the development efficient method to isolate or identify the non-polar chemical entity through chromatographic technique.
Downloads
References
Kasera PK, Shukla JK. Bio-medicinal properties and cultivation of L. reticulata - an endengered plant of thar desert, India. Scientific correspondance. Curr Sci 2003;84:877-9.
Naik R, Harisha CR, Acharya R. A comparative pharmacognostical evaluation of three botanical source plants of jivanti. Int J Pharm Pharm Sci 2018;10:61-6.
Sivarajan V, Balachandran I. Ayurvedic Drugs and Their Plant Sources. New Delhi: Oxford and IBH; 1999. p. 195-200.
Dhalani JM, Nariya PB. A pharmacological review: Leptadenia reticulata (wight and arn.); Jivanti: The real life giving plant. Folia Med 2017;59:405-12.
Mohanty SK, Swamy MK, Sinniah UR, Anuradha M. Leptadenia reticulata (Retz.) wight and arn. (Jivanti). Botanical, agronomical, phytochemical, pharmacological, and biotechnological aspects. Molecules 2017;22:1-27.
Pal A, Sharma PP, Pandya TN, Acharya R, Patel BR, Shukla VJ, et al. Phyto-chemical evaluation of dried aqueous extract of jivanti Leptadenia reticulata (Retz.) wt. Et arn. Ayu 2012;33:557-60.
Hilditch TP, Williams PN. The Chemical Composition of Natural Fats. 4th ed. New York: John Wiley and Sons, Inc.; 1964. p. 90.
James AT, Morris LJ. New Biochemical Separations. London: Van Nastrond; 1964. p. 321.
Kaufmann HP. Analyse der Fette and Kettprodukte. Berlin: Springer; 1958. p. 1302-751.
Aslan MA, Iimutdin MA. The study of microalgae Nannochloropsis salina fatty acid composition of the extracts using different techniques, SCF vs conventional extraction. J Mole Liq 2017;239:96-100.
Nariya PB, Shukla VJ, Acharya RN, Nariya MB, Bhatt PV, Pandit CM, et al. Isolation and characterization of phytosterols from Cordia macleodii bark by chromatographic and spectroscopic method. Asian J Pharm Clin Res 2014;7:86-8.
Baker DA, Ibrahim E, Ahmed K, Farouk K. Sterols bioactivity of Ruta graveolens L. and Murraya paniculata L. Int J Pharm Pharm Sci 2017;9:103-8.
Sutar R, Kasture S, Kalaichelvan V. Isolation and identification of a new phytosterol from Holoptelea integrifolia (ROXB) planch leaves. Int J Pharm Pharm Sci 2014;6:354-7.
Dhalani J, Kapadiya K, Pandya M, Dubal G, Imbraj P, Nariya P. An approach to identify sterol entities from Abrus precatorius’s seeds by GC-MS. J Sci Indu Res 2018;77:297-300.
Goad LJ, Akihisa T. Analysis of Sterols. 1st ed. London, UK: Blackie Academic and Professional, Chapman and Hall; 1997.
Published
How to Cite
Issue
Section
The publication is licensed under CC By and is open access. Copyright is with author and allowed to retain publishing rights without restrictions.
