OPTIMIZATION OF GROWTH AND BIOACTIVE METABOLITE PRODUCTION: FUSARIUM SOLANI.
Abstract
The present study aimed to evaluate optimize the growth and antibacterial activity of endophytic fungi isolated from Tylophora indica. Based on the morphological and molecular characteristic, the isolate was identified as Fusarium solani. Modified liquid medium (M2D) was used as a basal medium for growth and antibacterial activity. The growth and metabolite production were optimized with 4% dextrose, 0.05% yeast extract and aspartic acid (0.01%) as carbon, nitrogen and amino acid respectively. The optimum pH and temperature of the strain were 6.0, 25±2 °C observed for growth and secondary metabolite production. The 9th day was found to be optimum incubation period of growth and secondary metabolite production. The metabolite showed maximum inhibition against Enterococcus faecalis, lowest inhibition zone was against Yersinia enterocolitica. The ESI-MS analysis of bio active compound shows the peak at 301m/z.
Key words:
Endophytic fungus, Tylophora indica, antibacterial activity, Medium Optimization, ESI -MS
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