• Vinoy Jacob Department of Biotechnology, Karpagam Academy of Higher Education, Eachanari, Coimbatore, Tamil Nadu, India.
  • Rajiv P Department of Biotechnology, Karpagam Academy of Higher Education, Eachanari, Coimbatore, Tamil Nadu, India.



Green synthesis, Zinc oxide nanoparticles, Rhizome, Curcuma longa, Antibacterial, Antifungal, Antioxidant


Objective: Curcuma longa is a known natural medicine for inflammation from ancient times. It has a low absorption rate and poor solubility. Hence, it is used for the green synthesis of nanoparticles. Zinc oxide nanoparticle (ZnO NPs) is famous nanoparticles which are economical, less toxic, and brilliantly biocompatible. They have potential biomedical properties, mainly anticancer, antidiabetic, and antimicrobial.

Methods: The present study was designed to investigate in vitro analysis of the antimicrobial activity against pathogenic bacteria and fungi and its ability to scavenge reactive oxygen radicals of ZnO NPs.

Results and Conclusion: The results indicated that ZnO NPs produced from C. longa had higher antimicrobial activity against Escherichia coli, Staphylococcus aureus, Streptococcus pyogenes, and Candida albicans. Therefore, we suggest that ZnO NPs can be used as the antimicrobial agent. It is a good scavenger of superoxide radical, nitric oxide, and hydrogen peroxide and has reducing power, which is greater than ascorbic acid at a higher concentration.


Download data is not yet available.


Zhao J, Castranova V. Toxicology of nanomaterials used in nanomedicine. J Toxicol Environ Health B Crit Rev 2011;14:593-632.

Jayanta S, Avik M, Santhos K. A novel green synthesis of silver nanoparticles and their catalytic action in reduction of methylene blue dye. Sust Environ Res 2017;27:245-50.

Sunita P, Rajeshwari S, Rajiv P, Rajendran V, Seenivasan R. Green synthesis of silver nanoparticle from leaf extract of Aegle marmelos and evaluation of its antibacterial activity. Int J Pharm Pharm Sci 2015;7:169-73.

Kuppusamy P, Yusoff MM, Maniam GP, Govindan N. Biosynthesis of metallic nanoparticles using plant derivatives and their new avenues in pharmacological applications - An updated report. Saudi Pharm J 2016;24:473-84.

Agarwal H, Kumar SV, Rajeshkumar S. A review on green synthesis of zinc oxide nanoparticles – An eco-friendly approach. Res Eff Tech 2017;3:406-13.

Jamdagni P, Khatri P, Rana JS. Green synthesis of zinc oxide nanoparticles using flower extract of nyctanthes arbor-tristis and their antifungal activity. J King Saud Univ 2018;30:168-75.

Rajiv P, Rajeshwari S, Venckatesh R. Bio-fabrication of zinc oxide nanoparticles using leaf extract of Parthenium hysterophorus L. And its size-dependent antifungal activity against plant fungal pathogens. Spectrochim Acta A Mol Biomol Spectrosc 2013;112:384-7.

Narendhran S, Rajiv P, Rajeshwari S. Influence of zinc oxide nanoparticles on growth of Sesamum Indicum L. In zinc deficient soil. Int J Pharm Pharm Sci 2016;8:365-71.

Murali M, et al. Antibacterial and antioxidant properties of biosynthesized zinc oxide nanoparticles from Ceropegia candelabrum L. An endemic species. Spectrochim Acta A Mol Biomol Spectrosc 2017;15:104-9.

Nagajyothi PC, Sang Ju, Yang IJ, Sreekanth TV, Kim KJ, Shin HM. Antioxidant and anti-inflammatory activities of zinc oxide nanoparticles synthesized using Polygala tenuifolia root extract. J Photochem Photobiol B 2015;146:10-7.

Suresha D, Nethravathi PC, Udayabhanu CG, Rajanaik H, Nagabhushana H, Sharma SC. Green synthesis of multifunctional zinc oxide (ZnO) nanoparticles using Cassia fistula plant extract and their photodegradative, antioxidant and antibacterial activities. Mat Sci Semicon Proc 2015;3:446-54.

Balaji S, Kumar MB. Facile green synthesis of zinc oxide nanoparticles by Eucalyptus globulus and their photocatalytic and antioxidant activity. Adv Powder Technol 2017;28:785-97.

Sawant VJ, Bamane SR, Pachchapurkar SM. Chitosan encapsulated curcumin loaded zinc ferrite core shell nanoassembly for biocompatible drug delivery on chicken embryonic stem cells. Der Chemica Sinica 2013;4:67-78.

Fatimah I, Septian PY, Lintang MN. Green Synthesis of ZnO Nanoparticles via Complex Formation by using Curcuma longa extract. AIP Conference Proceedings 2016.

Raghad DH, Abdul J, Rasha S, Ahmed N, Abd N. Biological synthesis of titanium dioxide nanoparticles by Curcuma longa plant extract and study its biological properties. WSN 2016;49:204-22.

Shameli K, Ahmad MB, Zamanian A, Sangpour P, Shabanzadeh P, Abdollahi Y, et al. Green biosynthesis of silver nanoparticles using curcuma longa tuber powder. Int J Nanomedicine 2012;7:5603-10.

Rajasekar P, Priyardharshini S, Rajarajeshwari T, Shivashri C. Bio-inspired synthesis of silver nanoparticles using Andrographis paniculata whole plant extract and their anti-microbial activity over pathogenic microbes. Int J Res Biomed Biotech 2013;3:4752-61.

Magaldi S, Mata S, Hartung C. In vitro susceptibility to fluconazole of Candida spp. isolates comparing three different methods. J Mycol Med 2001;11:123-6.

Malterud KE, Farbrot TL, Huse AE, Sund RB. Antioxidant and radical scavenging effects of anthroquinones and anthrones. Pharmacological 1993;47:77-85.

Gocer HY, Menzek A, Gulcin I. Synthesis and antioxidantproperties of (3,4.dihydroxyphenyl) (2,3,4-trihydroxyphenyl) methanoneand its derivaties. Arch Pharm (Weinheim) 2012;345:323-4.

Oyaizu M. Studies on product of browning reaction, antioxidative activities of product of browning reaction prepared from glucosamine. Jpn J Nutr 1986;44:307-15.

Nabavi SM, Ebrahimzedh MA, Navadi SF, Bahmaneslami MF. In vitro antioxidant and free radical scavenging activity of Diospyros lotus and Pyrus boissieriana growing in Iran. Pharm Mag 2009;4:122-6.

Nishikimi M, Rao NA, Yagi K. The occurance of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochem Biopsy Res Commun 1972;46:849-54.

Rasmussen JW, Martinez E, Louka P, Wingett DG. Zinc oxide nanoparticles for selective destruction of tumor cells and potential for drug delivery applications. Expert Opin Drug Deliv 2010;7:1063-77.

Xiong HM. ZnO nanoparticles applied to bioimaging and drug delivery. Adv Mater 2013;25:5329-35.

Zhang ZY, Xiong HM. Photoluminescent ZnO nanoparticles and their biological applications. Materials 2015;8:3101-27.

Shi LE, Li ZH, Zheng W, Zhao YF, Jin YF, Tang ZX, et al. Synthesis, antibacterial activity, antibacterial mechanism and food applications of znO nanoparticles: A review. Food Addit Contam Part A Chem Anal Control Expo Risk Assess 2014;31:173-86.

Jiang Y, Zhang L, Wen D, Ding Y. Role of physical and chemical interactions in the antibacterial behavior of znO nanoparticles against E. Coli. Mater Sci Eng C Mater Biol Appl 2016;69:1361-6.

Dutta RK, Nenavathu BP, Gangishetty MK, Reddy AV. Antibacterial effect of chronic exposure of low concentration znO nanoparticles on E. Coli. J Environ Sci Health A Tox Hazard Subst Environ Eng 2013;48:871-8.

Ghannoum MA, Rice LB. Antifungal agents: Mode of action, mechanism of resistance, and correlation of these mechanisms with bacterial resistance. Clin Microbiol Rev 1999;12:501-17.

Nucci M, Marr KA. Emerging fungal diseases. Clin Infect Dis 2005;41:521-6.

Newman DJ, Cragg CM. Natural products as sources of new drugs over the last 25 years. J Nat Prod 2007;70:461-77.

Bhakya S, Muthukrishnan S, Sukumaran M, Muthukumar M. Biogenic synthesis of silver nanoparticles and their antioxidant and antibacterial activity. Appl Nanosci 2015;10:1-12.

Contreras-Guzman ES, Strong FC. Determination of tocopherols (Vitamin E) by reduction of cupricion. J AOAC Int 1982;65:1215-7.

Huang D, Ou B, Prior RL. The chemistry behind antioxidant capacity assays. J Agric Food Chem 2005;53:1841-56.

Schaffazick SR, Pohlmann AR, de Cordova CA, Creczynski-Pasa TB, Guterres SS. Protective properties of melatonin-loaded nanoparticles against lipid peroxidation. Int J Pharm 2005;289:209-13.

Hema H. Hypoglycemic, hypolipidemic and antioxidant properties of combination of curcumin from Curcuma longa Linn and partially purified product from Abromaugusta Linn in streptozotocin induced diabetes. Indian J Clin Biochem 2002;17:33-43.

Halliwel B, Gutteridge JMC. Free Radicals in Biology and Medicine. New York: Oxford University Press; 1999.

Yen GC, Duh PC, Tsai CL. Relationship between antioxidant activity and maturity of peanut hulls. J Agric Food Chem 1993;41:67-70.

Taylor BS, Kim YM, Wang Q, Shapiro RA, Billiar TR, Geller DA, et al. Nitric oxide down-regulates hepatocyte-inducible nitric oxide synthase gene expression. Arch Surg 1997;132:1177-83.

Robak J, Gryglewsk RJ. Flavonoids are scavenger of superoxide anions. Bio Chem Pharm 1988;37:837-41.



How to Cite

Jacob, V., and R. P. “IN VITRO ANALYSIS: THE ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF ZINC OXIDE NANOPARTICLES FROM CURCUMA LONGA”. Asian Journal of Pharmaceutical and Clinical Research, vol. 12, no. 1, Jan. 2019, pp. 200-4, doi:10.22159/ajpcr.2019.v12i1.28808.



Original Article(s)