A NEW REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR THE SIMULTANEOUS ESTIMATION OF SERRATIOPEPTIDASE AND DICLOFENAC SODIUM IN BULK AND TABLET DOSAGE FORM

Manasi Kulkarni B; Anagha Joshi M

doi:10.22159/ajpcr.2019.v12i1.28857

Authors

Manasi Kulkarni B PhD Research Scholar, Department of Pharmaceutical Sciences, PRIST University, Thanjavur, Tamil Nadu, India.
Anagha Joshi M Principal, SCESâ€™s Indira College of Pharmacy, Tathawade, Pune, Maharashtra, India.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i1.28857

Keywords:

Serratiopeptidase, Diclofenac sodium, Methanol, Acetonitrile, Phosphate buffer pH-7, O-phosphoric acid, Reversed-phase highperformance liquid chromatography

Abstract

Objective: The objective is to study the development of a simple, rapid, specific, precise, and accurate reversed-phase high-performance liquid chromatography (RP-HPLC) method for the simultaneous estimation of serratiopeptidase (SER) and diclofenac (DC) sodium in bulk and tablet formulation.

Methods: RP-HPLC method was developed for the simultaneous estimation of SER and DC sodium in tablet formulation. The separation was achieved by Kromasil C18 column (250 mm Ã— 4.6 mm, 5 Î¼m particle size) with phosphate buffer pH-7 and o-phosphoric acid:methanol:acetonitrile (5:4:1% v/v/v). Flow rate was maintained at 1 mL/min and UV detection was carried at 270 nm.

Result: For RP-HPLC method, the retention time for SER and DC sodium was found to be 3.3833 min and 8.1667 min, respectively. The method was validated for accuracy, precision, and specificity. Linearity for SER and DC sodium was in the range of 5â€“50 Î¼g/ml.

Conclusion: The developed RP-HPLC method is simple, accurate, rapid, sensitive, precise, and economic. Hence, this method can be employed successfully for the estimation of SER and DC sodium in both bulk and tablet dosage forms.

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