PHYTOCHEMICAL SCREENING, ANTIMICROBIAL, AND ANTIOXIDANT ACTIVITIES OF ROOT AND LEAF EXTRACTS OF LEUCAS ASPERA
DOI:
https://doi.org/10.22159/ajpcr.2019.v12i3.29085Keywords:
Leucas aspera, Antimicrobial activity, Antioxidant activity, Fourier transform infrared analysisAbstract
Objective: The present study was designed to evaluate the phytochemical screening, antimicrobial, and antioxidant activities from the roots and leaves of Leucas aspera.
Methods: The phytochemical screening and Fourier transform infrared (FTIR) analysis of root and leaf extracts were studied using standard methods. The disk diffusion method was performed to analyze the antimicrobial activity of aqueous extract, methanol extract and hexane extract of root and leaf against to selected bacterial and fungal strains. Antibiotics, streptomycin and nystatin were used as standards for bacteria and fungi, respectively. The antioxidant activity of the extracts was evaluated by 1,1-diphenyl-2-picrylhydrazyl (DPPH) and scavenging of H2O2 free radicals.
Results: Preliminary phytochemical screening of extracts exhibited the presence of carbohydrates, proteins, amino acids, steroids flavonoids, terpenoids, saponins, alkaloids, tannins, and phlobatannins. Leaf extracts exhibited effective antibacterial and antifungal activities compared with root extracts against all the tested bacteria and fungi. ME of the leaves exhibited highest antibacterial activity against Staphylococcus aureus (12.8±0.31 mm), followed by Bacillus subtilis (11.4±0.3 mm), Escherichia coli (9.8±0.21 mm), and Pseudomonas aeruginosa (7.3±0.29 mm). Leaf extracts of L. aspera showed effective scavenging activity compared with root extracts. ME of the leaves showed maximum scavenging activities of 38.39 and 36.85%, respectively, against DPPH and H2O2 free radicals with half maximal inhibitory concentration values of 136.17 and 142.42 μg/ml.
Conclusion: Phytochemical analysis and FTIR spectrum revealed that different plant secondary metabolites particularly alkaloids, terpenoids, and flavonoids could be responsible for antimicrobial and antioxidant activities of L. aspera leaf extracts.
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Hamil FA, Apiob S, Mubirub NK, Zirabac RB, Mosangoc M, Maganyic OW, et al. Traditional herbal drugs of Southern Uganda, II: Literature analysis and antimicrobial assays. J Ethnopharm 2003;84:57-78.
Barbour EK, Al Sharif M, Sagherian VK, Habre AN, Talhouk RS, Talhouk SN, et al. Screening of selected indigenous plants of Lebanon for antimicrobial activity. J Ethnopharmacol 2004;93:1-7.
Gislene GF, Locatelli J, Freitas CP, Silva LG. Antibacterial activity of plant extracts and phytochemicals on antibiotic resistant bacteria. Br J Microbiol 2000;31:247-56.
Ríos JL, Recio MC. Medicinal plants and antimicrobial activity. J Ethnopharmacol 2005;100:80-4.
Dabur R, Gupta A, Mandal TK, Singh DD, Bajpai V, Gurav AM, et al. Antimicrobial activity of some Indian medicinal plants. Afr J Tradit Complement Altern Med 2007;4:313-8.
Selvamohan T, Ramadas V, Kishore SS. Antimicrobial activity of selected medicinal plants against some selected human pathogenic bacteria. Adv App Sci Res 2012;3:3374-81.
Amenu D. Antimicrobial activity of medicinal plant extracts and their synergistic effect on some selected pathogens. Am J Ethnomed 2014;1l:18-29.
Javid T, Adnan M, Tariq A, Akhtar B. Antimicrobial activity of three medicinal plants (Artemisia indica, Medicago falcata and Tecoma stans). Afr J Tradit Complement Altern Med 2015;12:91-6.
Sulieman AM, Shaarawy SM, Alghamdi AA. Evaluation of antimicrobial and synergistic effects of selected medicinal plants of Hail area with antibiotics. Biosci Biotech Res Comm 2017;10:44-50.
Shahidi F. Natural Antioxidants Chemistry, Health Effects and Applications. 8th ed. Champaign: AOCS Press; 1997. p. 414.
Duduku K, Nithyanandam R, Sarbatly R. Phytochemical constituents and activities of Morinda citrifolia L. and Universiti Malaysia Sabah. In: Phytochemicals – A Global Perspective of Their Role in Nutrition and Health. Ch. 6. Malaysia: InTech; 2012. p. 127-50.
Zhang YJ, Gan RY, Li S, Zhou Y, Li AN, Xu DP, et al. Antioxidant phytochemicals for the prevention and treatment of chronic diseases. Molecules 2015;20:21138-56.
Panche AN, Diwan AD, Chandra SR. Flavonoids: An overview. J Nutri sci 2016;47:1-15.
Mandal SM, Dey S. LC-MALDI-TOF MS-based rapid identification of phenolic acids. J Biomol Tech 2008;19:116-21.
Sasidharan S, Chen Y, Saravanan D, Sundram KM, Yoga Latha L. Extraction, isolation and characterization of bioactive compounds from plants’ extracts. Afr J Tradit Complement Altern Med 2011;8:1-0.
Thamizhanban D, Rani TG, Pravalika P. A review on hyphenated separation techniques used in pharmaceutical analysis. IOSR J Pharm Bio Sci 2016;11:65-74.
Prajapati MS, Patel JB, Modi K, Shah MB. Leucas aspera: A review. Pharmacogn Rev 2010;4:85-7.
Karthikeyan S, Sivakumar A, Anbalagan M, Nalini E, Gothandam KM. Finger printing of alkaloids, steroids and flavonoids using HPTLC of Leucas aspera L. Whole plant methanolic extract. J Pharm Sci Res 2013;5:67-71.
Chouhan SH, Singh KS. A review of plants of genus Leucas. J Pharm Phytothe 2011; 3(3): 13-26.
Vijay kumar G, Devanna N. An Update of Leucas aspera– A medicinal plant (Review). Int J Sci Res Med Human 2016;5:485-503.
Harborne JB. Phytochemical Methods, A Guide to Modern Techniques of Plant Analysis. 2nd ed. New York: Chapman and Hall; 1998.
Ramamurthy N, Kannan S. Fourier transform infrared spectroscopic analysis of a plant (Calotropis gigantea Linn) from an industrial village, Cuddalore Dt, Tamilnadu, India. Rom J Biophys 2007;17:269.
Cruickshank R. Medical Microbiology: A Guide to Diagnosis and Control of Infection. Edinburgh: E & S Livingston Ltd.; 1968.
Netala VR, Kotakadi VS, Bobbu P, Gaddam SA, Tartte V. Endophytic fungal isolate mediated biosynthesis of silver nanoparticles and their free radical scavenging activity and anti microbial studies 3 Biotech 2016;6:132.
Rammohan A, Gunasekar D, Reddy NV, Vijaya T, Devillee A, Bodo B, et al. Structure elucidation and antioxidant activity of the phenolic compounds from rhynchosia suaveolens. Nat Prod Commun 2015;10:609-11.
Patel A, Patel A, Patel A, Patel NM. Determination of polyphenols and free radical scavenging activity of tephrosia purpurea linn leaves (Leguminosae). Pharmacognosy Res 2010;2:152-8.
Mangathayaru K, Thirumurugan D, Patel PS, Pratap DV, David DJ, Karthikeyan J. Isolation and identification of nicotine from Leucas aspera (wild) Lin. Int J Pharm Sci 2006;68:88-90.
Das K, Das B, Arpita FK, Morshed MA, Uddin A, Bhattacherjee R, et al. Phytochemical screening and antioxidant activity of Leucas aspera. Int J Pharm Sci Res 2011;2:1746-52.
Gupta N, Subhramanyam EV, Sharma RK. Antidiabetic and hypoglycaemic activity of the crude extracts of the plant Leucas aspera. Int J Phar Inn 2011;1:1-9.
Ilango K, Ramya S, Gopinath G. Antibacterial activity of Leucas aspera spreng. Int J Chem Sci 2008;6:526-30.
Sengul M, Yildiz H, Gungor N, Cetin B, Eser Z, Ercisli S, et al. Total phenolic content, antioxidant and antimicrobial activities of some medicinal plants. Pak J Pharm Sci 2009;22:102-6.
Karimi E, Oskoueian E, Hendra R, Oskoueian A, Jaafar HZ. Phenolic compounds characterization and biological activities of citrus aurantium bloom. Molecules 2012;17:1203-18.
Khacha-ananda S, Tragoolpua K, Chantawannakul P, Tragoolpua Y. Antioxidant and anti-cancer cell proliferation activity of propolis extracts from two extraction methods. Asian Pac J Cancer Prev 2013;14:6991-5.
Pourmorad F, Hosseinimehr SJ, Shahabimajd N. Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants. Afr J Biotech 2006;11:1142-5.
Capelari-Oliveira P, Paula CA, Rezende SA, Campos FT, Grabe- Guimarães A, Lombardi JA, et al. Anti-inflammatory activity of Lychnophora passerina, Asteraceae (Brazilian “Arnica”). J Ethnopharmacol 2011;135:393-8.
Rathee P, Chaudhary H, Rathee S, Rathee D, Kumar V, Kohli K, et al. Mechanism of action of flavonoids as anti-inflammatory agents: A review. Inflamm Allergy Drug Targets 2009;8:229-35.
Lin Y, Shi R, Wang X, Shen HM. Luteolin, a flavonoid with potential for cancer prevention and therapy. Curr Cancer Drug Targets 2008;8:634-46.
Tahareen S, Shwetha R, Myrene RD. Potential antioxidant, anti-inflammatory and antibacterial evaluation of extracts of Leucas aspera using in vitro model. Int J Pharm Pharm Sci 2016;8:292-7.
Chew AL, Jessica JJ, Sasidharan S. Antioxidant and antibacterial activity of different parts of Leucas aspera. Asian Pac J Trop Biomed 2012;2:176-80.
Nagarasan S, Boominathan M. Perspective pharmacological activities of Leucas aspera: An indigenous plant species. Indo Am J Pharm Res 2016;6:6567-72.
Akter M, Khan MA, Muhsin MD, Hamid K, Ullah MO, Bulbul IJ, et al. In vitro studies on antibacterial, antifungal, and cytotoxic properties of Leucas aspera. Bio Med 2012;4:183-7.
Netala VR, Ghosh SB, Bobbu PL, Dandu A, Tartte V. Triterpenoid saponins: A review on biosynthesis, applications and mechanism of their action. Int J Pharm Pharm Sci 2015;7:24-8.
Tschesche R. Advances in the chemistry of antibiotics substances from higher plants Pharm Phytochem 1970;3:274-89.
Molyneux P. The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol 2004;26:211-9.
Moon J, Schibamoto T. Antioxidant assays for plant and food components. J Agri Food Chem 2009;57:1655-66.
Aruoma OI, Cuppett SL. Antioxidant Methodology In Vivo and In Vitro Concepts. Champaign: AOCS Press; 1997. p. 41-172.
Sowndhararajan K, Kang SC. Free radical scavenging activity from different extracts of leaves of bauhinia vahlii wight and arn. Saudi J Biol Sci 2013;20:319-25.
Benabdesselam FM, Khentache S, Bougoffa K, Chibane M. Antioxidant activities of alkaloid extracts of two Algerian species of Fumaria: Fumaria capreolata and Fumaria bastardii. Rec Adv Nat Prod 2007;1:28-35.
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