EVALUATION AND COMPARISON OF CYTOTOXIC EFFECT OF VILAZODONE HYDROCHLORIDE WITH 5-FLUOROURACIL IN HT-29 BOWEL CANCER CELL LINE

LATHA PRIYA A; ANUSHA D; DARLING CHELLATHAI K; HEMALATHA A; JEGAN MOHAN Y

doi:10.22159/ajpcr.2019.v12i2.29097

Authors

LATHA PRIYA A Department of Pharmacology, Sri Ramachandra Medical College and Research Institute, Porur, Chennai - 600 116, Tamil Nadu, India.
ANUSHA D Department of Pharmacology, Sri Ramachandra Medical College and Research Institute, Porur, Chennai - 600 116, Tamil Nadu, India.
DARLING CHELLATHAI K Department of Pharmacology, Sri Ramachandra Medical College and Research Institute, Porur, Chennai - 600 116, Tamil Nadu, India.
HEMALATHA A Department of Pharmacology, Karpagam Faculty of Medical Sciences and Research, Coimbatore, Tamil Nadu, India.
JEGAN MOHAN Y Department of General Medicine, Shri Sathya Sai Medical College and Research Institute, Ammapettai, Tamil Nadu, India.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i2.29097

Keywords:

Vilazodone hydrochloride, Colon cancer cell line, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, Selective serotonin reuptake inhibitor

Abstract

Objectives: Vilazodone hydrochloride is a novel selective serotonin reuptake inhibitor (SSRI) used to treat major depressive disorders. There are only sparse data available to know about the SSRI’s and its association with colon cancer. This study aims to evaluate and compare the in vitro cytotoxic effect of vilazodone with 5-fluorouracil (5-FU) in HT-29 cell line.

Methods: Cell viability was tested by the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay (Mosmann, 1983). Test sample and standard drug in variable concentrations were added to the HT-29 cell lines for incubation over 24 h under ideal conditions. After washing the test and standard drug sample from the well with saline, MTT was added and incubated for 4 h. Dimethyl sulfoxide of 1 ml was added in all wells after incubation with MTT. The absorbance at 570 nm was measured with an ultraviolet - spectrophotometer.

Results: The values were tabulated, and the graph was plotted to find the IC-50 value (inhibitory concentration at 50%) which was struck at 28.5 μg/ml and12. 8 μg/ml for vilazodone hydrochloride and 5-FU, respectively.

Conclusion: The results show that vilazodone hydrochloride has good anticancer property comparable with 5-FU, which would probably play a role as a cytotoxic agent in tumor cells. The proposed mechanism of action could be by activation of caspase-3 enzyme, thereby increasing apoptosis and indicates its use in coexisting depression and colon carcinoma. Other mechanism includes suppression of oncogene p53, which can be confirmed by future studies.

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Author Biographies

LATHA PRIYA A, Department of Pharmacology, Sri Ramachandra Medical College and Research Institute, Porur, Chennai - 600 116, Tamil Nadu, India.

3rd year post-graduate, M.D.,Pharmacology, Department of pharmacology, Sri Ramachandra Medical college & research institute, Porur, Chennai.

Ranked as Grade -A in NAAC Grading

ANUSHA D, Department of Pharmacology, Sri Ramachandra Medical College and Research Institute, Porur, Chennai - 600 116, Tamil Nadu, India.

Associate professor, Department of pharmacology, Sri Ramachandra Medical college & research institute, Porur, Chennai.

Ranked as Grade -A in NAAC Grading

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