EVALUATION OF HEMATINIC ACTIVITY OF NAGA BHASMA IN PHENYLHYDRAZINE-INDUCED ANEMIA IN EXPERIMENTAL RATS

YADAV AV; UNDALE VR

doi:10.22159/ajpcr.2019.v12i2.29256

Authors

YADAV AV Department of Pharmacology, MCE Society’s Allana College of Pharmacy, Pune, Maharashtra, India.
UNDALE VR Department of Pharmacology, Dr. D. Y. Patil Institute of Pharmaceutical Sciences and Research, Pune, Maharashtra, India.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i2.29256

Keywords:

Naga bhasma, Anemia, Phenylhydrazine

Abstract

Objective: Bhasmas are biologically produced nanoparticles prescribed with several other medicines of Ayurveda. Metal-based drugs are prepared by transmutation of base metals into noble ones along with the use of plant extracts meant to eradicate the toxic effects of metal. Naga bhasma (NB) is one of such metallic preparation used in various diseases such as diarrhea, spleen enlargement, and diabetes. The present study aims to test the hematinic activity of NB against phenylhydrazine (PHZ)-induced anemia in rats.

Methods: The experiment was carried on Wistar rats of either sex (150–200 g). Anemia was induced by an oral administration of PHZ 40 mg/kg for a period of 2 days. Hemoglobin (Hb) was measured on the 3rd day. Rats with Hb <11 g/dl were selected for the study. The animals were divided into six groups containing six animals each. Group I served as normal control, Group II as anemic control, and Group III was considered as standard that received ferrous sulfate (100 mg/kg), while Groups IV, V, and VI were treated with test NB 100, 200, and 400 mg/kg, respectively, for 15 days. Hb was checked on the 0, 2nd, 7th, and 15th days.

Results: Oral administration of PHZ decreased Hb from 14 g/dl at day 0 to 11 g/dl at day 2. NB induced a significant increase in Hb concentration to 13 at the dose of 100 mg/kg p.o. after 48 h.

Conclusion: PHZ decreased Hb rate inducing anemia. NB caused an increase in the Hb values in anemic rats. Thus, it can be concluded that NB exhibits significant hematinic activity in rats.

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