NARINGENIN-LOADED D-Α-TOCOPHERYL POLYETHYLENE GLYCOL SUCCINATE 1000 POLYMERIC NANOSUSPENSION: AN IN VITRO AND IN VIVO ANTI-INFLAMMATORY ACTIVITY

SUMATHI RAJAMANI; GOBINATH KALYANA SUNDARAM; TAMIZHARASI SENGODAN; SIVAKUMAR THANGAVELU; NIKHITHA K SHANMUKHAN; ARUN RADHAKRISHNAN

doi:10.22159/ajpcr.2019.v12i2.29331

Authors

SUMATHI RAJAMANI Nandha College of Pharmacy and Research Institute, Erode - 52, Tamil Nadu, India.
GOBINATH KALYANA SUNDARAM Nandha College of Pharmacy and Research Institute, Erode - 52, Tamil Nadu, India.
TAMIZHARASI SENGODAN Nandha College of Pharmacy and Research Institute, Erode - 52, Tamil Nadu, India.
SIVAKUMAR THANGAVELU Nandha College of Pharmacy and Research Institute, Erode - 52, Tamil Nadu, India.
NIKHITHA K SHANMUKHAN Department of Pharmaceutics, JSS College of Pharmacy, Ootacamund, JSS Academy of Higher Education and Research, Mysuru, Karnataka, India.
ARUN RADHAKRISHNAN Department of Pharmaceutics, JSS College of Pharmacy, Ootacamund, JSS Academy of Higher Education and Research, Mysuru, Karnataka, India.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i2.29331

Keywords:

Carrageenan, Oxidative stress, Anti-inflammatory, Naringenin

Abstract

Objective: Naringenin (NAR) a flavonoid, exhibits extensive pharmacological action, fails to attain a significance in application due to low aqueous solubility (~ 0.214 mg/mL) which results in low bioavailability (5.8%). Nanosuspension of NAR (NARNS) was prepared in our previous studies using high-pressure homogenization employing various polymers. All these formulations were characterized and as a continuation of our work formulations was further evaluated for their anti-inflammatory activity by in vitro and in vivo methods.

Methods: Denaturation of protein method and membrane stabilization methods was chosen for in vitro evaluation. In vivo studies performed were acute inflammatory studies (carrageenan-induced paw edema) and chronic inflammatory studies (cotton pellet granuloma) on Wistar albino rats.

Results: The studies demonstrated that the NAR and NARNS at a dose of 50mg/kg P.O. have a potent activity compared to the standard drug diclofenac.

Conclusion: The percentage of protection against inflammation exhibited by NARNS was highly significant compared to NAR.

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Author Biography

ARUN RADHAKRISHNAN, Department of Pharmaceutics, JSS College of Pharmacy, Ootacamund, JSS Academy of Higher Education and Research, Mysuru, Karnataka, India.

Pharmaceutics

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