TRIGONELLA FOENUM-GRAECUM L. EXHIBITS ESTROGENIC EFFECT THROUGH PRESENILIN 2 GENE EXPRESSION IN THE BREAST CANCER CELL LINE MCF-7

KURNIA AGUSTINI; Michael Wink; Wahono Sumaryono; Frans Suyatna; Nurjati Chairani Siregar

doi:10.22159/ajpcr.2019.v12i1.29340

Authors

KURNIA AGUSTINI
Michael Wink Department of Biology, Institut fÃ¼r Pharmazie und Molekulare Biotechnologie (IPMB), Universitaet Heidelberg, Im Neuenheimer Feld 364, D-69120 Heidelberg, Germany.
Wahono Sumaryono Center for Pharmaceutical and Medical Technology, Agency for the Assessment and Application of Technology, LAPTIAB, PUSPIPTEK Area, Serpong, South Tangerang 15314, Indonesia.
Frans Suyatna Department of Pharmacology, Faculty of Medicine, University of Indonesia, Salemba Street No.6. Jakarta 10430, Indonesia.
Nurjati Chairani Siregar Department of Pharmacology, Faculty of Medicine, University of Indonesia, Salemba Street No.6. Jakarta 10430, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i1.29340

Keywords:

Estrogenic effect, MCF-7, Presenilin 2 gene, Trigonella-foenum graecum L

Abstract

Objective: The objective of this study is to investigate the estrogenic and antiestrogenic activity of Fenugreek seeds, Trigonella foenum-graecum L. in the estrogen-dependent breast cancer cell line, MCF-7, including its effect on the expression of estrogen-dependent presenilin 2 (pS2) gene.

Methods: An activity guided fractionation was carried out with extracts from fenugreek seeds in MCF-7 cells. Cytotoxic activity assays were conducted with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium (MTT) assay. Most fractions were also tested also tested in media with estradiol 10 nM We also analysed the expression of pS2 gene. For the analysis of pS2 gene expression we employed PCR primers for pS2 and for Î²-actin as a housekeeping gene using real-time polymerase chain reaction (RT-PCR).

Results: Based on cytotoxic activity assay in MCF-7, the active fractions are ethyl acetic fraction and its phases ethyl acetic (EA) 2 and EA 2.2. The most active fraction was EA 2.2 (IC50=27.129 ppm), which exhibited a biphasic effect; at low concentrations, it stimulated the growth, and at high concentrations it showed strong cytotoxic effects. EA2.2 fraction in concentration 20 ppm, also could induce pS2 gene expression in media with and without estrogen.

Conclusion: The most active fraction was the ethyl acetate phase and further subfractions. The most active fraction also induced the expression of pS2 gene which was studied by RT-PCR.

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