IN VITRO ANTIOXIDANT AND ANTI-INFLAMMATORY ACTIVITY OF THE FLOWER EXTRACTS OF OPUNTIA STRICTA

PRABHAKARAN D; RAJESHKANNA A; SENTHAMILSELVI MM

doi:10.22159/ajpcr.2019.v12i3.29930

Authors

PRABHAKARAN D Department of Chemistry, Periyar E.V.R. College (Autonomous), Trichy, Tamil Nadu, India.
RAJESHKANNA A Department of Chemistry, Periyar E.V.R. College (Autonomous), Trichy, Tamil Nadu, India.
SENTHAMILSELVI MM Department of Principal, Kamarajar Government Arts College, Surandai, Tamil Nadu, India.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i3.29930

Keywords:

Antioxidant, Peripheral blood mononuclear cells, Lipopolysaccharide, Opuntia stricta, Ferric-reducing capacity

Abstract

Objective: The objective of this study was to evaluate the antioxidant and anti-inflammatory activities of the solid powder obtained from the ethyl acetate fraction from the flower Opuntia stricta.

Methods: The flower extract was evaluated for antioxidant activity by 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay and reducing power assay was carried out by ferric-reducing capacity (FRC) assay method. The in vitro anti-inflammatory activity was evaluated using human peripheral blood mononuclear cells (PBMCs) stimulated by lipopolysaccharide (LPS) to evaluate nitric oxide (NO) production method.

Results: The solid powder obtained from the ethyl acetate fraction from the flower O. stricta showed a good antioxidant activity in scavenging DPPH radical and FRC assay with compared standard sample. This solid powder also showed good anti-inflammatory activity in cell viability (LPS-induced PBMCs) assay and NO assay.

Conclusion: These results suggest that the solid powder obtained from the ethyl acetate fraction from the flower O. stricta has significant antioxidant and anti-inflammatory activities.

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