ORAL ADMINISTRATION OF RAW WHITE KIDNEY BEANS (PHASEOLUS VULGARIS L. VAR. BELDIA) INDUCES OVERGROWTH OF FECAL COLIFORMS AND LACTOBACILLI IN WISTAR RATS

Nader Nciri; Taesub Shin; Namjun Cho

doi:10.22159/ajpcr.2018.v11s3.30036

Authors

Nader Nciri School of Energy, Materials and Chemical Engineering, Korea University of Technology and Education, Cheonan, Chungnam, Republic of Korea.
Taesub Shin School of Energy, Materials and Chemical Engineering, Korea University of Technology and Education, Cheonan, Chungnam, Republic of Korea.
Namjun Cho School of Energy, Materials and Chemical Engineering, Korea University of Technology and Education, Cheonan, Chungnam, Republic of Korea.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11s3.30036

Keywords:

Body weight, Coliforms, Fecal microflora, Food intake, Internal organs, Lactobacilli, Phytohemagglutinin, White kidney bean (Phaseolus vulgaris L)

Abstract

Objective: The comprehensive dynamics of fecal microbiota in response to the ingestion of toxic bean lectins or phytohemagglutinins has not been well studied. The study aimed at evaluating the gavage effects of a raw Beldia bean variety on food intake, growth performance, gastrointestinal organs, and fecal microflora in Wistar rats.

Methods: Twenty young adult male rats were randomly allotted into two groups of 10 rats each: Control rats were gavaged with 300 mg of a rodent pellet flour suspension and experimental rats were orogastrically fed a dose of 300 mg Beldia bean flour suspension (BBFS). Individual food intake, body weight, and fecal score were taken daily. To assess the impact on the gut flora, fecal samples were collected every day for 10 days. All animals were sacrificed on day 10, to obtain blood and internal organs samples.

Results: The results revealed that the gavage of a BBFS to rats had no marked influence on average daily of food intake and weight gain. No significant differences were found in the weights of the small intestine, spleen, liver, and thymus of rats given raw Beldia diet. The counts of coliforms and lactobacilli on pooled fecal specimens of BBFS-fed rats were increased significantly compared to controls.

Conclusion: In summary, the exposure to raw Beldia beans altered the fecal microbiota, without adverse effects on animals.

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References

Pusztai A, Palmer R. Nutritional evaluation of kidney beans (Phaseolus vulgaris): The toxic principle. J Sci Food Agric 1977;28:620-23.

Banwell JG, Boldt DH, Meyers J, Weber FL Jr. Phytohemagglutinin derived from red kidney bean (Phaseolus vulgaris): A cause for intestinal malabsorption associated with bacterial overgrowth in the rat. Gastroenterology 1983;84:506-15.

Banwell JG, Abramowsky CR, Weber F, Howard R, Boldt DH. Phytohemagglutinin-induced diarrheal disease. Dig Dis Sci 1984;29:921-9.

King TP, Pusztai A, Clarke EMW. Kidney bean (Phaseolus vulgaris) lectin-induced lesions in rat small intestine: 1. Light microscope studies. J Comp Pathol 1980;90:585-95.

Wilson AB, King TP, Clarke EM, Pusztai A. Kidney bean (Phaseolus vulgaris) lectin-induced lesions in rat small intestine: 2. Microbiological studies. J Comp Pathol 1980;90:597-602.

Banwell JG, Howard R, Cooper D, Costerton JW. Intestinal microbial flora after feeding phytohemagglutinin lectins (Phaseolus vulgaris) to rats. Appl Environ Microbiol 1985;50:68-80.

Banwell JG, Howard R, Kabir I, Costerton JW. Bacterial overgrowth by indigenous microflora in the phytohemagglutinin-fed rat. Can J Microbiol 1988;34:1009-13.

Helrich K, editor. AOAC: Official Methods of Analytical Chemists. Vol.1. Arlington, Virginia, USA: Association of Official Analytical Chemists, Inc.; 1990.

Weinman MD, Allan CH, Trier JS, Hagen SJ. Repair of microvilli in the rat small intestine after damage with lectins contained in the red kidney bean. Gastroenterology 1989;97:1193-204.

Hagen SJ, Trier JS, Dambrauskas R. Exposure of the rat small intestine to raw kidney beans results in reorganization of absorptive cell microvilli. Gastroenterology 1994;106:73-84.

Gross G, Wildner J, Schonewille A, Rademaker JL, van der Meer R, Snel J. Probiotic Lactobacillus plantarum 299v does not counteract unfavorable phytohemagglutinin-induced changes in the rat intestinal microbiota. Appl Environ Microbiol 2008;74:5244-49.

Ellinger DK, Muller LD, Glantz PJ. Influence of feeding fermented colostrum and Lactobacillus acidophilus on fecal flora of dairy claves. J Dairy Sci 1980;63:478-82.

Fantini N, Cabras C, Lobina C, Colombo G, Gessa GL, Riva A, et al. Reducing effect of a Phaseolus vulgaris dry extract on food intake, body weight, and glycemia in rats. J Agric Food Chem 2009;57:9316 23.

Marzo F, Alonso R, Urdaneta E, Arricibita FJ, IbÃ¡Ã±ez F. Nutritional quality of extruded kidney bean (Phaseolus vulgaris L. Var. Pinto) and its effects on growth and skeletal muscle nitrogen fractions in rats. J Anim Sci 2002;80:875-9.

Lafont J, Rouanet JM, Gabrion J, Assouad JL, Zambonino Infante JL, BesanÃ§on P, et al. Duodenal toxicity of dietary phaseolus vulgaris lectins in the rat: An integrative assay. Digestion 1988;41:83-93.

Baintner K, Kiss P, PfÃ¼ller U, Bardocz S, Pusztai A. Effect of orally and intraperitoneally administered plant lectins on food consumption of rats. Acta Physiol Hung 2003;90:97-107.

Rouanet JM, Lafont J, Chalet M, Creppy A, Besancon P. Effects of dietary kidney bean (Phaseolus vulgaris) lectins in growing rats. Nutr Rep Int 1985;31:237-44.

Cheeke PR. Toxicants of plant origin. Vol. 3. In: Proteins and Amino Acids. Boca Raton, Florida: CRC Press, Inc.; 1989.

Pereira LL, Pereira CA, de Sousa RV, dos Santos CD, de Moraes CF, SÃ¡tiro LC. White bean flour (Phaseolus vulgaris): Therapeutic and toxicological research in wistar rats. J Appl Pharm Sci 2002;2:1-7.

JaffÃ© WG, Lette CL. Heat-labile growth-inhibiting factors in beans (Phaseolus vulgaris). J Nutr 1968;94:203-10.

Herzig KH, Bardocz S, Grant G, Nustede R, FÃ¶lsch UR, Pusztai A, et al. Red kidney bean lectin is a potent cholecystokinin releasing stimulus in the rat inducing pancreatic growth. Gut 1997;41:333-8.

RÃ¥dberg K, Biernat M, Linderoth A, Zabielski R, Pierzynowski SG, WestrÃ¶m BR. Enteral exposure to crude red kidney bean lectin induces maturation of the gut in suckling pigs. J Anim Sci 2001;79:2669-78.

JaffÃ© WG, GÃ³mez MJ. Beans of high or low toxicity. Plant Food Hum Nutr 1975;24:359-65.

Pusztai A, Clarke EM, King TP, Stewart JC. Nutritional evaluation of kidney beans (Phaseolus vulgaris): chemical composition, lectin content and nutritional value of selected cultivars. J Sci Food Agric 1979;30:843-8.

JaffÃ© WG, BrÃ¼cher O, Palozzo A. Detection of four types of specific phytohemagglutinins in different lines of beans (Phaseolus vulgaris). Z Immunitatsforsch Exp Klin Immunol 1972;142:439-47.

JaffÃ© WG. Factors affecting the nutritional value of beans. In: Milner M, editor. Nutritional Improvement of Beans. New York: Protein Advisory group of U.N. System; 1973.

Grant G, More LJ, McKenzie NH, Stewart JC, Pusztai A. A survey of the nutritional and haemagglutination properties of legume seeds generally available in the UK. Br J Nutr 1983;50:207-14.

Pusztai A, Grant G, Stewart JC. A new type of Phaseolus vulgaris (cv. Pinto III) seed lectin: Isolation and characterization. Biochim Biophys Acta 1981;671:146-54.

Pusztai A. Plant Lectins. Cambridge, UK: Cambridge University Press; 1991.

Cuatrecasas P, Tell GP. Insulin-like activity of concanavalin A and wheat germ agglutinin â€“ direct interactions with insulin receptors. Proc Natl Acad Sci U S A 1973;70:485-9.

Grant G, de Oliveira JT, Dorward PM, Annand MG, Waldron M, Pusztai A. Metabolic and hormonal changes in rats resulting from consumption of kidney bean (Phaseolus vulgaris) or soybean (Glycine max). Nutr Rep Int 1987;36:763-72.

Pusztai A, Greer F, Lima MD, Prouvost-Danon A, King TP. Local and systemic responses to dietary lectins. In: Goldstein IJ, Etzler ME, editors. Chemical Taxonomy, Molecular Biology and Function of Plant Lectins. New York, NY: Alan R. Liss, Inc.; 1983. p. 271-72.

Oliveira JT, Vasconcelos IM, Gondim MJ, Cavada BS, Moreira RA, Santos CF, et al. Canavalia brasiliensis seeds. Protein quality and nutritional implications of dietary lectin. J Sci Food Agric 1994;64:417 24.

Greer F, Brewer AC, Pusztai A. Effect of kidney bean (Phaseolus vulgaris) toxin on tissue weight and composition and some metabolic functions of rats. Br J Nutr 1985;54:95-103.

Delgado E, Vences-MontaÃ±o MI, RodrÃguez JV, Rocha-Guzman N, Rodriguez-Vidal A, Herrera-Gonzalez SM, et al. Inhibition of the growth of rats by extruded snacks from bean (Phaseolus vulgaris) and corn (Zea mays). Emir J Food Agric 2012;24:255-63.

De Oliveira JT, Pusztai A, Grant G. Changes in organs and tissues induced by feeding of purified kidney bean (Phaseolus vulgaris) lectins. Nutr Res 1988;8:943-47.

Linderoth A, Prykhodâ€™ko O, Pierzynowski SG, WestrÃ¶m BR. Enterally but not parenterally administered Phaseolus vulgaris lectin induces growth and precocious maturation of the gut in suckling rats. Biol Neonate 2006;89:60-8.

Filip R, Wdowiak L, Harrison AP, Pierzynowski SG. Dietary supplementation with phytohemagglutinin in combination with Î±-ketoglutarate limits the excretion of nitrogen via urinary tract. Ann Agric Environ Med 2008;15:309-15.

Reynoso-Camacho R, GonzÃ¡lez de MejÃa E, Loarca-PiÃ±a G. Purification and acute toxicity of a lectin extracted from tepary bean (Phaseolus acutifolius). Food Chem Toxicol 2003;41:21-7.

Nciri N, Cho N, Bergaoui N, El Mhamdi F, Ben Ammar A, Trabelsi N, et al. Effect of white kidney beans (Phaseolus vulgaris L. Var. Beldia) on small intestine morphology and function in wistar rats. J Med Food 2015;18:1387-99.

Nciri N, Cho N, El Mhamdi F, Ben Ismail H, Ben Mansour A, Sassi FH, et al. Toxicity assessment of common beans (Phaseolus vulgaris L.) widely consumed by tunisian population. J Med Food 2015;18:1049 64.