CULTURED HAIR FOLLICLE CELLS FOR THE TREATMENT OF VITILIGO

Authors

  • Piyatida Sutnut Department of Biological Engineering Program, Faculty of Engineering, KMUTT, Bangkok, Thailand.
  • Saroj Suvanasuthi Department of Samitivej Sukhumvit Hospital, 33 Sukhumvit 49, Klongtan Nua, Vaghana, Bangkok, Thailand.
  • Kwanchanok Viravaidya-pasuwat Department of Biological Engineering Program, Faculty of Engineering, KMUTT, Bangkok, Thailand.

DOI:

https://doi.org/10.22159/ajpcr.2018.v11s3.30043

Keywords:

Hair follicles, Hydrogel, Nil, Vitiligo

Abstract

Objective: The objective of this study was to develop a method to isolate cells from hair follicles and cultured them in a hydrogel.

Methods: Different cell types obtained from hair follicles were investigated and mixed with three formulations of Lutrol® F-127-based hydrogels. The percentages of the cell attachment and viability were observed within 48 h.

Results: The results showed that three cell types, including keratinocyte, dermal papilla, and melanocyte cells, were obtained, as shown by the expression of their corresponding genes. All formulations of the hydrogels supported cell attachment and viability. Interestingly, more than 60% cell attachment and viability were found in lutrol hydrogels supplemented with either fetal bovine serum (FBS) or heat-activated human serum.

Conclusion: Higher cell attachment and viability were observed when the hair follicle cells were cultured in the hydrogel with FBS than the hydrogel with human serum. However, the lutrol gel formulation with human serum was more appropriate to be used in the future clinical study, as this formulation contained no animal-derived component.

Downloads

Download data is not yet available.

References

Alzolibani AA, Al Robaee A, Zedan K. Genetic epidemiology and heritability of vitiligo. Acta Dermatovenerol Alp Pannonica Adriat 2011;3:74.

Lakhani DM, Deshpande AS. Various treatments for vitiligo: Problems associated and solutions. J Appl Pharm Sci 2014;4:101-5.

Majid I. Does topical tacrolimus ointment enhance the efficacy of narrowband ultraviolet B therapy in vitiligo? A left-right comparison study. Photodermatol Photoimmunol Photomed 2010;26:230-4.

Singh C, Parsad D, Kanwar AJ, Dogra S, Kumar R. Comparison between autologous noncultured extracted hair follicle outer root sheath cell suspension and autologous noncultured epidermal cell suspension in the treatment of stable vitiligo: A randomized study. Br J Dermatol 2013;169:287-93.

Hirobe T. Keratinocytes regulate the function of melanocytes. Dermatol Sinica 2014;32:200-4.

Diniz IM, Chen C, Xu X, Ansari S, Zadeh HH, Marques MM, et al. Pluronic F-127 hydrogel as a promising scaffold for encapsulation of dental-derived mesenchymal stem cells. J Mater Sci Mater Med 2015;26:153.

Na GY, Paek SH, Park BC, Kim DW, Lee WJ, Lee SJ, et al. Isolation and characterization of outer root sheath melanocytes of human hair follicles. Br J Dermatol 2006;155:902-9.

Sorrell JM, Caplan AI. Fibroblast heterogeneity: More than skin deep. J Cell Sci 2004;117:667-75.

Borowiec AS, Delcourt P, Dewailly E, Bidaux G. Optimal differentiation of in vitro keratinocytes requires multifactorial external control. PLoS One 2013;8:e77507.

Vetrini F, Auricchio A, Du J, Angeletti B, Fisher DE, Ballabio A, et al. The microphthalmia transcription factor (Mitf) controls expression of the ocular albinism Type 1 gene: Link between melanin synthesis and melanosome biogenesis. Mol Cell Biol 2004;24:6550-9.

Published

06-10-2018

How to Cite

Sutnut, P., S. Suvanasuthi, and K. Viravaidya-pasuwat. “CULTURED HAIR FOLLICLE CELLS FOR THE TREATMENT OF VITILIGO”. Asian Journal of Pharmaceutical and Clinical Research, vol. 11, no. 15, Oct. 2018, pp. 94-97, doi:10.22159/ajpcr.2018.v11s3.30043.

Issue

Section

Original Article(s)