NARINGIN ATTENUATES OXIDATIVE STRESS AND PROTECT AGAINST MYOCARDIAL ISCHEMIA-REPERFUSION INJURY IN DIABETIC RAT

NEELAM KUMARI; AJAY SINGH KUSHWAH

doi:10.22159/ajpcr.2019.v12i3.30105

Authors

NEELAM KUMARI Department of Pharmacology, Amar Shaheed Baba Ajit Singh Jujhar Singh Memorial College of Pharmacy, Ropar, Punjab, India.
AJAY SINGH KUSHWAH Department of Pharmacology, Amar Shaheed Baba Ajit Singh Jujhar Singh Memorial College of Pharmacy, Ropar, Punjab, India. http://orcid.org/0000-0003-0559-7670

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i3.30105

Keywords:

Diabetes, Ischemia-reperfusion injury, Naringin

Abstract

Objective: The relative risk of coronary heart disease in diabetic patients is more than in non-diabetic population. The present study was undertaken to explore the cardioprotective effect of Naringin on ischemia-reperfusion injury in the diabetic model of rat.

Methods: Adult Wistar rats (either sex) divided into six groups. Diabetes was induced by 5 weeks combine exposure to a high-fat diet with a low dose of streptozotocin (30 mg/kg i.p.), administered on the 1st day of starting of the 5th week. Naringin treatment 25 mg/kg and 50 mg/kg was given simultaneously for 5 weeks. On the 36th day, the study animals were subjected to induction myocardial ischemia-reperfusion injury induced by the ligation of the left anterior descending coronary artery ligation in anesthetizing rat. Serum glucose level and cholesterol level measured before performing of ischemic reperfusion. After reperfusion injury, the animals were sacrificed and estimate change in the heart in the course of biochemical alterations, in creatine kinase-muscle/ brain (CK-MB) and lactate dehydrogenase, lipid peroxidation (LPO), glutathione (GSH), superoxide dismutase (SOD) and infarct size in the heart.

Results and Conclusion: Naringin treatment significantly reduced the body weight, blood glucose, cholesterol, cardiac injury biomarkers, and LPO level and increased in antioxidant (GSH and SOD) level and also significantly increased in mean arterial pressure heart rate, reduced the myocardial infarction size. The present study concludes that Naringin 50 mg/kg being more prominent action to reduce the cardiotoxicity risk in ischemia-reperfusion injury state and increases myocardial susceptibility through having more prominent antioxidant potential properties.

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Author Biographies

NEELAM KUMARI, Department of Pharmacology, Amar Shaheed Baba Ajit Singh Jujhar Singh Memorial College of Pharmacy, Ropar, Punjab, India.

M.Pharm Research Scholer, Department of Pharmacology
ASBASJS Memorial College of Pharmacy, Bela-140111, Ropar, (Punjab) India
Affiliated I.K. Gujral Punjab Technical University, Jalandhar- 144603 (Punjab) India

AJAY SINGH KUSHWAH, Department of Pharmacology, Amar Shaheed Baba Ajit Singh Jujhar Singh Memorial College of Pharmacy, Ropar, Punjab, India.

Ajay Singh Kushwah Assistant Professor Department of Pharmacology ASBASJS Memorial College of Pharmacy, Bela-140111, Ropar, (Punjab) India, Tele: +91-9417459195, FAX-01881-263655, E-mail: kushwah_ph05@yahoo.co.in

References

Al-Nozha MM, Ismail HM, Al Nozha OM. Coronary artery disease and diabetes mellitus. J Taibah Univ Med Sci 2016;11:330-8.

Paulson DJ. The diabetic heart is more sensitive to ischemic injury. Cardiovasc Res 1997;34:104-12.

Patel AC, Pathak LN, Bhatt N, Marya B, Gavania M, Trivedi H. Review: Cardio vascular cmplication of diabetes mellitus. J Appl Pharm Sci 2011;1:1-6.

Forbes JM, Cooper ME. Mechanism of diabetic complication. Physiol Rev 2013;93:137-88.

Lejay A, Fang F, John R, Van JA, Barr M, Thaveau F, et al. Ischemia reperfusion injury, ischemia conditioning and diabetes mellitus. J Mol Cell Cardiol 2016;91:11-22.

Giacco F, Brownlee M. Oxidative stress and diabetic complications. Circ Res 2010;107:1058-70.

Hoffman JW Jr., Gilbert TB, Poston RS, Silldorff EP. Myocardial reperfusion injury: Etiology, mechanisms, and therapies. J Extra Corpor Technol 2004;36:391-411.

Bharti S, Rani N, Krishnamurthy B, Arya DS. Preclinical evidence for the pharmacological actions of naringin: A review. Planta Med 2014;80:437-51.

Alam MA, Kauter K, Brown L. Naringin improves diet-induced cardiovascular dysfunction and obesity in high carbohydrate, high fat diet-fed rats. Nutrients 2013;5:637-50.

Cao X, Lin W, Liang C, Zhang D, Yang F, Zhang Y, et al. Naringin rescued the TNF-α-induced inhibition of osteogenesis of bone marrow-derived mesenchymal stem cells by depressing the activation of NF-кB signaling pathway. Immunol Res 2015;62:357-67.

Liu Y, Wu H, Nie YC, Chen JL, Su WW, Li PB, et al. Naringin attenuates acute lung injury in LPS-treated mice by inhibiting NF-κB pathway. Int Immunopharmacol 2011;11:1606-12.

Singh D, Chopra K. The effect of naringin, a bioflavonoid on ischemia-reperfusion induced renal injury in rats. Pharmacol Res 2004;50:187-93.

Fu F, Tian F, Zhou H, Lv W, Tie R, Ji L, et al. Semen cassiae attenuates myocardial ischemia and reperfusion injury in high-fat diet streptozotocin-induced Type 2 diabetic rats. Am J Chin Med 2014;42:95-108.

Ito WD, Schaarschmidt S, Klask R, Hansen S, Schäfer HJ, Mathey D, et al. Infarct size measurement by triphenyltetrazolium chloride staining versus in vivo injection of propidium iodide. J Mol Cell Cardiol 1997;29:2169-75.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95:351-8.

Ellman GL. Tissue sulfhydryl groups. Arch Biochem Biophys 1959;82:70-7.

Kono Y. Generation of superoxide radical during autoxidation of hydroxylamine and an assay for superoxide dismutase. Arch Biochem Biophys 1978;186:189-95.

Kahn SE, Hull RL, Utzschneider KM. Mechanisms linking obesity to insulin resistance and Type 2 diabetes. Nature 2006;444:840-6.

Pu P, Gao DM, Mohamed S, Chen J, Zhang J, Zhou XY, et al. Naringin ameliorates metabolic syndrome by activating AMP-activated protein kinase in mice fed a high-fat diet. Arch Biochem Biophys 2012;518:61-70.

Galiñanes M, Fowler AG. Role of clinical pathologies in myocardial injury following ischaemia and reperfusion. Cardiovasc Res 2004;61:512-21.

Ramezani-Aliakbari F, Badavi M, Dianat M, Mard SA, Ahangarpour A. Effects of gallic acid on hemodynamic parameters and infarct size after ischemia-reperfusion in isolated rat hearts with alloxan-induced diabetes. Biomed Pharmacother 2017;96:612-8.

Abdelhalim AT, Nur NM, Mansour S, Ibrahim A. Cardioprotective effect of Vitamin E against myocardial infarction induced by isoprenaline in albino rats. Asian J Pharm Clin Res 2018;11:273-6.

Pieme CA, Tatangmo JA, Simo G, Biapa Nya PC, Ama Moor VJ, Moukette Moukette B, et al. Relationship between hyperglycemia, antioxidant capacity and some enzymatic and non-enzymatic antioxidants in african patients with Type 2 diabetes. BMC Res Notes 2017;10:141.

Hegde K, Patel D, Varma K. Evaluation of cardioprotective activity of aqueous extract of Garcinia indica Linn fruit rind. Asian J Pharm Clin Res 2015;8:107-12.

You Q, Wu Z, Wu B, Liu C, Huang R, Yang L, et al. Naringin protects cardiomyocytes against hyperglycemia-induced injuries in vitro and in vivo. J Endocrinol 2016;230:197-214.

Ansley DM, Wang B. Oxidative stress and myocardial injury in the diabetic heart. J Pathol 2013;229:232-41.

Pan GZ, Xie J, Tian XF, Yang SW, Zhou YJ. The impact of different plasma glucose levels on heart rate in experimental rats with acute myocardial infarction. Cardiol Res 2016;7:146-51.