THE CYTOPROTECTIVE ACTIVITY OF AQUEOUS GREEN TEA EXTRACT AGAINST METRONIDAZOLE AND TINIDAZOLE GENOTOXIC EFFECT

Ali Faris Hassan; Mahmood Kahtan; Nada Naje Al-shawi; Intesar Tareq Numan; Ghaith Abbas Bahader

doi:10.22159/ajpcr.2019.v12i1.30363

Authors

Ali Faris Hassan Department of Pharmacology and Toxicology, College of Pharmacy, University of Baghdad, Baghdad, Iraq.
Mahmood Kahtan Department of Pharmacology and Toxicology, College of Pharmacy, University of Baghdad, Baghdad, Iraq.
Nada Naje Al-shawi Department of Pharmacology and Toxicology, College of Pharmacy, University of Baghdad, Baghdad, Iraq.
Intesar Tareq Numan Department of Pharmacology and Toxicology, College of Pharmacy, University of AL-Bayan, Baghdad, Iraq.
Ghaith Abbas Bahader Department of Pharmaceutical Chemistry, College of Pharmacy, University of Baghdad, Baghdad, Iraq.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i1.30363

Keywords:

Green tea, Metronidazole, Micronucleus, Mitotic index, Tinidazole

Abstract

Objective: The study was designed to evaluate the genoprotective effect of green tea extract against genotoxicity induced by metronidazole and tinidazole.

Methods: A total of 36 mice were used, for each experiment. The animals were allocated into six groups: Group I - negative control administered distilled water; Group II - healthy mice treated with metronidazole alone, Group III - healthy mice treated with tinidazole alone, Group IV - healthy mice administered green tea extract alone, Group V - healthy mice administered of metronidazole anddd then green tea extract was administered, and Group VI - healthy mice administered of tinidazole and then green tea extract was administered.

Results: It has been found that there are significant differences in mitotic index and micronucleus (MN) appearance between the Groups II, IV, and V (7.76Â±0.8, 11.92Â±1.14, and 8.36Â±0.57 and 6.92Â±0.5, 10.18Â±1.19, and 7.52Â±1.05) in bone marrow cells and spleen cells for mitotic index, respectively (6.75Â±0.4, 5.5Â±0.41, and 5.92Â±0.68), in bone marrow for MN appearance. Furthermore, it has been found that there are significant differences in mitotic index and MN appearance between the Groups III, IV, and VI (4.36Â±0.88, 11.92Â±1.14, and 5.36Â±0.55 and 4.08Â±0.35, 10.18Â±1.19, and 5.08Â±0.35) in bone marrow cells and spleen cells for mitotic index, respectively (8.43Â±0.96, 5.5Â±0.41, and 6.84Â±0.66), in bone marrow for MN appearance.

Conclusion: The aqueous extract of green tea has a protective effect against the genotoxic effects induced by either metronidazole or tinidazole in mice.

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Author Biography

Ali Faris Hassan, Department of Pharmacology and Toxicology, College of Pharmacy, University of Baghdad, Baghdad, Iraq.

lecturer at pharmacology and toxicology depaetment . had PhD degree at pharmacology and toxicology -genotoxicity

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