EVALUATION OF GROWTH INHIBITORY POTENTIAL OF MORINGA OLEIFERA FLOWERS ON PC3 CELL LINES
Abstract
Objective: PC3 cell lines are the classical in vitro androgen-independent models of prostate cancer with high metastatic potential. Moringa oleifera is a predominant Indian nutritional plant with high medicinal value. The objective of the study was to investigate the flowers of this traditional plant for their potential to inhibit the growth of cancer cells in PC3 cell lines. Methods: The growth response of cancer cells to the methanol extract of M. oleifera flowers was analyzed by MTT (3-4,5-dimethylthiazol-2-yl 2,5-diphenyl tetrazolium bromide) assay and compared to that of known common standard therapeutics. Similarly, the plant material was also tested for its effect on normal cells. Results: While Turmeric and Curcumin showed cancer cell growth inhibition at higher concentrations, M. oleifera flower extract exhibited a gradual dose-dependent decrease in the percentage of cancer cell growth from 0.01µg/ml onwards with its concentration being 46.91 µg/ml for fifty percent growth inhibition (GI50). Also, while the plant extract did not affect the cell viability of normal cells, the other two showed insignificant changes. Conclusion: The results suggested the presence of potent anticancer compounds in the M. oleifera flower extract, non-toxic to normal cells, but responsible for its effective growth inhibition of PC3 cells. This further signified the application of M. oleifera flowers as the efficacious source of natural therapeutics against androgen-independent prostate cancer.
Keywords: PC3 cell lines, androgen-independent prostate cancer, Moringa oleifera, MTT assay, GI50
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Shiow YW, Kim SL. Antioxidant Activities and Anticancer Cell Proliferation Properties of Wild Strawberries. J Amer Soc Hort Sci 2007; 132(5): 647-658.
Harwell JL. Plants used against cancer: a survey. Lloydia 1971; 34: 204-255.
Inbathamizh L, Padmini E. Effect of geographical properties on the phytochemical composition and antioxidant potential of Moringa oleifera flowers. BioMedRx 2013; 1(3): 239-247.
Nachshon Kedmi M, Yannai S, Fares FA. Induction of apoptosis in human prostate cancer cell line, PC3, by 3, 3′-diindolylmethane through the mitochondrial pathway. Br J Cancer 2004; 91(7): 1358–1363.
Younghun Jung, Yusuke Shiozawa, Jingcheng Wang, Natalie McGregor, Jinlu Dai, Serk In Park, et al. Prevalence of Prostate Cancer Metastases after Intravenous Inoculation Provides Clues into the Molecular Basis of Dormancy in the Bone Marrow Microenvironment. Neoplasia 2012; 14(5): 429–439.
Monali P Yeole, Shashikanth N Dhole, Nilesh S Kulkarni. Peptide nanomedicine in cancer treatment. Asian J Pharm Clin Res 2013; 6(Suppl 2): 28-32.
Aggarwal BB, Sundaram C, Malani N, Ichikawa H. Curcumin: the Indian solid gold. Adv Exp Med Biol 2007; 595: 1-75.
Harish K Handral, Shrishail Duggi, Ravichandra Handral, Tulsianand G, Shruthi SD. Turmeric: Nature’s precious medicine. Asian J Pharm Clin Res 2013; 6(3): 10-16.
Sharma RA, McLelland HR, Hill KA, Ireson CR, Euden SA, Manson MM, et al. Pharmacodynamic and pharmacokinetic study of oral Curcuma extract in patients with colorectal cancer. Clin Cancer Res 2001; 7(7): 1894-1900.
Aggarwal S, Ichikawa H, Takada Y, Sandur SK, Shishodia S, Aggarwal BB. Curcumin (diferuloylmethane) down-regulates expression of cell proliferation and antiapoptotic and metastatic gene products through suppression of IkappaBalpha kinase and Akt activation. Mol Pharmacol 2006; 69(1): 195-206.
Eloff JN. A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Med 1998; 64(8): 711-713.
Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of radiosensitivity. Cancer Res 1987; 47(4): 943-946.
Romijn JC, Verkoelen CF, Schroeder FH. Application of the MTT assay to human prostate cancer cell lines in vitro: Establishment of test conditions and assessment of hormone-stimulated growth and drug-induced cytostatic and cytotoxic effects. Prostate 1988; 12(1): 99–110.
Ding M, Lu Y, Bowman L, Huang C, Leonard S, Wang L, et al. Inhibition of AP-1 and neoplastic transformation by fresh apple peel extract. J Biol Chem 2004; 279(11): 10670-10676.
Wang SY, Feng R, Lu Y, Bowman L, Ding M. Inhibitory effect on activator protein-1, nuclear factor-kappaB, and cell transformation by extracts of strawberries (Fragaria X ananassa Duch.). J Agric Food Chem 2005; 53(10): 4187-4193.
Olsson ME, Andersson CS, Oredsson SM, Berglund RH, Gustavsson KE. Antioxidant levels and inhibition of cancer cell proliferation in vitro by extracts from organically and conventionally cultivated strawberries. J Agr Food Chem 2006; 54: 1248-1255.
Kirana C, Record I, McIntosh G, Jones G. Screening for antitumor activity of 11 Species of Indonesian Zingiberaceae using human MCF-7 and HT-29 cancer cells. Pharm Biol 2003; 41(4): 271–276.
Agarwal C, Sharma Y, Agarwal R. Anticarcinogenic effect of a polyphenolic fraction isolated from grape seeds in human prostate carcinoma DU145 cells: modulation of mitogenic signaling and cell-cycle regulators and induction of G1 arrest and apoptosis. Mol Carcinog 2000; 28(3): 129-138.
Izevbigie EB, Bryant JL, Walker A. A novel natural inhibitor of extracellular signal-regulated kinases and human breast cancer cell growth. Exp Biol Med 2004; 229(2): 163-169.
Zhang CL, Wu LJ, Tashiro S, Onodera S, Ikejima T. Oridonin induces caspase-independent but mitochondria- and MAPK-dependent cell death in the murine fibrosarcoma cell line L929. Biol Pharm Bull 2004; 27(10): 1527-1531.
Costa-Lotufo LV, Khan MTH, Ather A, Wilke DV, Jimenez PC, Pessoa C. Studies of the anticancer potential of plants used in Bangladeshi folk medicine. J Ethnopharmacol 2005; 99(1): 21–30.
Guevara AP, Vargas C, Sakurai H, Fujiwara Y, Hashimoto K, Maoka T, et al. An antitumor promoter from Moringa oleifera Lam. Mutat Res 1999; 440: 181-188.
Murakami A, Kitazono Y, Jiwajinda S, Koshimizu K, Ohigashi H. Niaziminin, a thiocarbamate from the leaves of Moringa oleifera, holds a strict structural requirement for inhibition of tumor-promoter- induced Epstein-Barr virus activation. Planta Med 1998; 64: 319-323.
Consolacion Y Ragasa, Ruel M Levida, Ming-Jaw Don, Chien-Chang Shen. Cytotoxic Isothiocyanates from Moringa oleifera Lam Seeds. Philippine Science Letters 2012; 5(1): 46-52.
Bharali R, Tabassum J, Azad MR. Chemomodulatory effect of Moringa oleifera, Lam, on hepatic carcinogen metabolizing enzymes, antioxidant parameters and skin papillomagenesis in mice. Asian Pac J Cancer Prev 2003; 4(2): 131-139.
Parvathy MV, Umaheshswari A. Cytotoxic effect of Moringa oleifera leaf extracts on human multiple myeloma cell lines. Trends Med Res 2007; 1: 44-50.
Shruti Nair, Varalakshmi KN. Anticancer, cytotoxic potential of Moringa oleifera extracts on HeLa cell line. J Nat Pharm 2011; 2(3): 138-142.
Hermawan A, Nur KS, Dewi D, Putri P, Meiyanto E. Ethanolic Extract of Moringa oleifera Increased Cytotoxic Effect of Doxorubicin on Hela Cancer Cells. J Nat Remedies 2012; 12(2): 108-114.
Leszczyniecka M, Roberts T, Dent P, Grant S, Fisher PB. Differentiation therapy of human cancer: basic science and clinical applications. Pharmacol Ther 2001; 90(2-3): 105-156.
James SY, Williams MA, Newland AC, Colston KW. Leukemia cell differentiation: cellular and molecular interactions of retinoids and vitamin D. Gen Pharmacol 1999; 32: 143–154.
Han ZT, Zhu XX, Yang RY, Sun JZ, Tian GF, Liu XJ, et al. Effect of intravenous infusions of 12-O-tetradecanoylphorbol-13-acetate (TPA) in patients with myelocytic leukemia: preliminary studies on therapeutic efficacy and toxicity. Proc Natl Acad Sci 1998; 95(9): 5357-5361.
Tilley WD, Wilson CM, Marcelli M, McPhaul MJ. Androgen receptor gene expression in human prostate carcinoma cell lines. Cancer Res 1990; 50(17): 5382-5386.
Daniel Nowak, Daphne Stewart, Phillip Koeffler H. Differentiation therapy of leukemia: 3 decades of development. Blood 2009; 113(16): 3655-3665.
Samid D, Shack S, Myers CE. Selective growth arrest and phenotypic reversion of prostate cancer cells in vitro by nontoxic pharmacological concentrations of phenylacetate. J Clin Invest 1993; 91(5): 2288–2295.
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