CARDIOPROTECTIVE EFFECT OF FISETIN ON LYSOSOMAL ENZYME ACTIVITIES OF NORMAL AND ISOPROTERENOL-INDUCED MYOCARDIAL INFARCTION IN MALE WISTAR RATS

MYTHILY  PANNEERSELVAM; DEVIKA P. T

doi:10.22159/ajpcr.2019.v12i3.30974

Authors

MYTHILY PANNEERSELVAM Department of Biochemistry, Mohamed Sathak College of Arts and Science, Chennai, Tamil Nadu, India.
DEVIKA P. T Department of Biochemistry, Mohamed Sathak College of Arts and Science, Chennai, Tamil Nadu, India.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i3.30974

Keywords:

Myocardial infarction, Isoproterenol, Fisetin and Lysosomal enzymes

Abstract

Objective: This study aims to evaluate the cardioprotective effect of fisetin on change in the activities of lysosomal enzymes in isoproterenol (ISO)- induced myocardial infarction (MI) in male albino Wistar rats.

Methods: Rats were orally pretreated with fisetin (30 mg/kg body weight) daily for 30 days. After the pretreatment period, ISO (100 mg/kg body weight) was subcutaneously administered to rats at intervals of 24 h for 2 consecutive days.

Results: The activities of β-glucuronidase, β-N-acetylglucosaminidase, β-galactosidase, cathepsin-B, and cathepsin-D were significantly (*p<0.05) increased in serum and the heart of ISO-induced rats. Pre-treatment with fisetin daily for 30 days to ISO-induced rats significantly prevented these activities and restored their activities to near normal. Oral treatment with fisetin (30 mg/kg body weight) to normal control rats did not show any significant effect.

Conclusion: We have concluded that fisetin protects the β-glucuronidase, β-N-acetylglucosaminidase, β-galactosidase, cathepsin-B, and cathepsin-D against ISO-induced MI. The observed effects might be due to the free radical-scavenging and membrane-stabilizing effect of fisetin.

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References

Aronow WS. Epidemiology, pathophysiology, prognosis, and treatment of systolic and diastolic heart failure. Cardiol Rev 2006;14:108-24.

Boudina S, Laclau MN, Tariosse L, Daret D, Gouverneur G, Bonoron- Adèle S, et al. Alteration of mitochondrial function in a model of chronic ischemia in vivo in rat heart. Am J Physiol Heart Circ Physiol 2002;282:H821-31.

Muruganandan S, Gupta S, Kataria M, Lal J, Gupta PK. Mangiferin protects the streptozotocin-induced oxidative damage to cardiac and renal tissues in rats. Toxicology 2002;176:165-73.

Jiang DJ, Tan GS, Ye F, Du YH, Xu KP, Li YJ, et al. Protective effects of xanthones against myocardial ischemia-reperfusion injury in rats. Acta Pharmacol Sin 2003;24:175-80.

Wexler BC. Myocardial infarction in young vs old male rats: Pathophysiologic changes. Am Heart J 1978;96:70-80.

Nirmala C, Puvanakrishnan R. Effect of curcumin on certain lysosomal hydrolases in isoproterenol-induced myocardial infarction in rats. Biochem Pharmacol 1996;51:47-51.

Dhawan S, Kapoor NK, Nityanand S. Effect of isoprenaline on lipid profile and cardiac enzymes in rats. Indian J Exp Biol 1978;16:376-8.

Banerjee SK, Maulik SK. Effect of garlic on cardiovascular disorders: A review. Nutr J 2002;1:4.

Decker RS, Poole AR, Griffin EE, Dingle JT, Wildenthal K. Altered distribution of lysosomal cathepsin D in ischemic myocardium. J Clin Invest 1977;59:911-21.

Ravichandran LV, Puvanakrishnan R, Joseph KT. Influence of isoproterenol-induced myocardial infarction on certain glycohydrolases and cathepsins in rats. Biochem Med Metab Biol 1991;45:6-15.

Mythily P, Devika PT. Cardioprotective effect of fisetin on lipid peroxide metabolism in isoproterenol induced myocardial infarction in male Wistar rats. Int J Innov Res Sci Eng Technol 2017;6:22306-14.

Sathish V, Ebenezar KK, Devaki T. Synergistic effect of nicorandil and amlodipine on lysosomal hydrolases during experimental myocardial infarction in rats. Biomed Pharmacother 2003;57:309-13.

Kawai Y, Anno K. Mucopolysaccharide-degrading enzymes from the liver of the squid, ommastrephes sloani pacificus. I. Hyaluronidase. Biochim Biophys Acta 1971;242:428-36.

Moore JC, Morris JE. A simple automated colorimetric method for determination of N-acetyl-beta-D-glucosaminidase. Ann Clin Biochem 1982;19:157-9.

Conchie J, Gelman AL, Levvy GA. Inhibition of glycosidases by aldonolactones of corresponding configuration. The C-4- and C-6-specificity of beta-glucosidase and beta-galactosidase. Biochem J 1967;103:609-15.

Sapolsky AI, Altman RD, Howell DS. Cathepsin D activity in normal and osteoarthritic human cartilage. Fed Proc 1973;32:1489-93.

Barrett AJ. A new assay for cathepsin B1 and other thiol proteinases. Anal Biochem 1972;47:280-93.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the folin phenol reagent. J Biol Chem 1951;193:265-75.

Salah AR. Cardioprotective efficacy of taurine on lipid-metabolism of isoproterenol-induced myocardial infarction. Int J Pharm Pharm Sci 2016;8:135-41.

Sudharsan PT, Mythili Y, Selvakumar E, Varalakshmi P. Lupeol and its ester ameliorate the cyclophosphamide provoked cardiac lysosomal damage studied in rat. Mol Cell Biochem 2006;282:23-9.

Garcia-Dorado D, Ruiz-Meana M. Propagation of cell death during myocardial reperfusion. News Physiol Sci 2000;15:326-30.

Kalra J, Prasad K. Oxygen free radicals and cardiac depression. Clin Biochem 1994;27:163-8.

Subashini R. Cardioprotective efficacy of Nelumbo nucifera Leaf extract on glycoprotein, membrane bound atpase and lysosomal enzymes against isoproterenol induced cardiotoxicity in Wistar rats. Int J Pharm Pharm Sci 2014;6:537-41.

Pillay CS, Elliott E, Dennison C. Endolysosomal proteolysis and its regulation. Biochem J 2002;363:417-29.

Devika PT, Prince PS. Preventive effect of (-)epigallocatechin-gallate (EGCG) on lysosomal enzymes in heart and subcellular fractions in isoproterenol-induced myocardial infarcted Wistar rats. Chem Biol Interact 2008;172:245-52.

Sano M, Takahashi Y, Yoshino K, Shimoi K, Nakamura Y, Tomita I, et al. Effect of tea (Camellia sinensis L.) on lipid peroxidation in rat liver and kidney: A comparison of green and black tea feeding. Biol Pharm Bull 1995;18:1006-8.

Ravens KG, Gudbjarnason S. Changes in the activities of lysosomal enzymes in infarcted canine heart muscle. Circ Res 1969;24:851-6.