EVALUATION OF THE HISTOPATHOLOGICAL AND BIOCHEMICAL EFFECT OF ALOE VERA AQUEOUS EXTRACT ON DIABETES MELLITUS INDUCED BY STREPTOZOTOCIN IN RATS
DOI:
https://doi.org/10.22159/ajpcr.2019.v12i6.30995Keywords:
Aloe Vera, Streptozotocin, Insulin, Antioxidants, Lipid peroxidationAbstract
Objective: Numerous plants have exciting pharmaceutical activities, but Aloe vera (AV) is likely to have many medicinal applications worldwide. Therefore, the present study focused on identifying the potential of AV aqueous extract to protect against complications of diabetes.
Methods: Fifty rats were divided into five groups. The first group, the normal group, received a vehicle solution. The second group, the AV group, received AV aqueous extract (1/2 ml/100 g body weight). The third group was the streptozotocin (STZ)-induced diabetic rat group (i.p. as a single dose), the fourth group was the ameliorative group (a week after STZ injection, rats were given the same daily oral dose of AV extract), and the fifth group was the protective group (rats were treated with the same dose of AV extract for 21 days, and on the 14th day after the start of AV treatment, STZ injection was performed). At the end of the experiment, changes in serum indices, such as insulin, glucose, aspartate transaminase (AST), alanine transaminase (ALT), urea and creatinine, and tissue antioxidants as well as histopathological alterations in the pancreas, liver, and kidney were evaluated.
Results: We found that STZ-treated animals displayed significant increases in tissue lipid peroxidation (LPO, as an indicator of oxidative stress), serum glucose, AST, ALT, urea, and creatinine, with a parallel decrease in the levels of serum insulin and tissue antioxidants. In accordance with these data, several histopathological alterations in the selected organs were observed. When diabetic animals received 1/2 ml/100 g body weight of AV extract, these deleterious effects were ameliorated.
Conclusion: In the current study, AV aqueous extract exhibited an ameliorative and protective effect against the oxidative damage and the associated complications that occurred in different rat organs due to diabetes mellitus induced by STZ.
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Salem MY, El-Azab N. The possible protective role of Aloe vera extracts in pancreatic β cells of experimentally induced diabetic rats: A histological and immunohistochemical study. Egypt J Histol 2014;37:571-8.
Szkudelski T. The mechanism of alloxan and streptozotocin action in B cells of the rat pancreas. Physiol Res 2001;50:537-46.
Gülçin I, Küfrevioglu OI, Oktay M, Büyükokuroglu ME. Antioxidant, antimicrobial, antiulcer and analgesic activities of nettle (Urtica dioica L). J Ethnopharmacol 2004;90:205-15.
Azemi ME, Namjoyan F, Khodayar MJ, Ahmadpour F, Darvish Padok A, Panahi M, et al. The antioxidant capacity and anti-diabetic effect of Boswellia serrata triana and planch aqueous extract in fertile female diabetic rats and the possible effects on reproduction and histological changes in the liver and kidneys. Jundishapur J Nat Pharm Prod 2012;7:168-75.
Rashid K, Das J, Sil PC. Taurine ameliorate alloxan induced oxidative stress and intrinsic apoptotic pathway in the hepatic tissue of diabetic rats. Food Chem Toxicol 2013;51:317-29.
Asbun J, Villarreal FJ. The pathogenesis of myocardial fibrosis in the setting of diabetic cardiomyopathy. J Am Coll Cardiol 2006;47:693 700.
Ha H, Lee HB. Oxidative stress in diabetic nephropathy: Basic and clinical information. Curr Diab Rep 2001;1:282-7.
Kashihara N, Haruna Y, Kondeti VK, Kanwar YS. Oxidative stress in diabetic nephropathy. Curr Med Chem 2010;17:4256-69.
Mavrogiannaki AN, Migdalis IN. Nonalcoholic fatty liver disease, diabetes mellitus and cardiovascular disease: Newer data. Int J Endocrinol 2013;2013:450639.
Hogan DB. Did osler suffer from paranoia antitherapeuticum baltimorensis? A comparative content analysis of the principles and practice of medicine and harrison’s principles of internal medicine, 11th edition. CMAJ 1999;161:842-5.
Nannipieri M, Gonzales C, Baldi S, Posadas R, Williams K, Haffner SM, et al. Liver enzymes, the metabolic syndrome, and incident diabetes: The Mexico City diabetes study. Diabetes Care 2005;28:1757-62.
Acharya JD, Ghaskadbi SS. Islets and their antioxidant defense. Islets 2010;2:225-35.
Tabatabaei SRF, Ghaderi S, Bahrami-Tapehebur M, Farbood Y, Rashno M. Aloe vera gel improves behavioral deficits and oxidative status in streptozotocin-induced diabetic rats. Biomed Pharmacother 2017;96:279-90.
Mohapatra S, Pradhan S, Rath B, Tripathy S. Antioxidant properties of Aloe vera in streptozotocin induced diabetic rats. Int J Pharm Bio Sci 2013;4:187-91.
Khan RA, Aslam M, Ahmed S. Evaluation of toxicological profile of a polyherbal formulation. Pharmacol Pharm 2016;7:56.
Surjushe A, Vasani R, Saple DG. Aloe vera: A short review. Indian J Dermatol 2008;53:163.
Hamman JH. Composition and applications of Aloe vera leaf gel. Molecules 2008;13:1599-616.
Helal EG, Hasan MH, Mustafa AM, Al-Kamel A. Effect of Aloe vera extract on some physiological parameters in diabetic albino rats. Egypt J Hosp Med 2003;12:53-61.
Rajasekaran S, Ravi K, Sivagnanam K, Subramanian S. Beneficial effects of Aloe vera leaf gel extract on lipid profile status in rats with streptozotocin diabetes. Clin Exp Pharmacol Physiol 2006;33:232-7.
Ramachandraiahgari Y, Madhavi R, Somesula SR, Adi PJ, Mannur IS, Enamala M, et al. Protective role of ethanolic extract of Aloe vera antioxidant properties on liver and kidney of streptozotocin-induced diabetic rats. Dig J Nanomater Biostruct 2012;7:175-84.
Manley SE, Stratton IM, Clark PM, Luzio SD. Comparison of 11 human insulin assays: Implications for clinical investigation and research. Clin Chem 2007;53:922-32.
Arun N, Nalini N. Efficacy of turmeric on blood sugar and polyol pathway in diabetic albino rats. Plant Foods Hum Nutr 2002;57:41-52.
Senaphan K, Kukongviriyapan U, Pakdeechote P, Kukongviriyapan V, Pannangpetch P. Rat model of a metabolic syndrome induced by a high-carbohydrate, high-fat diet with fructose in drinking water. Asia Pac J Sci Technol 2017;22:8.
Zawada RJ, Kwan P, Olszewski KL, Llinas M, Huang SG. Quantitative determination of urea concentrations in cell culture medium. Biochem Cell Biol 2009;87:541-4.
Han WK, Wagener G, Zhu Y, Wang S, Lee HT. Urinary biomarkers in the early detection of acute kidney injury after cardiac surgery. Clin J Am Soc Nephrol 2009;4:873-82.
Muralikrishna G, Nirmala M. Cereal α-amylases an overview. Carbohydr Polym 2005;60:163-73.
Hansen KB, Rosenkilde MM, Knop FK, Wellner N, Diep TA, Rehfeld JF, et al. 2-oleoyl glycerol is a GPR119 agonist and signals GLP-1 release in humans. J Clin Endocrinol Metab 2011;96:E1409-17.
Ekinci-Akdemir FN, Gülçin I, Gürsul C, Alwasel SH, Bayir Y. Effect of p-coumaric acid against oxidative stress induced by cisplatin in brain tissue of rats. J Anim Plant Sci 2017;27:1560-4.
Basu U, Good AG, Taylor GJ. Transgenic Brassica napus plants overexpressing aluminium-induced mitochondrial manganese superoxide dismutase cDNA are resistant to aluminium. Plant Cell Environ 2001;24:1269-78.
Sandalio LM, Dalurzo HC, Gómez M, Romero-Puertas MC, del Río LA. Cadmium-induced changes in the growth and oxidative metabolism of pea plants. J Exp Bot 2001;52:2115-26.
Alam MN, Bristi NJ, Rafiquzzaman M. Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharm J 2013;21:143 52.
Xia Y, Moody JB, Burton-Wurster N, Lust G. Quantitative in situ correlation between microscopic MRI and polarized light microscopy studies of articular cartilage. Osteoarthritis Cartilage 2001;9:393-406.
Lukić ML, Stosić-Grujicić S, Shahin A. Effector mechanisms in low-dose streptozotocin-induced diabetes. Dev Immunol 1998;6:119-28.
Mohamed J, Nazratun Nafizah AH, Zariyantey AH, Budin SB. Mechanisms of diabetes-induced liver damage: The role of oxidative stress and inflammation. Sultan Qaboos Univ Med J 2016;16:e132-41.
Pribac G, Craciun C, Szoke-Nagy T, Simeoni I, Lang C, Rosioru C, et al. Comparative ultrastructural study of pancreatic beta cells from diabetic rats treated with fenugreek seed flour or ganoderma flour. Ann Rom Soc Cell Biol 2011;16:62-80.
Yin J, Huang J, Chen C, Gao N, Wang F, Yu FS, et al. Corneal complications in streptozocin-induced type I diabetic rats. Invest Ophthalmol Vis Sci 2011;52:6589-96.
Noor A, Gunasekaran S, Manickam AS, Vijayalakshmi MA. Antidiabetic activity of Aloe vera and histology of organs in streptozotocin-induced diabetic rats. Curr Sci 2008;94:1070-6.
Kalailingam P, Balasubramanian K, Kannaian B, Mohammed AK, Meenakshisundram K, Tamilmani E, et al. Isolation and quantification of flavonoids from ethanol extract of Costus igneus rhizome (CiREE) and impact of CiREE on hypoglycaemic, electron microscopic studies of pancreas in streptozotocin (STZ)-induced diabetic rats. Biomed Prev Nutr 2013;3:285-97.
Kumar N, Kar A. Pyrroloquinoline quinone (PQQ) has potential to ameliorate streptozotocin-induced diabetes mellitus and oxidative stress in mice: A histopathological and biochemical study. Chem Biol Interact 2015;240:278-90.
Ou SY, Jackson GM, Jiao X, Chen J, Wu JZ, Huang XS, et al. Protection against oxidative stress in diabetic rats by wheat bran feruloyl oligosaccharides. J Agric Food Chem 2007;55:3191-5.
Furman BL. Streptozotocin-induced diabetic models in mice and rats. Curr Protoc Pharmacol 2015;70:5-47.
Weng YC, Chuang ST, Lin YC, Chuang CF, Chi TC, Chiu HL, et al. Caffeic acid phenylethyl amide protects against the metabolic consequences in diabetes mellitus induced by diet and streptozocin. Evid Based Complement Alternat Med 2012;2012:984780.
Gabriel NN, Qiang J, Ma XY, He J, Xu P, Liu K, et al. Dietary Aloe vera improves plasma lipid profile, antioxidant, and hepatoprotective enzyme activities in GIFT-tilapia (Oreochromis niloticus) after Streptococcus iniae challenge. Fish Physiol Biochem 2015;41:1321-32.
Goda M. Effect of dietary ginseng herb (Ginsana® G115) supplementation on growth, feed utilization, and hematological indices of nile tilapia, Oreochromis niloticus (L), fingerlings goda, Ashraf MA-S. J World Aquac Soc 2008;39:205-14.
Chen W, Van Wyk BE, Vermaak I, Viljoen AM. Cape aloes a review of the phytochemistry, pharmacology and commercialisation of Aloe ferox. Phytochem Lett 2012;5:1-12.
Rajasekaran S, Sivagnanam K, Subramanian S. Modulatory effects of Aloe vera leaf gel extract on oxidative stress in rats treated with streptozotocin. J Pharm Pharmacol 2005;57:241-6.
Nwajo HU. Antioxidant activity of the exudate from Aloe barbadensis leaves in diabetic rats. Biokemistri 2006;18:77-81.
Abo-Youssef AM, Messiha BA. Beneficial effects of Aloe vera in treatment of diabetes: Comparative in vivo and in vitro studies. Bull Fac Pharm Cairo Univ 2013;51:7-11.
Kumar GP, Arulselvan P, Kumar DS, Subramanian SP. Anti-diabetic activity of fruits of Terminalia chebula on streptozotocin induced diabetic rats. J Health Sci 2006;52:283-91.
Klein R. Hyperglycemia and microvascular and macrovascular disease in diabetes. Diabetes Care 1995;18:258-68.
Gupta BL, Nehal M, Baquer NZ. Effect of experimental diabetes on the activities of hexokinase, glucose-6-phosphate dehydrogenase and catecholamines in rat erythrocytes of different ages. Indian J Exp Biol 1997;35:792-5.
Kamble B, Gupta A, Moothedath I, Khatal L, Janrao S, Jadhav A, et al. Effects of Gymnema sylvestre extract on the pharmacokinetics and pharmacodynamics of glimepiride in streptozotocin induced diabetic rats. Chem Biol Interact 2016;245:30-8.
Simon-Giavarotti KA, Giavarotti L, Gomes LF, Lima AF, Veridiano AM, Garcia EA, et al. Enhancement of lindane-induced liver oxidative stress and hepatotoxicity by thyroid hormone is reduced by gadolinium chloride. Free Radic Res 2002;36:1033-9.
Subudhi U, Das K, Paital B, Bhanja S, Chainy GB. Alleviation of enhanced oxidative stress and oxygen consumption of L-thyroxine induced hyperthyroid rat liver mitochondria by Vitamin E and curcumin. Chem Biol Interact 2008;173:105-14.
Ugochukwu NH, Cobourne MK. Modification of renal oxidative stress and lipid peroxidation in streptozotocin-induced diabetic rats treated with extracts from Gongronema latifolium leaves. Clin Chim Acta 2003;336:73-81.
Kaleem M, Asif M, Ahmed QU, Bano B. Antidiabetic and antioxidant activity of Annona squamosa extract in streptozotocin-induced diabetic rats. Singapore Med J 2006;47:670-5.
Rajalingam R, Srinivasan N, Govindarajulu P. Effects of alloxan induced diabetes on lipid profiles in renal cortex and medulla of mature albino rats. Indian J Exp Biol 1993;31:577-9.
Khanra R, Bhattacharjee N, Dua TK, Nandy A, Saha A, Kalita J, et al. Taraxerol, a pentacyclic triterpenoid, from Abroma augusta leaf attenuates diabetic nephropathy in Type 2 diabetic rats. Biomed Pharmacother 2017;94:726-41.
Al-Logmani A, Zari T. Long-term effects of Nigella sativa L. Oil on some physiological parameters in normal and streptozotocin-induced diabetic rats. J Diabetes Mellit 2011;1:46.
Akpan UP, Nna VU, Ikpi DE, Osim EE, Antai AB. Effect of crude Aloe vera gel on serum enzymes, proteins and liver histology in alloxan-induced diabetic rats. Int J Sci Res 2012;3:114-9.
Tapiero H, Tew KD, Ba GN, Mathé G. Polyphenols: Do they play a role in the prevention of human pathologies? Biomed Pharmacother 2002;56:200-7.
Suleyman A, Gnanasekaran N, Daniel S. Amelioration of streptozotocininduced hyperglycemia and dyslipidemia through Aloe debrana. Int J Pharm Pharm Sci 2014;7:290-3.
Acharya N, Barai P, Katariya H, Acharya S, Santani D. Evaluation of antidiabetic potential of roots and stems of G. arborea. Int J Pharm Pharm Sci 2015;7:355-62.
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