RAPHANUS SATIVUS LINN. A NEW ANTINOCICEPTIVE FOR DIABETIC NEUROPATHY IN RATS DETERMINED BY RANDALL SELITTO APPROACH

SAMBIT KUMAR SAHOO; STHITAPRAGNYA PANDA

doi:10.22159/ajpcr.2019.v12i3.31003

Authors

SAMBIT KUMAR SAHOO Department of School of Pharmaceutical Sciences, SOA Deemed to be University, Bhubaneswar, Odisha, India. http://orcid.org/0000-0002-7867-3470
STHITAPRAGNYA PANDA Department of School of Pharmaceutical Sciences, SOA Deemed to be University, Bhubaneswar, Odisha, India.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i3.31003

Keywords:

Raphanus sativus linn, Streptozotozin, type II DM, sciatic nerve, antinociceptive effect, Randall selitto method

Abstract

Objective: The objective of the study was to evaluate the antinociceptive effect of Raphanus sativus Linn. using Randall Selitto method.

Methods: Streptozotocin, lard, casein, cholesterol, DL-methionine, yeast powder, quercetin, thiobarbituric acid, 2-nitrobenzoic acid (5, 5, Dithiobis), hematoxylin, and hydrogen peroxide were used. A diet rich in fat content was fed to the animals for a period of 2 weeks. After a stabilization period of 2weeks, the treatment period started and continued for a period of 8weeks. The nociceptive parameters were assessed once a week by Randall Selitto method and hot plate test. After treatment, the animals were sacrificed, and antioxidant parameters were assessed using sciatic nerve homogenate and histopathological analysis of sciatic nerve.

Results: Treatment R. sativus extract (RSE 100 mg/kg and 200 mg/kg) appreciably declined the levels of blood glucose in a dose-dependent manner, and it was comparable with standard quercetin. A significant increase in pain threshold levels was observed by the treatment RSE in hot plate method after the 4th week compared to diabetic control, and it was consistent until the end of treatment (p<0.01, p<0.001). In Randall Selitto method RSE produced a significant increase in paw withdrawal threshold after the 4th week compared to diabetic control, and it was consistently increased until the end of treatment. RSE (100 and 200 mg/kg) significantly restored the levels of antioxidant enzymes and decreased lipid peroxidation in a dose-dependent fashion in comparison with the diabetic control group. RSE (100 mg/kg and 200 mg/kg) attenuated the nerve degeneration and axonal swelling along with quercetin.

Conclusion: The findings from the current study showed the antinociceptive and antioxidant effect of R. sativus in neuropathic pain in diabetes.

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Author Biographies

SAMBIT KUMAR SAHOO, Department of School of Pharmaceutical Sciences, SOA Deemed to be University, Bhubaneswar, Odisha, India.

STHITAPRAGNYA PANDA , ASSISTANT PROFESSOR , department of pharmacology

STHITAPRAGNYA PANDA, Department of School of Pharmaceutical Sciences, SOA Deemed to be University, Bhubaneswar, Odisha, India.

m.pharm research scholar

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