Objectives: The present study was undertaken for investigating the protective effect of ethanolic extract of Mirabilis Jalapa Linn leaves on anti-tubercular drugs induced hepatotoxicity. Methods: Anti-tubercular drugs were used to induce hepatotoxicity. Ethanolic extract of leaves of Mirabilis Jalapa Linn (250 & 500 mg/kg p.o.) was administered with one hour prior administration of anti-tubercular drugs once daily for 35 days. Silymarin was used as standard drug (100 mg/kg p.o.). Results: Liver biomarkers such as SGOT, SGPT, ALP, TB, total cholesterol were elevated and total HDL were reduced on anti-tubercular drugs administration. Pretreatment of ethanolic extract of leaves of Mirabilis Jalapa Linn 250 mg/kg and 500 mg/kg with anti-tubercular drugs were significantly reduced liver biomarker enzymes. Antioxidant parameters such as SOD, CAT, GSH, GPx and GRx were suppressed and increased TBARs levels in anti-tubercular drugs administration but restored these antioxidant levels in pretreatment of ethanolic extract of Mirabilis Jalapa Linn leaves at a dose of 250 mg/kg and 500 mg/kg. Conclusion: The result of the present study was indicated that Mirabilis Jalapa Linn showed hepatoprotective effect on hepatotoxicity induced by anti-tubercular drugs.

Key Words: Hepatoprotective activity, Anti-tubercular drugs, Liver biomarkers, Mirabilis Jalapa, Antioxidant parameters.


Download data is not yet available.

Author Biography




Upadhyay G, Kumar A and Singh, MP. Effect of silymarin on pyrogallol and rifampicin induced hepatotoxicity in mouse. Eur. J. Pharmacol 2007; 565: 190-201.

Sodhi CP, Rana S, Attri S, Mehta S and Yaiphei K. Oxidative hepatic injury of isoniazid-rifampicin in young rats subjected to protein energy malnutrition. Drug Chem Toxicol 1998; 21 (3): 305-317.

Vipin Kumar, Pankaj K Modi and K K Saxena. Exploration of hepatoprotective activity of aqueous extract of Tinospora Cordifolia an experimental study. Asian journal of pharmaceutical and clinical research 2013, Vol 6, Issue 1, pp: 87-91.

Anurag Jain, I P Jain, SP Singh and Asha Agarwal. To evaluate hepatoprotective activity of roots of Cynodon Dactylon an experimental study. Asian journal of pharmaceutical and clinical research 2013, Vol 6, Issue 2, pp: 109-112.

Daniel M. Medicinal plants chemistry and properties. Science Publishers, 2006, Enfield, NH, U.S.A. pp.107.

Holdsworth D, K. A preliminary study of medicinal plants of Easter Island, South Pacific. Int J Pharmacognosy 1992; 30: 27-32.

Oladunmoye MK. Comparative Evaluation of Antimicrobial Activities of Leaf Extract of Mirabilis jalapa. Trends Med Res 2007; 2 (2):108-112.

Yang SW. Three new phenolic compounds from a manipulated plant cell culture, Mirabilis jalapa. J Nat Prod 2001; 64: 313-317.

DeBolle M, Osborn R, Goderis I, Noe L, Acland D and Hart C. Antimicrobial peptides from Mirabilis jalapa and Amaranth caudatus: expression, processing, localization and biological activity in transgenic tobacco†Plant, Mol Biol Rep 1996; 31: 993–1008.

Wang, R. N. Characterization of Mirabilis antiviral protein—a ribosome inactivating protein from Mirabilis jalapa L. Biochem. Int 1992; 28(4): 585–93.

Subin Mary Zachariah, Dr. N A Aleykutty, Vidya Viswanad, Somu Jacob and Visakh Prabhakar. Invitro antioxidant potential of methanolic extract of Mirabilis Jalapa. Free Rad Antiox 2011, vol.1, Issue 4, Oct-Dec: pg 82-86.

OECD guidelines for the testing of chemicals revised draft guideline 423: acute oral toxicity: Paris: OECD: 2000.

M.N.Ghosh. Fundamentals of experimental pharmacology, 2007.

Kakkar P, Das B and Viswanathan PN. A modified spectrophotometric assay of SOD. Indian Journal of Biochemistry Biophysics 1984; 2: 130-132.

Aebi H. Catalase in vitro. Methods in Enzymology 1984: 105: 121-126.

Sener G, Sehirli AO, Gedik N and Dulger GA. Rosiglitazone, a PPAR-γ ligand, protects against burn-induced oxidative injury of remote organs. Burns 2007; 33: 587-593.

Paglia DE and Valentine WN. Studies on the quantitative and qualitative characterization of erythrocytes glutathione peroxidase. J. Lab. Clin. Med 1986; 40: 158-69.

Racker E. Glutathione reductase from bakers’ yeast and beef liver. The Journal of Biological Chemistry 1955; 217: 855-866.

Rukkumani R, Aruna K, Varma PS, Rajasekaran KN and Menon VP. Comparative effects of curcumin and an analog of curcumin on alcohol and PUFA induced oxidative stress. Journal of Pharmacy & Pharmaceutical Sciences 2004; 7: 274-283.

Vijaya Padma V, Suja R and Shyamala Devi CS. Hepatoprotetive effect of Liv.52 on Antitubercular Drug induced Hepatotoxicity in rats, Fitoterapia (LXIX) 1998; 6: 520.

Saraswathy SD, Suja V, Gurumurthy and Shyamala devi C S. Effect of Liv.100 against anti-tuberculosis drugs (isoniazid, rifampicin and pyrazinamide) induced hepatotoxicity in rats. Indian J Pharmacol, 1998, 30:233.

Man-Fung Y, Takanobu K, Masashi M et al. Clinical outcome and virologic profiles of severe hepatitis B exacerbation due to YMDD mutations. J Hepatol 2003; 39: 850-855.

Kissler H J, Hauffen J, Hennig R, Gepp H and Schwille PO. Glucose and lipid metabolism after liver transplantation in inbred rats: Consequences of hepatic denervation, Metabolism 2005; 54: 881-890.

Viswanatha Swamy A H M, Rucha V. Kulakarni, A.H.M. Thippeswamy, B.C.Koti and Aparna Gore. Evaluation of hepatoprotective activity of Cissus quadrangularis stem extract against isoniazid induced liver damage in rats. Indian journal of pharmacology 2010, Dec, Vol 42, Issue 6: 397-400.

Attri S, Rana SV, Vaiphei K, Sodhi CP, Katyai R, Goel RC et al. Isoniazid and rifampicin induced oxidative hepatic injury protection by N-acetylcysteine. Hum Exp Toxicol 2000; 19: 517-24.



How to Cite

JYOTHI , B., S. MOHANALAKSHMI, and A. K. “PROTECTIVE EFFECT OF MIRABILIS JALAPA LEAVES ON ANTI-TUBERCULAR DRUGS INDUCED HEPATOTOXICITY”. Asian Journal of Pharmaceutical and Clinical Research, vol. 6, no. 7, Aug. 2013, pp. 221-4,