IN VITRO PHARMACOLOGICAL SCREENING METHODS FOR ANTI-INFLAMMATORY AGENTS
DOI:
https://doi.org/10.22159/ajpcr.2018.v11s4.31709Keywords:
Reactive oxygen species, Polymorphonuclear cells, Cytokines, Cyclooxygenase, Macrophages, NeutrophilAbstract
Context: The development of in vitro methods is important in drug discovery process due to the restriction of animal use in the pharmacological screening process.
Objective: The objective of the review was to understand the in vitro pharmacological screening methods for anti-inflammatory agents.
Methods: The present review details various in vitro methods available for the screening of activity of therapeutic agents against inflammation.
Results: Inhibition and measurement of nitric oxide production, mast cell degranulation, leukocyte adhesion assay, platelet-neutrophil adhesion assay, lipopolysaccharide-induced response assay, N-formyl-methionyl-leucyl-phenylalanine-induced O2 generation by polymorphonuclear cells, and cyclooxygenase assays are discussed in this review.
Conclusions: These methods help to understand the real mechanism of inflammation and mediators involved in this process and help to
identify new compounds possessing the anti-inflammatory activity.
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