DEVELOPMENT OF ANALYTICAL METHOD FOR IDENTIFICATION OF ESSENTIAL OIL FROM BLUMEA ERIANTHA DC IN PLASMA.
Abstract
Objective
The present study was designed to develop a head space gas chromatography method using mass spectrometric detection for identification of essential oil constituents from Blumea eriantha DC in plasma.
Methods
The essential oil was extracted by hydrodistillation using Clevenger type apparatus. The rabbit plasma samples were spiked with essential oil and were further analyzed using dynamic head-space sampling with gas chromatography-mass spectrometry (GC-MS).
Results
The analysis of plasma samples spiked with essential oil has revealed the selective absorption of some of the essential oil components.
Conclusion
Analytical method has been developed for identification of essential oil constituents in plasma which will be helpful in further in vivo pharmacokinetic studies of the essential oil.
KEYWORDS
Blumea eriantha DC, Dynamic Headspace Sampling, GC-MS, Plasma.
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References
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