ANTI-INFLAMMATORY ACTIVITY OF METHANOL EXTRACT OF NIEBUHRIA APETALA (ROTH) DUNN – IN VITRO MODELS: Inflammatory property of Niebuhria apetala

RAJESH A; DOSS A; TRESINA PS; MOHAN VR

doi:10.22159/ajpcr.2019.v12i5.32512

Authors

RAJESH A Department of Medicinal Botany, Government Siddha Medical College, Palyamkottai, Tamil Nadu, India.
DOSS A Department of Botany, V. O. Chidambaram College, Tuticorin, Tamil Nadu, India.
TRESINA PS Department of Botany, V. O. Chidambaram College, Tuticorin, Tamil Nadu, India.
MOHAN VR Department of Biomedical Science and Technology, Noorul Islam Centre for Higher Education, Kumaracoil, Tamil Nadu,

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i5.32512

Keywords:

Niebuhria apetala, Hypotonic, HRBC, Antilipoxygenase

Abstract

Objective: The objective of this study was to determine the anti-inflammatory activity of methanol extract of Niebuhria apetala and its possible mechanism of action.

Methods: Methanol extract of Niebuhria apetala leaf (NAL) was assessed for its anti-inflammatory activity by in vitro methods. Using albumin denaturation assay, proteinase inhibitory activity, membrane stabilization, and antilipoxygenase activity at different concentrations, in vitro anti-inflammatory activity was estimated. The standard drug used for this purpose was aspirin.

Results: Methanol extract NAL at a concentration range of 100–500 μg/ml significant (p<0.01) protects the heat-induced protein denaturation. At the concentration of 500 mg/ml, NAL showed significant (p<0.01) inhibition of protease inhibitory action. Heat-induced hemolysis of erythrocyte, hypotonicity-induced hemolysis, and lipooxygenase activity were significant (p<0.01) inhibited at the concentration of 500 μg/ml.

Conclusion: Finally, the present study indicates that methanol extract of Niebuhria apetala can be a potential source of anti-inflammatory agent.

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References

Prabhu VV, Guruvayoorappan C. Anti-inflammatory and anti-tumor activity of the marine mangrove Rhizophora apiculata. J Immunotoxicol 2012;9:341-52.

Rajendran V, Lakshmi KS. In vitro and in vivo anti-inflammatory activity of leaves of Symplocos cochinchnensis (Lour) Moore ssp Laurina. Bangladesh J Pharmacol 2008;3:121-4.

Yasmine Y, Caroline Jeba R. In vitro cytotoxicity activity of hydroalcoholic extract of polyherbal Churnam (Cofri) in normal liver and kidney cell lines. Asian J Pharm Clin Res 2019;12:74-7.

Supriya J, Kishor G, Aniket G. Phytochemical screening and antimicrobial activity of Portulaca quadrifida Linn. Asian J Pharm Clin Res 2019;12:78-81.

Ambati RR, Phang SM, Ravi S, Aswathanarayana RG. Astaxanthin: Sources, extraction, stability, biological activities and its commercial applications a review. Mar Drugs 2014;12:128-52.

Mizushima Y, Kobayashi M. Interaction of anti-inflammatory drugs with serum proteins, especially with some biologically active proteins. J Pharm Pharmacol 1968;20:169-73.

Sakat S, Juvekar AR, Gambhire MN. In vitro antioxidant and anti-inflammatory activity of methanol extract of Oxalis corniculata Linn. Int J Pharma and Pharmacol Sci 2010;2:146-55.

Oyedepo OO, Femurewa AJ. Anti-protease and membrane stabilizing activities of extracts of fagra zanthoxiloides, Olax subscorpioides and Tetrapleura tetraptera. Int J of Pharmacong 1995;33:65-9.

Sadique J, Al-Rqobahs WA, Bughaith, EI-Gindi Ar. The bioactivity of certain medicinal plants on the stabilization of RBS membrane system. Fitoterapia 1989;60:525-32.

Shinde UA, Kulkarni KR, Phadke AS, Nair AM, Mungantiwar AA, Dikshit VJ, et al. Mast cell stabilizing and lipoxygenase inhibitory activity of Cedrus deodara (Roxb.) loud. Wood oil. Ind J Exp Biol 1999;37:258-61.

Azeem AK, Dilip C, Prasanth SS, Junise V, Shahima H. Anti-inflammatory activity of the glandular extracts of Thunnus alalunga. Asia Pac J Med 2010;3:412-20.

Das SN, Chatterjee S. Long term toxicity study of ART-400. Ind Indg Med 1995;16:117-23.

Hess SM, Milloning RC. In: Lepow LH, Wards PA, editors. Inflammation, Mechanism and Control. New York: Academic Press; 1972. p. 1-72.

Gardner HW. Recent investigations into the lipoxygenase pathway of plants. Biochim Biophys Acta 1991;1084:221-39.

Abad MJ, Bermejo P, Villar A. The activity of flavonoids extracted from Tanacetum microphyllum DC. (Compositae) on soybean lipoxygenase and prostaglandin synthetase. Gen Pharmacol 1995;26:815-9.