IN VITRO ANTIOXIDANT AND ANTICANCER ACTIVITIES OF ETHANOLIC FRUITS EXTRACT OF Ziziphus jujube Mill.
DOI:
https://doi.org/10.22159/ajpcr.2019.v12i6.32753Keywords:
Anticancer activity,, Antioxidant activity,, Ziziphus jujubeAbstract
Objective: Antioxidant and anticancer studies of the ethanolic fruit extract of Ziziphus jujuba were aimed at detecting the phytochemicals and ascertain the antioxidant and anticancer activities using in vitro experimental models.
Materials and Methods: In this study, physiochemical parameters were carried out, and the content of phytoorganic constituents such as total carbohydrates, fats, proteins, crude fibers, and flavonoids was determined. Liquid chromatography–mass spectrometry (LC–MS) has been used for detecting the phytochemicals, and 10 different concentrations have been screened for their antioxidant and anticancer activities.
Results: Ethanolic fruit extract of Z. jujuba revealed the presence of caffeic acid hexoside dimer, chlorogenic acid, and triterpenoid derivative by LC–MS analysis. The extract exhibited the maximum antioxidant activity of 25.32% at 10 mg/ml in 2,2-diphenyl-1-picrylhydrazyl free radical scavenging assays, which are comparable to that of standard butylated hydroxytoluene. High concentration (10 mg/ml) of the extract revealed anticancer efficacy up to 40.36% by MTT assay, which is equivalent to the action of 5-flurouracil.
Conclusion: The ethanolic fruit extract of Z. jujuba might possess antioxidant and anticancer activities owing to the occurrence of bioactive compounds.
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