PHYSICAL AND CHEMICAL CHARACTERIZATION OF GREEN SYNTHESIZED SILVER NANOPARTICLES USING STEM OF HIBISCUS VITIFOLIUS L. AND ITS ANTIMICROBIAL AND ANTIOXIDANT POTENTIAL

ANTONY LAWRENCE A; THOMAS JOSEPH PRAKASH J

doi:10.22159/ajpcr.2019.v12i6.33113

Authors

ANTONY LAWRENCE A Department of Physics, Government Arts College (Affiliated to Bharathidasan University), Tiruchirappalli, Tamil Nadu, India.
THOMAS JOSEPH PRAKASH J Department of Physics, Government Arts College (Affiliated to Bharathidasan University), Tiruchirappalli, Tamil Nadu, India.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i6.33113

Keywords:

Antimicrobial activity,, Antioxidant assay,, Characterization,, Hibiscus vitifolius L,, Silver nanoparticles

Abstract

Objectives: The aim of our work was to synthesize the silver nanoparticle (AgNP) using Hibiscus vitifolius L. stem extract its characterization and evaluation of antimicrobial and antioxidant assay.

Materials and Methods: The silver nitrate (1 mM) mixed with aqueous stem extract of H. vitifolius L. after the nanoparticles is examined by Fourier-transform infrared (FT-IR), ultraviolet-visible (UV-vis) spectroscopy, field emission scanning electron microscopy (FE-SEM), energy-dispersive X-ray (EDAX), X-ray powder diffraction (XRD), dynamic light scattering (DLS), zeta potential, thermogravimetry/differential thermal analysis (TG/DTA), and differential scanning calorimetry (DSC). The aqueous stem extract is examined for phytochemical screening, gas chromatography–mass spectrometry (GC–MS) analysis, FT-IR, and UV-vis spectroscopy. The antibacterial, antifungal, and antioxidant assay were also evaluated for the AgNPs.

Results: The aqueous stem extract shows 20 compounds in GC–MS analysis. The FT-IR and UV-vis spectroscopy show the biocompounds. H. vitifolius stem extract-AgNPs (HVS-AgNPs) examined in UV and FT-IR shows the presence of AgNPs, FE-SEM shows that the particle size is 30–70 nm, EDAX shows the presence of silver metal, and XRD shows that the particles are face-centered cubic. DLS shows the hydrodynamic size 136.9 nm, zeta potential shows the stability (−18.6 mV), and TG/DTA and DSC show that the particles are stable up to 335°C. The HVS-AgNPs are also evaluated in antimicrobial and antioxidant potential and the report shows a good inhibition.

Conclusion: The stem extract of H. vitifolius L. can be used for green synthesis of AgNPs and could be used as antimicrobial and antioxidant potential.

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Author Biographies

ANTONY LAWRENCE A, Department of Physics, Government Arts College (Affiliated to Bharathidasan University), Tiruchirappalli, Tamil Nadu, India.

PG & Research Department of Physics, Government Arts College (Affiliated to Bharathidasan University), Trichy-620022.

THOMAS JOSEPH PRAKASH J, Department of Physics, Government Arts College (Affiliated to Bharathidasan University), Tiruchirappalli, Tamil Nadu, India.

PG& Research Department of Physics,Government Arts College,Trichy-22.

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