EXPRESSION OF CANCER STEM CELL MARKER OCTAMER-BINDING TRANSCRIPTION FACTOR 4 IN HIGH-GRADE TRANSITIONAL CELL CARCINOMA

MUROOJ JASSIM MOHAMMED; BASIM SHEHAB AHMED

doi:10.22159/ajpcr.2019.v12i6.33189

Authors

MUROOJ JASSIM MOHAMMED Department of Pathology and Forensic Medicine, College of Medicine, Mustansiriyah University, Baghdad, Iraq.
BASIM SHEHAB AHMED Department of Pathology and Forensic Medicine, College of Medicine, Mustansiriyah University, Baghdad, Iraq.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i6.33189

Keywords:

Octamer-binding transcription factor 4,, Transitional cell carcinoma,, Bladder

Abstract

Objectives: The objectives of this study were to provide an outlook of urothelial carcinoma through the immunohistochemical expression patterns of octamer-binding transcription factor 4 (OCT4) in high-grade transitional cell carcinoma (TCC) of the urinary bladder.

Methods: A total number of 60 tissue samples were collected for the study. Patients were divided into two groups according to the pathological diagnosis of the bladder tissue, Group A: 30 cases with high-grade TCC of the bladder and Group B: 30 cases with apparently normal bladder tissue. Tissue immunohistochemical analysis was applied to investigate the expression patterns of cancer stem cell (CSC) markers OCT4 in bladder samples.

Results: OCT4 was positive in 80% of specimens of Group A and 3.3% in specimens of Group B. The association between OCT4 marker result and certain histopathological features in high-grade group: Positive OCT4 result was found in patients with inflammation and necrosis (90.9%) with a significant association (p=0.013). Regarding muscular invasion, we noticed that 87.5% of patients with muscular invasion showed positive OCT4 marker result with a significant association (p=0.039) between OCT4 marker result and muscular invasion. As well, OCT4 marker was highly sensitive and specific (sensitivity=66.7%, specificity=96.7%, and accuracy=76.7%).

Conclusion: There was a significant expression of CSC OCT4 in high-grade TCC, OCT4 can be considered as a key regulator of tumor progression, aggressive behavior, and metastasis.

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