ANTIOXIDANT ACTIVITY OF PLANT PARTS EXTRACTS FROM STERCULIA QUADRIFIDA R. BR.
DOI:
https://doi.org/10.22159/ajpcr.2019.v12i7.33261Keywords:
Sterculia quadrifida,, Faloak,, Antioxidant,, Regrown barkAbstract
Objective: Sterculia quadrifida R. Br. of Sterculiaceae family is locally known as “Faloak” in East Nusa Tenggara Province, Indonesia. S. quadrifida is used in folk medicine to treat hepatitis, rheumatism, and to recover stamina. The aim of this study was to determine the total flavonoids, total phenolic content (TPC), and antioxidant activity of extracts from different plant parts of S. quadrifida.
Methods: The sampled parts of S. quadrifida were non-stripped stem bark, new regrown stem bark, old regrown stem bark, root bark, branch bark, and leaves. Stem bark was classified into three categories, namely, bark that has never been peeled (non-stripped stem bark), old regrown stem bark (estimated to be >6 months after debarking), and new regrown stem bark (estimated to be <6 months after debarking). Total flavonoid content (TFC) was determined by colorimetric aluminum chloride method and TPC was measured using Folin–Ciocalteu’s reagent. Antioxidant activity was determined with 2,2-diphenyl-1-picrylhydrazyl (DPPH).
Results: The concentrations of flavonoids in S. quadrifida extracts from different plant parts varied from 0.58±0.13 to 1.25±0.10 mg QE/g. The TPC in the extracts of different plant parts ranged between 8.61±0.09 and 10.43±0.08 mg GAE/g. Branch bark has the highest total flavonoid and phenolic content. The extract of new regrown stem bark exhibited potent antioxidant activity with inhibitory concentration (IC50) values of 2.51±0.03 μg/ml.
Conclusion: This study demonstrated for the first time that extracts from different plant parts of S. quadrifida exhibited strong antioxidant activity. However, the total phenolic and flavonoid contents in S. quadrifida only indicated a weak correlation with its antioxidant activity.
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Siswadi S, Raharjo AS, Pudjiono E, Saragih GS, Rianawati H. Utilization of faloak (Sterculia quadrifida R.Br.) bark as remedy in Timor Island. In: Njurumana G, Rahardjo SA, Riwu Kaho M, Kurniawan H, Hidayatullah M, editors. Seminar Nasional Biodiversitas Savana Nusa Tenggara. Kupang, Indonesia: Balai Penelitian dan Pengembangan Lingkungan Hidup dan Kehutanan Kupang; 2016.
Siswadi S, Rianawati H, Saragih GS, Sulistyo DH. The potency of faloak’s (Sterculia quadrifida, R.Br.) active compunds as natural remedy. In: Rizal M, Januawati NM, Widyastuti Y, Brotokardono L, Efendi R, Rohadi D, et al., editors. International Seminar ‘Forests and Medicinal Plants for Better Human Welfare. Bogor, Indonesia: Center for Forest Productivity Research and Development; 2014. p. 73-9.
Siswadi S, Saragih GS. Acute tocixity of Sterculia quadrifida R. Br bark ethanol extract on sprague-dawley rats. Trad Med J 2018;23:127-34.
Akter K, Barnes EC, Brophy JJ, Harrington D, Community Elders Y, Vemulpad SR, et al. Phytochemical profile and antibacterial and antioxidant activities of medicinal plants used by aboriginal people of New South Wales, Australia. Evid Based Complement Alternat Med 2016;2016:4683059.
Shanthi P, Tamilorasan G, Anitha K, Karthikeyan S. Film and pore diffusion modeling for adsorption of reactive red-4 onto Sterculia quadrifida seed shell waste as activated carbon. Rasayan J Chem 2014;7:229-40.
Lassak EV, McCarthy T. Australian Medicinal Plants. New South Wales: Methuen Australia; 1983.
Delvaux C, Sinsin B, Darchambeau F, Van Damme P. Recovery from bark harvesting of 12 medicinal tree species in Benin, West Africa. J Appl Ecol 2009;46:703-12.
Amin A, Wunas J, Anin YM. Determination of antioxidant activity of ethanolic extract of faloak (Sterculia quadrifida R. Br.) using DPPH (2, 2-diphenyl-1-picrylhydrazyl). J Fito Ind 2015;2:111-4.
Lulan TY, Fatmawati S, Santoso M, Ersam T. Antioxidant capacity of some selected medicinal plants in East Nusa Tenggara, Indonesia: The potential of Sterculia quadrifida R. Br. Free Radic Ant 2018;8:96-101.
Harborne JB. Phytochemical Methods. Bandung: Penerbit ITB; 1987. p. 78.
Govind P. Medicinal plants against liver diseases. Int Res J Pharm 2011;2:115-21.
Yadav A, Kumari R, Yadav A, Mishra J, Srivatva S, Prabha S. Antioxidants and its functions in human body – A review. Res Environ Life Sci 2016;9:1328-31.
Vinson JA, Su X, Zubik L, Bose P. Phenol antioxidant quantity and quality in foods: Fruits. J Agric Food Chem 2001;49:5315-21.
Molan A, Ismail M, Nsaif R. Phenolic contents and antioxidant activity of peels and seeds of orange (Citrus sinensis) cultivated in Iraq. World J Pharm Pharm Sci 2016;5:473-82.
Lagha-Benamrouche S, Madani K. Phenolic contents and antioxidant activity of orange varieties Citrus sinensis L. and Citrus aurantium L. cultivated in Algeria: Peels and leaves. Ind Crop Prod 2013;50:723-30.
Othman A, Mukhtar NJ, Ismail NS, Chang SK. Phenolics, flavonoids content and antioxidant activities of 4 Malaysian herbal plants. Int Food Res J 2014;21:759.
Statistic Bureau of East Nusa Tenggara Province. Kupang city in 2015 figures. Kupang; 2016.
Siswadi S. Rendemen and Total Flavonoid Content of Faloak (Sterculia quadrifida R.Br.) Bark from Different Diameter Class and Altitude. Yogyakarta: Universitas Gadjah Mada; 2015.
Pękal A, Pyrzynska K. Evaluation of aluminium complexation reaction for flavonoid content assay. Food Anal Methods 2014;7:1776-82.
Chaovanalikit A, Wrolstad R. Total anthocyanins and total phenolics of fresh and processed cherries and their antioxidant properties. J Food Sci 2004;69:FCT67-72.
Marxen K, Vanselow KH, Lippemeier S, Hintze R, Ruser A, Hansen UP. Determination of DPPH radical oxidation caused by methanolic extracts of some microalgal species by linear regression analysis of spectrophotometric measurements. Sensors (Basel) 2007;7:2080-95.
Sasidharan S, Chen Y, Saravanan D, Sundram KM, Yoga Latha L. Extraction, isolation and characterization of bioactive compounds from plants’ extracts. Afr J Tradit Complement Altern Med 2011;8:1-10.
Syamsudin SR, Simanjuntak P. Antidiabetic activity of active fractions of Leucaena leucocephala (lmk) Dewit seeds in experiment model. Eur J Sci Res 2010;43:384-91.
Saranya D, Sekar J, Adaikala RG. Assessment of antioxidant activities, phenol and flavonoid contents of different extracts of leaves, bark and root from the Abutilon indicum (l.) sweet. Asian J Pharm Clin Res 2017;10:88-94.
Dewatisari WF, Rumiyanti L, Rakhmawati I. Rendemen and phytochemical screening of Sanseviera sp. leaf. J Pen Perta Terp 2018;17:197-202.
Olajuyigbe OO, Afolayan AJ. Phenolic content and antioxidant property of the bark extracts of Ziziphus mucronata willd. subsp. mucronata willd. BMC Complement Altern Med 2011;11:130.
Tsai K, Lin B, Perng D, Wei J, Yu Y, Cherng JM. Immunomodulatory effects of aqueous extract of Ocimum basilicum Linn. and some of its constituents on human immune cells. J Med Plant Res 2011;5:1873-83.
Stankovic MS, Niciforovic N, Mihailovic V, Topuzovic M, Solujic S. Antioxidant activity, total phenolic content and flavonoid concentrations of different plant parts of Teucrium polium L. subsp. polium. Acta Soc Bot Pol 2012;81:117-22.
del Baño MJ, Lorente J, Castillo J, Benavente-García O, del Río JA, Ortuño A, et al. Phenolic diterpenes, flavones, and rosmarinic acid distribution during the development of leaves, flowers, stems, and roots of Rosmarinus officinalis. Antioxidant activity. J Agric Food Chem 2003;51:4247-53.
Sampaio BL, Edrada-Ebel R, Da Costa FB. Effect of the environment on the secondary metabolic profile of Tithonia diversifolia: A model for environmental metabolomics of plants. Sci Rep 2016;6:29265.
Rajendran V. In vitro antiproliferative effect, cytotoxicity and apoptosis study of biogenic silver nanoparticles synthesized using Sterculia quadrifida leaf extract. J Eng Appl Sci 2018;13:1414-20.
Mustafa R, Hamid AA, Mohamed S, Bakar FA. Total phenolic compounds, flavonoids, and radical scavenging activity of 21 selected tropical plants. J Food Sci 2010;75:C28-35.
Mashkor A. Phenolic content and antioxidant activity of fenugreek seeds extract. Int J Pharmacogn Phytochem Res 2014;6:841-4.
Fasola T, Akinloye A, Tossou M, Akoegninou A. The phytochemical and structural make-up of regrown and original tree barks used in ethnomedicine. World J Agric Res 2013;9:92-8.
Zuraida Z, Sulistiyani S, Sajuthi D, Suparto IH. Phenol, flavonoid and antioxidant activity of pulai (Alstonia scholaris R. br) bark extract. J Penelit Has Hutan 2017;35:211-9.
Nimmi O, George P. Evaluation of the antioxidant potential of a newly developed polyherbal formulation for antiobesity. Int J Pharm Pharm Sci 2012;4:505-10.
Le Son H, Anh NP. Phytochemical composition, in vitro antioxidant and anticancer activities of quercetin from methanol extract of Asparagus cochinchinensis (Lour.) Merr. Tuber. J Med Plant Res 2013;7:3360-6.
Heo BG, Park YJ, Park YS, Bae JH, Cho JY, Park K, et al. Anticancer and antioxidant effects of extracts from different parts of indigo plant. Ind Crop Prod 2014;56:9-16.
Phongpaichit S, Nikom J, Rungjindamai N, Sakayaroj J, Hutadilok-Towatana N, Rukachaisirikul V, et al. Biological activities of extracts from endophytic fungi isolated from Garcinia plants. FEMS Immunol Med Microbiol 2007;51:517-25.
Jacob J, Sreejith K. Antioxidant and anti-inflammatory properties of Pterospermum rubiginosum heyne ex wight and arn and Pterospermum reticulatum wight and Arn (Sterculiaceae): An in vitro comparative study. Asian J Pharm Clin Res 2019;12:272-5.
Nndwammbi M, Ligavha-Mbelengwa M, Anokwuru C, Ramaite I. The effects of seasonal debarking on physical structure, polyphenolic content and antibacterial and antioxidant activities of Sclerocarya birrea in the Nylsvley nature reserve. S Afr J Bot 2018;118:138-43.
Jain A, Sinha P, Jain A, Vavilala S. Estimation of flavonoid content, polyphenolic content and antioxidant potential of different parts of Abrus precatorius (L.). Int J Pharm Pharm Sci 2015;7:157-63.
Harish R, Shivanandappa T. Antioxidant activity and hepatoprotective potential of Phyllanthus niruri. Food Chem 2006;95:180-5.
Sulistiany H, Sudirman LI, Dharmaputra OS. Production of fruiting body and antioxidant activity of wild Pleurotus. HAYATI J Biosci 2016;23:191-5.
Widyawati PS, Wijaya CH, Hardjosworo PS, Sajuthi D. Volatile compounds of Pluchea indica Less and Ocimum basillicum Linn essential oiland potency as antioxidant. HAYATI J Biosci 2013;20:117-26.
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