TOTAL PHENOLIC CONTENT AND FREE RADICAL SCAVENGING ACTIVITY OF MIMUSOPS ELENGI L. LEAVES

VINEETA SINGH; PANDEY VN

doi:10.22159/ajpcr.2019.v12i6.33282

Authors

VINEETA SINGH Department of Botany, Experimental Botany and Nutraceutical Laboratory, Deen Dayal Upadhyay Gorakhpur University, Gorakhpur, Uttar Pradesh, India.
PANDEY VN Department of Botany, Experimental Botany and Nutraceutical Laboratory, Deen Dayal Upadhyay Gorakhpur University, Gorakhpur, Uttar Pradesh, India.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i6.33282

Keywords:

Mimusops elengi L,, Total phenolic content,, Free radical scavenging activity,, 2,2-diphenyl-1-picrylhydrazyl assay,, Nil

Abstract

Objective: The motive of the current study was to find out the total phenolic content (TPC) and free radical scavenging (FRS) activity of various solvent extracts of leaves.

Methods: Leaves powder was subjected to extraction with different solvents, namely methanol, methanol 80%, ethanol, ethanol 80%, chloroform, acetone, aqueous, and ethyl acetate. Different concentrations of extracts were evaluated for FRS activity by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay and TPC spectrophotometrically by Folin–Ciocalteu method.

Results: The results clearly indicate that inhibition concentration of DPPH by leaf extracts was higher in methanol (inhibitory concentration 50=8.74) followed by ethanol >ethanol 80% >methanol 80% > acetone > aqueous > chloroform > ethyl acetate. TPC was higher in methanol (6.2±0.01 mg gallic acid equivalent/g) followed by ethanol > methanol 80% > acetone > aqueous >ethyl acetate > ethanol 80% > chloroform. A significant correlation (y=0.6265x +42.91 R2=0.9756) exists between TPC and FRS activity of methanolic extract.

Conclusion: The finding revealed that methanolic extract of leaves contains a significant amount of FRS activity and phenolic compounds compared to other extracts.

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Author Biography

VINEETA SINGH, Department of Botany, Experimental Botany and Nutraceutical Laboratory, Deen Dayal Upadhyay Gorakhpur University, Gorakhpur, Uttar Pradesh, India.

Professor of Botany,

Department of Botany,
DDU Gorakhpur University,
Gorakhpur - 273009, (U.P.) India

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