IN VITRO GROWTH INHIBITORY EFFECT OF ZnO NANOPARTICLES ON HUMAN LIVER CANCER CELL LINES (Huh7)

ANANTHALAKSHMI R; XAVIER RAJARATHINAM SR; Mohamed Sadiq A; MOHAMED SADIQ A

doi:10.22159/ajpcr.2019.v12i6.33486

Authors

ANANTHALAKSHMI R Department of Biochemistry, Sacred Heart College, Tirupattur, Vellore, Tamil Nadu, India.
XAVIER RAJARATHINAM SR Department of Chemistry, Sacred Heart College, Tirupattur, Vellore, Tamil Nadu, India.
Mohamed Sadiq A Adhi parasakthi college of arts and science, Kalavai, Vellore Dt
MOHAMED SADIQ A Department of Biochemistry, Adhiparasakthi College of Arts and Science, Vellore, Tamil Nadu, India.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i6.33486

Keywords:

Nanoparticles,, Eco-friendly synthesis,, Electron microscopy,, Anticancer activity,, Huh7 cell lines,, Cellular staining

Abstract

Objective: The objective of the study was to access the anticancer activity of the biosynthesized ZnO nanoparticles against Huh7 liver cancer cell lines.

Methods: The study was carried in vitro using Huh7 cell lines. The ZnO nanoparticles (ZnO NPs) were synthesized using Luffa acutangula peel extract and subjected to characterization by X-ray powder diffraction and transmission electron microscopy. The Huh7 cell lines were treated with ZnO NPs and done 3-(4, 5-dimethylthiazol- 2-yl)-2, 5-diphenyltetrazolium bromide assay. For live and dead assay, the cell lines treated with ZnO NPs were subjected to acridine orange/ethidium (AO/ET) bromide assay.

Results: The ZnO NPs synthesized show spherical structure with 10–20 nm size. The 50% of Huh7 proliferation were inhibited at the concentration (IC50) of 40 μg/ml. The AO/ET assay shows compact nucleus and fine cytoplasmic morphology in control cells and apoptotic stage in treated cells

Conclusion: This study suggests that ZnO NPs can be prepared in environment-friendly method using aqueous extract of L. acutangula and can be used in cancer treatment effectively.

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