HYPOGLYCEMIC EFFECT OF HIGH-RESISTANT STARCH ANALOG RICE THROUGH GLP-1 AND INSULIN OR HIGH-RESISTANT STARCH ANALOG RICE ATTENUATES BLOOD GLUCOSE LEVEL THROUGH ENHANCEMENT OF GLP-1 AND INSULIN

HAIRRUDIN; SOETJIPTO; RETNO HANDAJANI

doi:10.22159/ajpcr.2019.v12i18.33944

Authors

HAIRRUDIN Department of Biochemistry, Faculty of Medicine, University of Jember, Indonesia.
SOETJIPTO Department of Medical Biochemistry, Faculty of Medicine, Universitas Airlangga, Indonesia.
RETNO HANDAJANI Department of Medical Biochemistry, Faculty of Medicine, Universitas Airlangga, Indonesia.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i18.33944

Keywords:

Analog rice,, Resistant starch,, Glucagon-like peptide-1,, Insulin,, Glucose transporter-2,, Nil

Abstract

Objective: This study was to investigate the effect of analog rice (AR) on glucagon-like peptide-1 (GLP-1) and insulin serum levels, glucose transporter-2 (GLUT-2) expression, and fasting blood glucose (FBG) level in diabetic rats.

Methods: Fifty male Wistar rats divided into the control group (n=10) and the experimental group. High-fat diet and streptozotocin were administered in experimental groups, which then divided into four equal groups (n=10, each) (negative control group, rice group, AR1 and AR2 group, given standard pellet, rice pellet, AR1 and AR2 pellet, respectively, for 6 weeks). GLP-1 and insulin serum levels were measured by enzyme-linked immunosorbent assay. The expression of GLUT-2 and the number of pancreatic β-cells observed using an immunohistochemistry method.

Results: FBG levels in the AR1 and AR2 groups decreased, while the rice group remained. GLP-1 serum levels of the negative control and rice groups were not significantly different from the control group, while the AR1 and AR2 groups higher than the control group (p≤0.05). All the treatment groups had insulin serum levels significantly lower than control group (p≤0.05), except the AR1 group. The expression of GLUT-2 and the number of pancreatic β-cells in the treatment groups were less than the control group, but between treatment groups were not significantly different.

Conclusion: AR significantly effective in reducing FBG level in diabetic rats through stimulation of increased GLP-1 and insulin serum levels serum levels but AR did not affect on the expression of GLUT-2.

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