NUTRITIONAL AND THERAPEUTIC EVALUATION OF SPIRULINA PLATENSIS

SACHIN KULKARNI; DEEPALI CHAVAN

doi:10.22159/ajpcr.2020.v13i7.34123

Authors

SACHIN KULKARNI Department of Biotechnology, Rajarshi Shahu College, Latur, Maharashtra, India.
DEEPALI CHAVAN Department of Biotechnology, Rajarshi Shahu College, Latur, Maharashtra, India.

DOI:

https://doi.org/10.22159/ajpcr.2020.v13i7.34123

Keywords:

Spirulina, Harvesting, Sulfate polyacrylamide gel, Protein estimation DNA isolation

Abstract

Objective: The present investigation was to isolate and produce Spirulina platensis on high scale for food pharmaceutical and aquaculture due to the presence of high protein content.

Methods: Cultivation of Spirulina preparation of culture medium, inoculums build up, growth monitoring, harvesting, drying, procedure for protein estimation, sulfate polyacrylamide gel electrophoresis (SDS-PAGE), and DNA isolation of Spirulina platensis.

Results: The study shows that protein content of S. platensis was found to be 62% protein profile was studied through SDS-PAGE, the bands were in the range of 35 kDa–44 kDa. S. platensis contain high molecular weight DNA. The bands was seen and visualize under the transilluminator it reveals that the alga S. platensis contain high molecular weight DNA.

Conclusion: The production of Spirulina by simple pH determination method suggests economic production of alga by a simple process. The DNA isolation showing that these algae contain high molecular weight DNA. However, utilization of this biomass for varied end uses, such as food, feed, aquaculture, and pharmaceuticals.

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Author Biography

DEEPALI CHAVAN, Department of Biotechnology, Rajarshi Shahu College, Latur, Maharashtra, India.

Pharmaceutical Biotechnology working as lecturer one of good college in shivaji university

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