PHYTOCHEMICAL SCREENING AND ANTIOXIDANT POTENTIAL OF ANACARDIUM OCCIDENTALE, ACHYRANTHES ASPERA, AND AEGLE MARMELOS

BRIJYOG; LALITESHWAR PRATAP SINGH; SUSHIL KUMAR; SHWETA VERMA

doi:10.22159/ajpcr.2019.v12i18.34147

Authors

BRIJYOG Department of Pharmacy, Institute of Pharmacy, Harish Chandra P.G. College Varanasi-221002, India, Institute of Pharmacy, Harish Chandra Postgraduate College, Varanasi, Uttar Pradesh, India,
LALITESHWAR PRATAP SINGH Department of Pharmacy, Institute of Pharmacy, Harish Chandra P.G. College Varanasi-221002, India, Institute of Pharmacy, Harish Chandra Postgraduate College, Varanasi, Uttar Pradesh, India,
SUSHIL KUMAR Department of Faculty of Pharmacy, IFTM University, Moradabad, U.P., India- 244102, Faculty of Pharmacy, IFTM University, Moradabad, Uttar Pradesh, India.
SHWETA VERMA Department of Faculty of Pharmacy, IFTM University, Moradabad, U.P., India- 244102, Faculty of Pharmacy, IFTM University, Moradabad, Uttar Pradesh, India.

DOI:

https://doi.org/10.22159/ajpcr.2019.v12i18.34147

Keywords:

Antioxidant activity, Flavonoids, Phenols, Anacardium occidentale, Achyranthes aspera, Aegle marmelos

Abstract

Objective: Anacardium occidentale, Achyranthes aspera, and Aegle marmelos are the common plants found in North and South India. The present study identifies the active phytochemicals and antioxidant properties in various extracts derived from dried bark, whole aerial parts, and leaves of the above plants.

Materials and Methods: Phytochemical screening included extracts of pet ether, hydroalcoholic, and aqueous. The antioxidant activity was determined by measuring total phenolic contents (TPC), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity (RSA), and total flavonoid content (TFC).

Results: The phytochemical screening of A. occidentale, A. aspera, and A. marmelos of dried bark, whole aerial parts, and leaves revealed the presence of flavonoids, phenols, tannins, and proteins. It also contains alkaloids and glycosides. Hydroalcoholic extract of A. occidentale, A. aspera, and A. marmelos showed highest TPC 0.125 mg/g, 0.256 mg/g, and 0.254 mg/g, respectively. TFCs 0.094 mg/g, 0.145 mg/g, and 0.121 mg/g and highest DPPH RSA with the half maximal inhibitory concentration of 125 μg/mL, 105.58 μg/mL, and 98.89 μg/mL compare to the standard ascorbic acid of 21.65 μg/mL.

Conclusion: This study showed that the hydroalcoholic extract of A. occidentale, A. aspera, and A. marmelos is potential source of natural antioxidants.

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References

Singh PS, Bal M, Mukhtar MH, Shah G. Standardization and pharmacological investigation on leaves of Ficus bengalensis. Int J Res Pharm Chem 2011;1:891-903.

Robbers JE, Tyler VE. Tyler’s Herbs of Choice: The Therapeutic use of Phytomedicinals. 3rd ed. New York: Haworth Press Inc.; 1999. p. 287-95.

Mukherjee PK. Quality Control of Herbal Drugs, an Approach to Evaluation of Botanicals. 1st ed. New Delhi: Business Horizon; 2002. p. 112-25.

Kujur RS, Singh V, Ram M, Yadava HN, Singh KK, Kumari S, et al. Antidiabetic activity and phytochemical screening of crude extract of Stevia rebaudiana in alloxan-induced diabetic rats. Pharmacogn Res 2010;2:258-63.

Saeed N, Khan MR, Shabbir M. Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts Torilis leptophylla L. BMC Complement Altern Med 2012;12:221.

Mythili K, Reddy CU, Chamundeeswari D, Manna PK. Determination of total phenol, alkaloid, flavonoid and tannin in different extracts of Calanthe triplicata. J Pharmacogn Phytochem 2014;2:40-4.

Biju J, Sulaiman CT, Satheesh G, Reddy VR. Total phenolics and flavonoids in selected medicinal plants from Kerala. Int J Pharm Pharm Sci 2014;6:406-8.

Kostic DA, Dimitrijevic DS, Mitic SS, Mitic MN, Stojanovic GS, Zivanovic AV. Phenolic content and antioxidant activities of fruit extracts of Morus nigra L (Moraceae) from Southeast Serbia. Trop J Pharm Res 2013;12:105-10.

Harborne JB. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis. Vol. 2. New York: Chapman and Hall; 1984. p. 288-95.

Braca A, Sortino C, Politi M, Morelli I, Mendez J. Antioxidant activity of flavonoids from Licania licaniae flora. J Ethnopharmacol 2002;79:379-81.

Helen LR, Jyothilakshmi M, Latha M. Antioxidant potential and antiproliferative effect on HCT-15cells. Int J Pharm Sci 2015;17:170-5.

Ahmad M, Shah AS, Khan RA, Khan FU, Khan NA, Shah MS, et al. Antioxidant and antibacterial activity of crude methanolic extract of Euphorbia prostrata collected from district Bannu (Pakistan). Afr J Pharm Pharmacol 2011;5:1175-8.

Ashalatha KS, Reddy AR, Raveesha HR. Evaluation of phytochemical compounds and antimicrobial activities of Hemidesmus indicus. Asian J Pharm Clin Res 2019;12:102-6.

Akila B, Manikandaselvi S. In vitro antioxidant and anticancer efficacies of ethanolic fruit extract of Ziziphus jujube mill. Asian J Pharm Clin Res2019;12:160-5.