INVESTIGATION OF THE EFFECTS OF KIGELIA AFRICANA (LAM.) BENTH. EXTRACTS ON TM3 LEYDIG CELLS
DOI:
https://doi.org/10.22159/ajpcr.2019.v12i10.34163Keywords:
Kigelia africana (Lam) Benth, Antioxidants, Cytotoxicity, TM3 Leydig cells testosteroneAbstract
Objective: The study reports the effects of acetone, methanol, and aqueous Kigelia africana extracts on the TM3 Leydig cells.
Methods: The total phenolic contents were determined using Folin–Ciocalteu’s method. Antioxidant activity was determined using 1,1-diphenyl-2- picrylhydrazyl assay and flavonoid content assessed using quercetin as standard. Cell viability and cytotoxicity were assessed.
Results: Total phenolic and flavonoid contents of the extracts were analyzed. Acetone extracts showed highest total phenolic content (105±3.65 mg gallic acid equivalent [GAE]/g) followed by methanol extracts (10.64±0.32 mg GAE/g) and, last, the water extract with the total phenolic content of 4.27±0.65 mg GAE/g. The same trend was observed with total flavonoid content where results recorded for acetone, methanol, and water extracts were 3.63 ±0.20 mg/g quercetin equivalent (QE), 0.16±0.01 mg QE/g, and 0.01±0.00 mg QE/g, respectively. The three extracts showed lower IC50 values compared to ascorbic acid (0.143±0.05 mg/ml, 0.023±0.004 mg/l, and 0.043±0.03 mg/ml for acetone, methanol, and water extracts, respectively). At 0.5 mg/ml of acetone extract, there was an increase in the testosterone production compared to the control.
Conclusion: The findings of this study showed that K. africana could be recommended for use in the treatment of male infertility.
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Patel SK, Singh S, Singh HK, Singh SK. Effect of standardized extract of Bacopa monnieri (CDRI-08) on testicular functions in adult male mice. J Ethnopharmacol 2017;197:101-9.
WHO EB134/24. Traditional Medicine. Executive Board, 134th Session; Provisional Agenda Item 9.1. 2013.
Wojdyło A, Oszmiański J, Czemerys R. Antioxidant activity and phenolic compounds in 32 selected herbs. Food Chem 2007;105:940-9.
Ansari N, Chande D. Antioxidant studies on methanol and aqueous extracts of Gymnosporia montana plant. Int J Pharm Pham Sci 2019;11:65-70.
Shalaweh SM, Erasmus N, Weitz F, Henke RR. Effect of Cissampelos capensis rhizome extract on human spermatozoa in vitro. Int J Androl 2014;20:1-10.
Ebrahim M, Pool EJ. The effect of Tulbaghia violacea extracts on testosterone secretion by testicular cell cultures. J Ethnopharmacol 2010;132:359-61.
Van Wyk BE. Medicinal Plants of South Africa. 2nd ed. Pretoria, South Africa: Briza; 2009. p. 184-5.
Pratima H. Antioxidant and antibacterial activity of alkaloid extract of Cucumis trigonus Roxb. Int J Pharm Pharm Sci 2019;11:44-8.
Lim TK. Fruits. In: Edible Medicinal and Non-Medicinal Plants. Vol. 2. Netherlands: Springer; 2012. p. 401.
Saini S, Kaur H, Verma B, Ripudaman S, Eloff NJ. Kigelia africana (Lam.) Benth an overview. Nat Prod Rad 2009;8:190-7.
Würger G, McGaw LJ, Eloff JN. Tannin content of leaf extracts of 53 trees used traditionally to treat diarrhoea is an important criterion in selecting species for further work. S Afr J Bot 2014;90:114-7.
Watt JM, Breyer-Brandwijk MG. The Medicinal and Poisonous Plants of Southern and Eastern Africa. 2nd ed. Edinburgh: E. and S. Livingstone Ltd.; 1962.
Makkar HP. Quantification of tannin in tree foliage. A Laboratory Manual For The Faq/Iaea Co-Ordinated Research Project on use for Nuclear and Related Techniques to Develop Simple Tannin Assay for Predicting and Improving the Safety and Efficiency of Feeding Ruminants on the Tanniferous Tree Foliage. Vienna, Austria: Joint FAQ/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna; 1999.
Adebayo SA, Dzoyem JP, Shai LJ, Eloff JN. The anti-inflammatory and antioxidant activity of 25 plant species used traditionally to treat pain in Southern African. BMC Complement Altern Med 2015;15:159.
Parthasarathy S, Bin Azizi J, Ramanathan S, Ismail S, Sasidharan S, Said MI, et al. Evaluation of antioxidant and antibacterial activities of aqueous, methanolic and alkaloid extracts from Mitragyna speciosa (Rubiaceae family) leaves. Molecules 2009;14:3964-74.
Yadav RN, Agarwal M. Phytochemical analysis of some medicinal plants. J Phytol 2011;3:10-14.
Miliauskas G, Venskutonis PR, Van Beek TA. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chem 2004;85:231-7.
Mensor LL, Menezes FS, Leitão GG, Reis AS, dos Santos TC, Coube CS, et al. Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytother Res 2001;15:127-30.
Hamzah RU, Jigam AA, Makun HA, Egwin EC. Phytochemical screening and in vitro antioxidant activity of methanolic extract of selected Nigerian vegetables. Asian J Basis Appl Sci 2014;1:1-14.
Monsees TK, Franz M, Gebhardt S, Winterstein U, Schill WB, Hayatpour J, et al. Sertoli cells as a target for reproductive hazards. Andrologia 2000;32:239-46.
Mahapatra A, Mativandlela SP, Binneman B, Fourie PB, Hamilton CJ, Meyer JJ, et al. Activity of 7-methyljuglone derivatives against mycobacterium tuberculosis and as subversive substrates for mycothiol disulfide reductase. Bioorg Med Chem 2007;15:7638-46.
Mosmann T. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.
Fadipe VO, Mongalo NI, Opoku AR. In vitro evaluation of the comprehensive antimicrobial and antioxidant properties of Curtisia dentata (Burm.f) C.A. Sm: Toxicological effect on the human embryonic kidney (HEK293) and human hepatocellular carcinoma (HepG2) cell lines. EXCLI J 2015;14:971-83.
Masoko P, Mmushi TJ, Mogashoa MM, Mokgotho MP, Mampuru LJ, Howard RL. In vitro evaluation of the antifungal activity of Sclerocarya birrea extracts against pathogenic yeasts. Afr J Biotechnol 2008;7:3521-6.
Złoteck U, Mikulska S, Nagajek M, Świeca M. The effect of different solvents and number of extraction steps on the polyphenol content and antioxidant capacity of basil leaves (Ocimum basilicum L.) extracts. Saudi J Biol Sci 2016;23:628-33.
Nemudzivhadi V, Masoko P. Antioxidant and antibacterial properties of Ziziphus mucronata and Ricinus communis leaves extracts. Afr J Tradit Comp Alt Med 2015;12:81-9.
Masoko P, Picard J, Eloff JN. Antifungal activities of six South African Terminalia species (Combretaceae). J Ethnopharmacol 2005;99:301-8.
Klimczak I, Malecka M, Gliszcyńska-Świglo A. Effect of storage on the content of polyphenols, vitamins and the antioxidant activity of orange juices. J Food Compos Anal 2007;20:313-22.
Tahara S. A journey of twenty-five years through the ecological biochemistry of flavonoids. Biosci Biotechnol Biochem 2007;71:1387-404.
Giorgi A, Mingozzi M, Madeo M, Speranza G, Cocucci M. Effect of nitrogen starvation on the phenolic metabolism and antioxidant properties of yarrow (Achillea collina Becker ex Rchb.) Food Chem 2009;114:204-11.
Eloff JN, McGaw LJ. Plant extracts used to manage bacterial, fungal and parasitic infections in Southern Africa. In: Ahmad I, Owais M, editors. Modern Phytomedicine: Turning Medicinal Plants into Drugs. Weinheim: Wiley-VCH Verlag GmbH and Co. KGaA; 2006. p. 113.
Ak T, Gülçin I. Antioxidant and radical scavenging properties of curcumin. Chem Biol Interact 2008;174:27-37.
Floegel A, Kim DO, Chung SJ, Koo SI, Chun OK. Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods. J Food Compos Anal 2011;24:1043-8.
Krishnaiah D, Sarbatly R, Nithyanandam R. A review of the antioxidant potential of medicinal plant species. Food Bioprod Process 2011;89:217-33.
Do QD, Angkawijaya AE, Tran-Nguyen PL, Huynh LH, Soetaredjo FE, Ismadji S, et al. Effect of extraction solvent on total phenol content, total flavonoid content, and antioxidant activity of Limnophila aromatica. J Food Drug Anal 2014;22:296-302.
EI-Chaghaby GA, Ahmad AF, Ramis ES. Evaluation of the antioxidant and antibacterial properties of various solvents extracts of Annona squamosal L. leaves. Arabian J Chem 2014;7:227-33.
Abdulkadir MN, Adedokun A, John E. Phytochemical composition and antimicrobial evaluation of Kigelia africana LAM. Asian J Plant Sci Res 2015;5:14-7.
Galano A, Mazzone G, Alvarez-Diduk R, Marino T, Alvarez-Idaboy JR, Russo N, et al. Food antioxidants: Chemical insights at the molecular level. Annu Rev Food Sci Technol 2016;7:335-52.
Nimse SB, Pal D. Free radicals, natural antioxidants, and their reaction mechanisms. RSC Adv 2015;5:27986-8006.
Fotakis G, Timbrell JA. In vitro cytotoxicity assays: Comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicol Lett 2006;160:171-7.
Opuwari CS, Monsees TK. Reduced testosterone production in TM3 leydig cells treated with Aspalathus linearis (Rooibos) or Camellia sinensis (tea). Andrologia 2015;47:52-8.
Petersen PM, Seierøe K, Pakkenberg B. The total number of leydig and sertoli cells in the testes of men across various age groups a stereological study. J Anat 2015;226:175-9.
Walker WH. Testosterone signaling and the regulation of spermatogenesis. Spermatogenesis 2011;1:116-20.
Azu OO, Duru FI, Osinubi AA, Noronha CC. Histomorphometric effects of Kigelia africana (Bignoniaceae) fruit extract on the testis following short-term treatment with cisplatinin male sprague-dawley rats. Mid East Fert Soc J 2010;15:200-8.
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